Revised Matrix / Table of Specifications (TOS) for the Licensure Examination (Finalized with PASMETH & PAMET on June

6, 2009) SUBJECT & TOTAL WEIGHT Clinical Chemistry (20%) 1 2 3 4 5 Specimen collection Instrumentation (Principles, Methods, Calibration, Others) Reagent preparation and laboratory mathematics Quality assurance Metabolic Blood Tests (Principles, Procedures, Diseases/Disorders, Reference values) 5.1. Water balance and electrolytes 5.2. NPN and other metabolic intermediaries and inorganic ions 5.3. Carbohydrates 5.4. Lipids and dysproteinemia 5.5. Specific proteins 5.6. Liver function tests 5.7. Clinical enzymology Endocrinology and Toxicology (Principles, Procedures, Diseases/Disorders, Reference values) 6.1. Endocrinology 6.1.1. Thyroid Hormones 5% 5% 5% 10% 50% 8% 8% 6% 8% 6% 6% 8% 16% 4% TOPIC & SUB-TOPIC WEIGHT (%)

7 8

6.1.2. Sex Hormones 6.1.3. Other Hormones (Pituitary, Adrenal) 6.2. Toxicology & Therapeutic Drug Monitoring (TDM) 6.2.1. Substance of Abuse 6.2.2. Other poisons/toxic agents (Alcohol, Carbon monoxide, Mercury, Lead, Arsenic) 6.2.3. TDM - Anticonvulsants and other drugs Blood gas analysis and other tests (Principles, Procedures, Diseases/ Disorders, Reference values) Laboratory safety Total

3% 3% 2% 2% 2% 4% 5% 100% 5% 30% 15% 10% 5% 40% 6% 12% 12% 10% 20% 2% 8% 10% 5%

Hematology (20%) 1 2 Blood collection, anticoagulants and others (including Safety) Hematology tests and procedures 2.1. Routine 2.2. Automation 2.3. Special Hematopoiesis, Diseases/Disorders and Reference values 3.1. Hematopoiesis (in general) 3.2. Erythropoiesis and RBCs 3.3. Leukopoiesis and WBCs 3.4. Thrombopoiesis and Platelets Coagulation (Principles, Procedures, Diseases/Disorders and Reference values) 4.1. Hemostasis - Theories/Concepts, Mechanisms 4.2. Coagulation procedures/tests 4.3. Coagulation factors, diseases/disorders & reference values Quality assurance

Total Blood Banking, Immunology and Serology (20%) 1 2 3 4 5 6 7 8 9 10 11 12 13 Blood Banking (50%) ABO and Rh Blood Group Systems Other Major Blood Group Systems: Kell, Duffy, Kidd, Lewis, MNSs, Lutheran, P, I Minor Blood Group Systems: Diego, Cartwright, Chido, XG, Scianna, Gerbich, Milton, Knops, Bg, Indian, etc Basic Genetics Blood donor selection and processing Blood preservation and banking Component preparation Transfusion therapy Transfusion reactions Transfusion-transmitted diseases BB techniques and procedures : typing, compatibility testing, antibody detection and identification Hemolytic Disease of the Newborn (HDN) and Auto-immune Hemolytic Anemia Quality management (structure, set-up/ equipment, Laboratory Information System/LIS) Sub-Total

100%

5% 3% 1% 2% 5% 5% 5% 2% 3% 3% 8% 4% 4% 50%

Immunology/Serology (50%) 14 15 Historical background Natural (innate) immunity, including role of macrophages, monocytes and granulocytes 2% 5%

16 17 18 19 20

21 22 23

Acquired immunity - humoral responses, immunogens, immunoglobulins, B cells Acquired immunity - cellular responses, T cells, cytokines and chemokines Complement System MHC, HLA and Transplantation Immunologic tests for detection of antigens & antibodies - principles, procedures, interpretation of results 20.1. Bacterial infections and STD 20.2. Viral infections, including Hepatitis and HIV 20.3. Fungal infections 20.4. Parasitic infections, including malaria 20.5. Autoimmune disorders Tumor immunology (Tumor markers, Oncoproteins) Hypersensitivity Instrumentation and quality management Sub-Total Total

