Weight (g) Replications 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 n Mean Std Dev 2 * Std Dev Observed Min Observed Max Lower CI Upper CI 1% error

Min 1% error Max Performance OK?

P20 10 10.05 9.95 10.01 10.02 9.98 9.95 10.13 9.87 10 10.01 9.99 10 10 10.02 9.98 10.01 10.01 10.01 10.01 20 10 0.047903412 0.095806823 9.87 10.13 9.904193177 10.09580682 9.9 10.1 OK

P200 100 100.5 99.5 100.1 100.2 99.8 99.5 101.3 98.7 100 100.1 99.9 100 100 100.2 99.8 100.1 100.1 100.1 100.1 20 100 0.479034116 0.958068232 98.7 101.3 99.04193177 100.9580682 99 101 OK

P1000 500 502.5 497.5 500.5 501 499 497.5 506.5 493.5 500 500.5 499.5 500 500 501 499 500.5 500.5 500.5 500.5 20 500 2.39517058 4.790341159 493.5 506.5 495.2096588 504.7903412 495 505 OK

1 Introduction to Recombinant Genetics- Biology 350 Index Syllabus CourseInfo LogIn References Assignment Next
Sampling, Mean and Variance Answer How can you tell if two populations are different? Answer LAB 1 - STATISTICS OF PIPETTING Exercise 1 This exercise has three purposes, 1) it will help you refine your pipetting skills, 2) it will determine if your micropipettes are performing properly, 3) it will allow you to practice some basic statistics using spreadsheets. Download the Excel spreadsheet for Lab 1. and open the Lab1 Pipette Calibration sheet (tab 1).

Your instructor will explain how to use some of the functions shown below. Your instructor will give you three micropipettes. Set the P20 to 10L, the P200 to 100 L and the P1000 to 500 L. Each person will check two different pipettes. You will tare the balance and then pipette deionized water onto a balance and record the weight (in mgs) below. Tare the balance before each sample is added. Repeat this for each micropipette 20 times and record your data below. Weight (mg) = Volume (l) Replications 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 n Mean Standard Deviation 2* Std. Dev. Observed Minimum Observed Maximum -CI =Mean - 2*Std. Dev. +CI =Mean + 2*Std. Dev. Volume - 1% (L) Volume + 1% (L) 10 - 0.1 = 9.9 100 - 1 = 99 500 - 5=495 10 + 0.1= 10.1 100 + 1 = 101 500 + 5=505 P20 P200 P1000

Performance OK? (Y/N) Calculate the Mean. Calculate the Standard Deviation. Multiply the standard deviation by 2 to get a 95% confidence level. Determine if your observed upper and lower confidence (Mean + or - the Standard Deviation *2) is within the 1% error range. If it is, then enter "OK" for Performance, otherwise enter "Need Calibration ".

Exercise 2 Open the Lab1 Populations sheet in Excel (2nd tab). There are three sets of data, a Population data set, a W sample, and a M sample. First calculate the Mean, Variance, Standard Deviation, and n for each sample set (using the functions found in Excel. Your instructor will demonstrate how to do this. Use the CountIf function to count the number of values in each of the bins. This will give you data for the frequency distribution columns and will be used to make a graph. Plot the frequency of each of the three datasets on the same graph. Print your results and turn them in. (OR show your instructor your results and have him sign this sheet.)