Experiment Flow Chart: Exploring Biology Lab, Julie Casteel in Bench #3

Unconditioned Stimulus Paired: A Warning Stimulus like a flash of light, then a Stronger Stimulus Applied in the form of adverse shocks or auditory noise applied. Unconditioned Response or Reaction to Paired Stimulus: the warning stimulus will not produce a response alone while the stronger stimulus will produce a stronger response
Measure Temporal Response Patterns in Auditory Evoked Potentials. Oxygen saturation as a measure of respiration rate also suffices .

Repeat on predictable, constant intervals: measure each number of trials (vertical component) over impulses per msec (horizontal component) to track progress with annotated parts to show when each stimulus was introduced

Classicial Conditioning and motor learning take place through "training"

When the warning stimulus or flash of light is introduced, fish will anticipate or predict an auditory stimulus is coming .

Fish have created an association with the warning light.

If they the warning stimulus alone, then because of the association, they will illict the same response as they would for the second, stronger stimulus in auditory evoked potentials.

Compare the learniing and conditioning of two groups. Specifically, measure the difference in the response to the warning throughout learning and compare the response an auditory stimulus generates. An increased response would suggest that there is an increased amount of activity: neurons are buzzing and processing is happening.

Variable Group: Coral bleaching in environment is better "coped", adapted, or tolera by coral because of some unique difference others don't have.

Control group: Coral bleaching in environment is stress that isn't being coped with. Impacts on bioddiversity, the ecosystem, species density, and mortality of coral seen.

IF INCREASED LEVELS OF CO2 CORRELATE WITH DIMINISHED COGNITIVE ABILITIES AND LEARNING FERRARI, ET. AL 2012 THEN FISH IN ENVIRONMENTS WHERE BACKBONE (CORAL REEF) OF THE HABITAT ARE ADAPTING THEN AN INCREASED TOLERANCE TO ACIDIFICATION IN OCEANS WILL BE MEASURED. WE KNOW THAT NEUROSIGNALS AND CHEMICALS (NEVITT 2010) IN THIS ISOLATED BIOME EFFECTY EACHOTHER AS WELL AS THE LARGER WORLD AT LARGE. AS WELL AS THAT SPECIES LIKE CERTAIN FISH SPECIES ARE DEPENDENT UPON CORAL HABITATS FOR LIFE.

I HYPOTHEISZE THAT THIS WOULD SUPPORT A MECHANISM FOR FISH TO LEARN/ADAPT/RESPOND/PROCESS THE STRESSORS IN ENVIRONMENTS BY THE EXAMPLE OF CORAL PAVING THE WAY AS WELL AS A CO-EVOLUTION PROCESSESED ADAPTATION. CORAL ARE "LEADING BY EXAMPLE" AND BECAUSE OF THE STRONG DEPENDENCE ON ONE ANOTHER CHANGES IN CORAL CAUSE CHANGES IN REEF FISH.

Null Hypothesis: The control and variable groups are independent of one another. There is no edge held in learning and cognitive functioning of one group over another. Alternate Hypothesis: The responses and learning mechanisms are dependent upon what is going on in their environment. FIsh in environments where coral species are adapting learn quicker, more, appropriately, and exhibit some "edge" over control .

Conclusions Do chi-squre tests, hypothesis statement testing to make conclusions and results.

Apply coral transplantation remediation recover efforts to both environments and repeat: How does learning change with recovery methods: is transplatation a good solution? What about the role of plants like phytoplankton or other things that could explain it?