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JF766609 1133 bp DNA linear BCT 28-FEB-2012 Pseudomonas putida strain NBRIC19 dihydrolipoamide succinyltransferase gene, partial cds. ACCESSION JF766609 VERSION JF766609.1 GI:347015284 KEYWORDS . SOURCE Pseudomonas putida ORGANISM Pseudomonas putida Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 1133) AUTHORS Mishra,S., Mishra,A., Chauhan,P.S., Mishra,S.K., Kumari,M., Niranjan,A. and Nautiyal,C.S. TITLE Pseudomonas putida NBRIC19 dihydrolipoamide succinyltransferase (SucB) gene controls degradation of toxic allelochemicals produced by Parthenium hysterophorus JOURNAL J. Appl. Microbiol. (2012) In press PUBMED 22324517 REMARK Publication Status: Available-Online prior to print REFERENCE 2 (bases 1 to 1133) AUTHORS Mishra,S. and Nautiyal,C.S. TITLE Direct Submission JOURNAL Submitted (01-APR-2011) Division of Plant Microbe Interactions, NBRI, Rana Pratap Marg, Lucknow, Uttar Pradesh 226001, India FEATURES Location/Qualifiers source 1..1133 /organism="Pseudomonas putida" /mol_type="genomic DNA" /strain="NBRIC19" /isolation_source="rhizosphere soil" /db_xref="taxon:303" CDS <1..804 /function="catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2" /note="part of oxoglutarate dehydrogenase complex" /codon_start=1 /transl_table=11 /product="dihydrolipoamide succinyltransferase" /protein_id="AEO72150.1" /db_xref="GI:347015285" /translation="EGLVAASHKKSAPAAAAKPAAAAATAPVVVAAGDRTEKRVPMTR LRAKIAERLVEAQSSMAMLTTFNEVDMTEVMALRSKYKDLFEKTHNGVRLGFMSFFVK AATEALKRFPAVNASIDGNDIVYHGYADVGVAVSSDRGLVVPVLRNAESMSLAEIENG IATFGKKARDGKLAIEEMTGGTFTITNGGTFGSMMSTPIVNPPQAAILGMHNIIQRPM AINGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNLLEDPSRLLLDI" ORIGIN 1 gaaggattgg tcgctgcgtc gcacaagaag tcggcgccag ctgcagctgc caagcctgct 61 gccgctgccg ctaccgcccc ggttgtcgtt gccgctggcg accgcaccga gaagcgtgtg 121 ccgatgaccc gcctgcgcgc caagattgcc gagcgtctgg tcgaagccca gtcgagcatg 181 gccatgctga ccaccttcaa cgaagtcgac atgaccgagg tcatggccct gcgttcgaag 241 tacaaggacc tgttcgagaa gacccacaac ggcgtacgcc tgggcttcat gtcgttcttc 301 gtcaaggccg ccaccgaagc cctgaagcgc ttcccggctg tcaacgcctc gatcgacggc 361 aacgacatcg tctaccacgg ctacgccgac gtcggcgttg ccgtgtccag cgaccgtggc 421 ctggtggtgc cggtactgcg taacgccgag tcgatgagcc tggcggaaat cgagaacggc 481 atcgccacct tcggcaaaaa agcccgtgac ggcaagctgg ccatcgaaga gatgaccggc 541 ggcactttca ccatcaccaa cggtggtacc ttcggttcga tgatgtcgac cccgatcgtc 601 aacccgccgc aggccgccat tctcggcatg cacaacatca tccagcgccc gatggccatc 661 aatggccagg tagtgattcg cccgatgatg tacctggcgc tgtcgtacga tcaccgcctg 721 atcgacggca aggaagcggt aaccttcctg gtcaccatca agaacctgct ggaagatccg 781 tctcgcctgc tgctggacat ctaaccctct ggctgcgtac cgtgccggcc ctggcttcgc 841 aggaagccat gccgccgcgg ttcgcggctt gtagcttgta gctgaataag gaatcttttt 901 atgaccagaa attcgacgta gtggtgatgg tgcagtctgc gctattgtgc tgcatcaagc 961 cgcacaaact gtctgaagac tgcttgtatt cgagaagtac acccgaccgc cgaaggcaac 1021 ctgactctgg gcggtacctt gcctggacgt tagtgcatcc ttcagcgtgc tgacacagct 1081 ctctgaagtc aggaatcaga gttccaggtc cagacatctc cccacatgtg cga //
