LS23L Spring 2012 Syllabus

Revised March 27 2012

LS23L: INTRODUCTION TO LABORATORY & SCIENTIFIC METHODOLOGY (2 UNITS) SPRING 2012 SYLLABUS WHO SHOULD TAKE THIS COURSE? This course is the new Life Sciences Laboratory intended for students who took the prerequisite course LS2 starting in Fall 2011, and who are concurrently enrolled in LS3. Chem 14C is an enforced prerequisite for LS23L and LS3. INTRODUCTION This course is an introductory life sciences laboratory designed for undergraduate students. LS23L offers the opportunity to conduct wet-lab and cutting-edge bioinformatics laboratory experiments. This course has a one-hour online reading assignment/quiz and three hours of laboratory research each week (for a total of 4 hours per week). Undergraduate students will work in groups of three conducting experiments in the areas of physiology, metabolism, cell biology, molecular biology, genotyping and bioinformatics. INSTRUCTOR LAB MANAGER LAB COORDINATOR Gaston M.U. Pfluegl 2875 Slichter Hall Rhonda Murotake 2344 Young Hall Rachel Sauvageot 2344 Young Hall drpfluegl@gmail.com (310) 794-4113 RhondaM@lifesci.ucla.edu RachelS@lifesci.ucla.edu

LECTURE & LAB MEETINGS LABORATORY RESEARCH

Online Materials One 3-hour section on Tuesday, Wednesday, or Thursday in YHS 2370, 2336, 2379 Consult the LS23L CCLE page for your exact laboratory classroom. http://www.lsic.ucla.edu/classes/spring12/ http://lslab.lscore.ucla.edu/

COURSE WEBSITE LAB WEBSITE

ONLINE QUIZZES AND RESOURCES http://www.ccle.ucla.edu/ WEEK-BY-WEEK ASSIGNMENTS http://lslab.lscore.ucla.edu/LAB/INFO/LS23LW.htm

TEXT BOOK, TITLE, AUTHOR, AND YEAR: Life Sciences LS23L, Introduction to Laboratory & Scientific Methodology, Hayden McNeil, first edition, Pfluegl, 2011, ISBN 978-0-7380-5113-0 COURSE REQUIREMENTS Prerequisite: LS2, Chem14C. LS23L students must participate in one three-hour laboratory session per week. In addition, students are required to review online materials before laboratory and complete a quiz based on the laboratory manual. There will be an online multiple-choice final during finals week.
LS23L Spring 2012 Preview Syllabus

LS23L Spring 2012 Syllabus

Revised March 27 2012

Co-requisite: LS3. Concurrent enrollment in LS3, Introduction to Molecular Biology. GRADING Three individual written laboratory reports due in lab the following week (Weeks 1, 4 and 7). Seven in-class worksheets due at end of laboratory (Weeks 2, 3, 5, 6 & 10). Also 8 & 9? Ten pre-lab reading quizzes to be completed online (all Labs). Ten in-class quizzes at beginning of laboratory (all Labs). Ten post-lab quizzes to be completed before the end of the quarter (all Labs). Multiple-choice final exam. PRE-LAB READING QUIZZES Pre-lab reading quizzes will be based on the lab manual content and any assigned video content. You should expect to spend about 50 minutes total studying the assigned content and taking the multiple choice quiz on CCLE. Each quiz will open the Friday before a lab week and must be completed by noon on the Monday of lab week. Please see the weekly information grid for more details. All due dates are subject to change. No make-up quizzes will be granted. You alone are responsible for making sure all assignments are completed on time. Each pre-lab quiz is worth 3 points. IN-LAB QUIZZES In-lab quizzes are based on the lab manual content and any assigned video content. They are approximately 5 minutes long and are taken by all students at the beginning of each laboratory section. No extra time will be given for tardiness. Each in-lab quiz is worth 8 points. LAB ASSIGNMENTS Each lab will have an associated assignment worth 30 points: either a lab report or a worksheet. Written lab reports will be due one week following the lab, at the beginning of your next lab section. You will be working in groups of three; however, the written assignments are individual assignments. This means you can share data but NOT copy your partners written lab reports. Worksheets will be due at the end of the lab section, before you leave the room. At the end of the quarter, your lowest lab assignment score will be dropped, with the exception of the assignment for the DNA Isolation & Amplification Lab, which cannot be dropped. POST-LAB QUIZZES Post-lab reading quizzes will be based on the laboratory itself, and should be taken on CCLE after completing the lab. Each post-lab quiz is worth 2 points and will be available after the last lab section of the week on Thursdays. The post-lab quizzes will remain open until finals week. FINAL EXAM The comprehensive final exam is a multiple choice online test taken during finals week and is worth 100 points.

