Conclusion and Evaluation

The patterns and trends stated in data are that the beans or peas volume are continuously decreasing while the marbles, control variable, is almost stable or decreasing slightly. From this trend we can assume that the beans and peas are absorbing oxygen. According to AP Biology 5: Cell Respiration http://www.scribd.com/doc/7570252/APBiology-Lab-Five-Cell-Respiration, the data shows same pattern as mine. For beads (marbles for mine), the volume was constant at 1.38 mL, while the germinating peas were dropping from 1.35 mL to 0.57 mL. The second trial of this data shows same trend as the first trial. The cells needs to do cellular respiration to have energy called ATP. This is done by breaking down nutrient molecules, and gas exchange between oxygen and carbon dioxide. While doing aerobic respiration, which is a type of cellular respiration, the cell absorbs oxygen and emits carbon dioxide. In this experiment, the cotton with KOH absorbs the carbon dioxide. Than, the amount of oxygen absorbed by the peas and beans are valid, but not the amount of carbon dioxide emitted by the cells. The pipet is inside of the water which helps to sustain the continuous temperature and the gas pressure inside. The temperature is important because the chemical reaction and enzymes or body functions are heavily dependent on the temperature. Therefore, the liquid inside of pipet goes down toward the water when the beans and peas do cellular respiration. In my data, the number for liquid written in green beans and red peas pipets was moving down closer to the beans and peas. For trial 1, the liquid in 5 green peas pipet went down to 0.09 mm from 0.30 mm, while marbles went to from 0.40 mm to 0.34 mm. In trial 2, the liquid in 5

red beans went down to 0.11 mm from 0.36 mm, while marbles went 0.66 mm to 0.50 mm. The pattern is same for trial 3 and 4. The results supports my hypothesis that the germinated beans and peas require oxygen to do cellular respiration, and the cellular respiration is necessary for getting energy called ATP to sustain their lives.

Evaluation

Improvements

- The error bars for the pipet is 1 mm. The - Next time, I should be more conscious on standard deviations are all less than 0.01 that the temperature - CV. The temperature of the probability of variation or mistake is water and the air should be near 37 degree very little. which is optimal temperature for most of - As I explained above, the data is sufficient enzymes. In this way, I can get the more to address aim/RQ that the beans or peas accurate and valid data for the experiments. absorb oxygen and do gas exchange for - The peas and beans - IV, and marbles - DVcellular respiration. should not be counted by the number, but - The IV is just counting unit of peas and measured by the volume and surface area. beans. It is easier to do it in small size - Since the RQ/aim is to observe the oxygen experiment and effective because most of taken by the peas and beans for cellular beans have similar volume and surface area; respiration by comparing to the marbles however, it is not effective for larger size which is not composed of living cells. amount. It is rather better to measure the volume of the beans and peas. It is because the amount of cell correlates to the volume and surface area, which determines the rate of gas exchange by cellular respiration. - The random biological variation is not valid for this experiment. - The instrumental errors can be 1 mm for measuring pipet. Also, the air can not be capped tightly that the air can freely go out and in. - The systemic errors are not valid, since the method was given.