SPE

G P s trata-X

T he s trata X Method
s trata-X is a revolutionary, patent-pending polymeric s olid phas e extraction (S P E ) s orbent. T his s imple, general extraction method relies upon the unique polarity of the s trata-X s orbent to retain a wide range of acidic, neutral and bas ic compounds from aqueous matrices by utilizing a combination of hydrophobic, hydrophilic and retention mechanis ms . It is des igned to s erve as a s tarting point and may be optimized for s pecific analytes .
Table 1. T he phys ic al and chemic al charac teris tic s of s trata-X P has e: P article s ize: P ore s ize: S urface area: pH s tability: s urface modified s tyrene-divinylbenzene polymer 33 m 85 800m2/g 1-14

S pec imen preparation: urine: plas ma/s erum: other aqueous s amples :

dilute urine with an equal volume of 100mM phos phate buffer (pH = 6.0). B e aware that many compounds are excreted as glucuronide metabolites from urine and may need hydrolys is . dilute 1:1 with deionized (DI) water or 100mM phos phate buffer (pH = 6.0). B e aware that the target compound maybe protein bound and may require an additional protein dis ruption s tep prior to extraction. adjus t pH to 5.5-7.5.

T he s trata-X method for general s c reening:

T he volumes s hown are for 30mg s orbent mas s . methanol / acetonitrile (1:1)

C ondition
with 1mL methanol

E quilibrate
with 1mL D.I. water

L oad S ample

Was h Impurities
with 1mL 5% methanol in D.I. water and dry 30 s ec at full vacuum

E lute A nalyte!
with 1mL methanol:acetonitrile (1:1)

Optimize the s trata-X method P olar c ompounds : Was h: 10-20% acetonitrile (or methanol) in water E lution: methanol/acetonitrile/water/acid (60:30:10:0.1) A c idic c ompounds : Was h: 2% acetic acid in methanol/water (5:95) E lution: 2% ammonium hydroxide in methanol/water (50:50) B as ic c ompounds : Was h: 2% ammonium hydroxide in methanol/water (5:95) E lution: 2% acetic acid in methanol/water (50:50)

C omments
O ptimizes the method for more aggres s ive clean-up of the interferences during the was h s tep and to elute the s tubborn, s trongly retained polar analytes . E ns ures the pH of the s olution in the was h s tep is kept low s o that the acidic compounds remain neutralized, increas ing their retention. T his allows for aggres s ive clea n-up of interferences . B as e is added to the elution s olvent facilitating the extraction of the analytes . E ns ures the pH of the s olution in the was h s tep is kept high s o that the bas ic compounds remain neutralized, increas ing their retention. T his allows for aggres s ive clea n-up of interferences. T he pH of the elution s olvent is lowered by adding acid, facilitating the extraction of the analytes .