8% 5% 2% 3% 16% 5% 5% 1% 2% 3% 3% 1% 5% 50% 100%

Clinical Microscopy (10%) 1 Urine 1.1. Anatomy and physiology of the kidney Formation of urine 1.2. Macroscopic examination 1.3. Chemical analyses 1.4. Microscopic examination 1.5. Pregnancy testing 1.6. Renal calculi 53% 5% 10% 18% 15% 2% 3%

2 3

4 5 6

Feces Other Body Fluids 3.1. CSF 3.2. Seminal Fluid 3.3. Amniotic Fluid 3.4. Gastric Fluid and Duodenal Content 3.5. Sputum and Bronchial Washings 3.6. Synovial Fluid 3.7 Peritoneal, Pleural, and Pericardial Fluids Collection, preservation and handling of specimens Microscope, automation, other instruments Quality assurance and laboratory safety Total

3% 21% 5% 5% 3% 2% 2% 2% 2% 10% 5% 8% 100%

Microbiology/ Parasitology (20%) 1

Microbiology (70%) Bacteriology 1.1. Collection, transport , processing and staining of specimens 1.2. Culture media 1.3. Bacteria (Aerobes) 1.3.1. Morphology and staining characteristics 1.3.2. Cultural characteristics 1.3.3. Work-up for identification: biochemical, differential and confirmatory tests 1.3.4. Serologic/molecular tests 1.3.5. Susceptibility tests 1.3.6. Bacteriologic examination of water, food, milk, and utensils 1.4. Bacteria (Anaerobes) 1.5. Mycobacteria 1.6. Other bacteria with unusual growth requirements (Spirochetes, Chlamydia, Mycoplasma, Rickettsia) Mycology 2.1. Collection, transport and examination of clinical specimens 2.2. Culture Virology 3.1. General characteristics, transmission and diseases 3.2. Collection, transport and examination of clinical specimens 49% 5% 5% 5% 5% 14% 3% 4% 2% 2% 2% 2% 4% 2% 2% 4% 2% 2%

2 3

4 5

Equipment and instrumentation 4.1. Manual 4.2. Automated Quality assurance and safety 5.1. Collection of specimen 5.2. Quality control 5.3. Safety - patient/staff 5.4. Safety - workplace/environment Sub-Total Parasitology (30%) Parasites - life cycle, morphological characteristics,epidemiology, prevention and control, manner of reporting, counting 6.1. Nematodes 6.2. Trematodes 6.3. Cestodes 6.4. Protozoa 6.5. Ectoparasites Parasitologic Techniques 7.1. Routine 7.2. Concentration 7.3. Others Quality assurance 8.1. Collection and preservation of specimen 8.2. Quality control Sub-Total Total

5% 3% 2% 8% 2% 2% 2% 2% 70% 21% 5% 5% 5% 5% 1% 5% 2% 2% 1% 4% 2% 2% 30% 100%

Histopath, MT Laws, Related Laws & Code of Ethics (10%)

Histopathology (65%) 1 Histology and Pathology 1.1. Terminologies 1.2. Etiology of disease 1.3. Signs, symptoms and course of disease 1.4. Cellular and tissue changes Histopathologic techniques and procedures 2.1. Preservation and handling of specimen 2.2. Tissue processing and procedures 2.2.1. Routine- Manual 2.2.2. Routine- Automation 2.2.3. Special - Frozen section, Microwave 2.3. Staining 2.3.1. Routine 2.3.2. Special (Immunohistochemistry) Cytological techniques and procedures 3.1. Preservation and handling of specimen 3.2. Processing 3.2.1. Manual 3.2.2. Automation 3.3. Staining Autopsy 4.1. Terminologies 4.2. Handling, processing and documentation Quality assurance Sub-Total MT Laws, Related Laws and Code of Ethics (35%) 6 MT Laws 10% 10% 4% 2% 2% 2% 35% 10% 7% 5% 3% 5% 5% 8% 2% 2% 2% 2% 2% 1% 1% 10% 65%

4 5

7 8 9

Laboratory Management Related Laws Code of Ethics including Bioethics Sub-Total Total

10% 10% 5% 35% 100%