LS23L Spring 2012 Preview Syllabus

LS23L Spring 2012 Syllabus

Revised March 27 2012

LAB SAFETY & PERSONAL PROTECTIVE EQUIPMENT (PPE) Safety in the lab is of paramount importance. There is absolutely no food or drink allowed in the laboratory and no food or drink waste may be disposed of in the laboratory trashAll labs on campus must comply with UCLA policies and procedures. Proper laboratory attire is required at all times. While in a designated lab room, your legs and feet must be completely covered by long pants/skirt and closed toe shoes. Students who do not comply will not be allowed to enter the lab room and will not be granted a make-up lab. Additional PPE, including a lab coat, must be worn whenever chemicals are present in the room (most labs during the quarter). It must have full length sleeves that come to the wrist. You are responsible for bringing your own lab coat each week. There are no loaner lab coats available. Lab coats can be purchased from the AXE chemistry fraternity (located in 1275 Young Hall), at Scrubs Unlimited (located at 10930 Weyburn Ave in Westwood Village) or at the Health Sciences Store on campus. Students arriving to lab without a lab coat will not be allowed to remain in the room. Goggles and gloves will be provided in the laboratory. If you have questions about lab attire and PPE requirements, please review UCLA Policy 905, available online. http://www.adminpolicies.ucla.edu/pdf/905.pdf ATTENDANCE AND MAKE-UP ASSIGNMENTS POLICY Weekly attendance in your enrolled lab section is MANDATORY. There are no make-up labs. If you have a legitimate, excused absence (with documentation), lab points can be prorated. If you miss a lab unexcused, you will NOT be allowed to complete the lab assignment for credit. If you are sick or otherwise unable to attend your scheduled lab section, you must email your TA as soon as possible. ACADEMIC INTEGRITY Students are expected to be aware of the University of California policy on academic integrity pursuant to the UCLA Student Conduct Code (www.deanofstudents.ucla.edu). Please review sections on (1) plagiarism, (2) cheating and (3) use of unauthorized study aids. Violations are promptly referred to the Dean of Students. The Academic Senate requires instructors to report to the Dean of Students any student suspected of academic dishonesty. Penalties for such violation of the UCLA Student Conduct Code can be quite severe and may affect your graduate or professional school plans.

LS23L Spring 2012 Preview Syllabus

LS23L Spring 2012 Syllabus

Revised March 27 2012

LAB SCHEDULE AND POINT ALLOCATION TOPIC WEEK 1 (APR 1-7) Introduction to Scientific Methodology WEEK 2 (APR 8-14) Pipetter Exercise & Analysis of Protein Size Using SDS-PAGE WEEK 3 (APR 15-21) DNA Isolation & Amplification WEEK 4 (APR 22-28) Pigments of Photosynthesis WEEK 5 (APR 29-MAY 5) b-Galactosidase Activity Assay WEEK 6 (MAY 6-12) Agarose Gel Electrophoresis & Molecular Clocks WEEK 7 (MAY 13-19) Metabolism in Goldfish WEEK 8 (MAY 20-26) Rat Dissection WEEK 9 (MAY 27-JUN 2) Histology WEEK 10 (JUN 3-9) Sequence Analysis and Maternal Lineages Lowest lab assignment score will be dropped *Week 3 DNA Isolation & Amplification assignment score CANNOT be dropped) 100 Online multiple choice lab final 500 TOTAL PRE-LAB QUIZ 3 IN-LAB QUIZ 8 POST-LAB ASSIGNMENTS QUIZ 2 30

30

30

30

30

30*

30

30

30

30

-30

LS23L Spring 2012 Preview Syllabus

LS23L Spring 2012 Syllabus

Revised March 27 2012

LS23L Spring 2012 Preview Syllabus

Week

Lab

Before Lab - Read pages 1 - 14 in the lab manual - Watch the MIT video on the lab website http://lslab.lscore.ucla.edu/LAB/Video/ (approximately 3 minutes long, with sound) - Read the IRB, background and information pages provided on the lab website. http://lslab.lscore.ucla.edu/MIT/index.php - Take the pre-lab quiz on CCLE by Monday of lab week at noon - Read pages 55 - 61 in the lab manual

In Lab

Assignment

Introduction to Scientific Methodology

- Labs will meet on the fourth floor of Young Hall South, in the Student Learning Center - Write an individual lab report and proposal (SLC). To find your room for this week, for further research based on the guidelines please consult the room grid provided on given in the lab manual (pg 14). CCLE. - Both assignments will be due at the - An eight point multiple choice quiz will be beginning of your lab section the following administered at the beginning of your lab week. section.

Pipetting Exercise and Analysis of - Watch the safety video on the lab website Protein Size and (approximately 20 minutes long, with sound) Subunit - An eight point multiple choice quiz will be Composition - Take the pre-lab quiz on CCLE by Monday administered at the beginning of your lab of lab week at noon. section. - Labs will meet in the assigned lab rooms (located on the second floor of Young Hall South). Proper lab attire and lab coat are required. - An eight point multiple choice quiz will be administered at the beginning of your lab section. - Labs will meet in the assigned lab rooms (located on the second floor of Young Hall South). Proper lab attire and lab coat are required. - An eight point multiple choice quiz will be administered at the beginning of your lab section.

- Labs will meet in the assigned lab rooms (located on the second floor of Young Hall South). Proper lab attire and lab coat are required.

- You will complete a worksheet during your lab section and turn it in before you leave the room.

- Read pages 71 - 80 in the lab manual DNA Isolation and Amplification - Take the pre-lab quiz on CCLE by Monday of lab week at noon.

- You will complete a worksheet during your lab section and turn it in before you leave the room.

- Read pages 17 - 25 in the lab manual 4 The Pigments of Photosynthesis - Take the pre-lab quiz on CCLE by Monday of lab week at noon

- Write an individual lab report based on the guidelines given in the lab manual (pg 25). - Your lab report will be due at the beginning of your lab section the following week.

- Read pages 63 - 69 in the lab manual Biochemical Assay of b-Galactosidase - Take the pre-lab quiz on CCLE by Monday of lab week at noon

- Labs will meet in the assigned lab rooms (located on the second floor of Young Hall South). Proper lab attire and lab coat are required. - An eight point multiple choice quiz will be administered at the beginning of your lab section. - Labs will meet in the assigned lab rooms (located on the second floor of Young Hall South). Proper lab attire and lab coat are required. - An eight point multiple choice quiz will be administered at the beginning of your lab section. - Labs will meet in the assigned lab rooms (located on the second floor of Young Hall South). Proper lab attire and lab coat are required. - An eight point multiple choice quiz will be administered at the beginning of your lab section. - Labs will meet in the assigned lab rooms (located on the second floor of Young Hall South). Proper lab attire and lab coat are required. - An eight point multiple choice quiz will be administered at the beginning of your lab section. . - Labs will meet in the assigned lab rooms (located on the second floor of Young Hall South). Proper lab attire and lab coat are required. - An eight point multiple choice quiz will be administered at the beginning of your lab section.

- You will complete a worksheet during your lab section and turn it in before you leave the room.

Agarose Gel Electrophoresis and Molecular Clocks

- Read pages 81 - 88 in the lab manual - Take the pre-lab quiz on CCLE by Monday of lab week at noon

- You will complete two worksheets during your lab section and turn them in before you leave the room.

- Read pages 27 - 34 in the lab manual 7 Metabolism in Goldfish - Take the pre-lab quiz on CCLE by Monday of lab week at noon.

- Write an individual lab report based on the guidelines given in the lab manual (pg 34). - Your lab report will be due at the beginning of your lab section the following week.

- Read pages 35 - 45 in the lab manual 8 Rat Dissection - Take the pre-lab quiz on CCLE by Monday of lab week at noon.

- At the end of lab you will take a quiz that will ask you to identify rat anatomical structures and functions.

- Read pages 47 - 53 in the lab manual 9 Microscopy and Histology - Take the pre-lab quiz on CCLE by Monday of lab week at noon

- Working with your group, you will complete a worksheet and turn it in before you leave the room. All group members will receive the same score.

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11

- Labs will meet on the fourth floor of Young Hall South, in the Student Learning Center (SLC). To find your room for this week, - Read pages 89 - 101 in the lab manual Sequence Analysis please consult the room grid provided on - You will complete two worksheets during and Maternal CCLE. your lab section and turn them in before you - Take the pre-lab quiz on CCLE by Monday Lineages leave the room. of lab week at noon - An eight point multiple choice quiz will be administered at the beginning of your lab section. The comprehensive final exam will be taken online during finals week and will be worth 100 points. Additional information will be provided FINALS WEEK later in the quarter.