5cctgaagcgc ttcccggctg tcaacgcctc gatcgacggc aacgacatcg tctaccacgg ctacgccgac gtcggcgttg ccgtgtccag cgaccgtggc ctggtggtgc cggtactgcg taacgccgag tcgatgagcc tggcggaaat cgagaacggc atcgccacct tcggcaaaaa agcccgtgac ggcaagctgg ccatcgaaga gatgaccggc ggcactttca ccatcaccaa cggtggtacc ttcggttcga tgatgtcgac cccgatcgtc aacccgccgc aggccgccat tctcggcatg cacaacatca tccagcgccc gatggccatc aatggccagg tagtgattcg cccgatgatg tacctggcgc tgtcgtacga tcaccgcctg atcgacggca aggaagcggt aaccttcctg gtcaccatca agaacctgct ggaagatccg tctcgcctgc tgctggacat ctaaccctct ggctgcgtac cgtgccggcc ctggcttcgc aggaagccat gccgccgcgg ttcgcggctt 3
Primer Forward 5 cctgaagcgc ttcccggctg 3 Tm = 4(g+c)+2(a+t) = 4(6+8)+2(2+4) = 72 0C A = Tm5 = 72-5 = 67 0C Primer Revers 5 aagccgcgaa ccgcggcggc 3 Tm = 4(g+c)+2(a+t) = 4(8+8)+2(4+0) = 72 0C A = Tm5 = 72-5 = 67 0C Urutan Primer 5ke 3 F R cctgaagcgc ttcccggctg aagccgcgaa ccgcggcggc Panjang Primer 20 20 Anneal (0C) 67 PCR Product 550
5cctgaagcgc ttcccggctg tcaacgcctc gatcgacggc aacgacatcg tctaccacgg ctacgccgac gtcggcgttg ccgtgtccag cgaccgtggc ctggtggtgc cggtactgcg taacgccgag tcgatgagcc tggcggaaat cgagaacggc atcgccacct tcggcaaaaa agcccgtgac ggcaagctgg ccatcgaaga gatgaccggc ggcactttca ccatcaccaa cggtggtacc ttcggttcga tgatgtcgac cccgatcgtc aacccgccgc aggccgccat tctcggcatg cacaacatca tccagcgccc gatggccatc aatggccagg tagtgattcg cccgatgatg tacctggcgc tgtcgtacga tcaccgcctg atcgacggca aggaagcggt aaccttcctg gtcaccatca agaacctgct ggaagatccg tctcgcctgc tgctggacat ctaaccctct ggctgcgtac cgtgccggcc ctggcttcgc aggaagccat gccgccgcgg ttcgcggctt 3
Primer Forward 5 ggacttcgcg aagggccgac 3 Tm = 4(g+c)+2(a+t) = 4(8+6)+2(4+2) = 72 0C A = Tm5 = 72-5 = 67 0C Primer Revers 5 ttcggcgctt ggcgccgccg 3 Tm = 4(g+c)+2(a+t) = 4(8+8)+2(0+4) = 72 0C A = Tm5 = 72-5 = 67 0C Urutan Primer 5ke 3 F R ggacttcgcg aagggccgac ttcggcgctt ggcgccgccg Panjang Primer 20 20 Anneal (0C) 67 PCR Product 550
LOCUS DEFINITION
JQ236807 1457 bp DNA linear BCT 28-FEB-2012 Pseudomonas fluorescens strain hswx163 16S ribosomal RNA gene, partial sequence. ACCESSION JQ236807 VERSION JQ236807.1 GI:377830128 KEYWORDS . SOURCE Pseudomonas fluorescens ORGANISM Pseudomonas fluorescens Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 1457) AUTHORS Wei,S. TITLE Direct Submission JOURNAL Submitted (04-DEC-2011) College of Life Science, Heng Shui University, Heng Shui, He Bei 053000, China FEATURES Location/Qualifiers source 1..1457 /organism="Pseudomonas fluorescens" /mol_type="genomic DNA" /strain="hswx163" /db_xref="taxon:294" /PCR_primers="fwd_name: 27f, fwd_seq: agagtttgatcctggctcag, rev_name: 1492r, rev_seq: ggttaccttgttacgactt" rRNA <1..>1457 /product="16S ribosomal RNA" ORIGIN 1 ttacaccgtc agaaaaaaat tttttatgcg cgctaccatg caagtcgagc ggtagagaga 61 agcttgcttc tcttgagagc ggcggacggg tgagtaatgc ctaggaatct gcctggtagt 121 gggggataac gttcggaaac ggacgctaat accgcatacg tcctacggga gaaagcaggg 181 gaccttcggg ccttgcgcta tcagatgagc ctaggtcgga ttagctagtt ggtggggtaa 241 tggctcacca aggcgacgat ccgtaactgg tctgagagga tgatcagtca cactggaact 301 gagacacggt ccagactcct acgggaggca gcagtgggga atattggaca atgggcgaaa 361 gcctgatcca gccatgccgc gtgtgtgaag aaggtcttcg gattgtaaag cactttaagt 421 tgggaggaag ggttgtagat taatactctg caattttgac gttaccgaca gaataagcac 481 cggctaactc tgtgccagca gccgcggtaa tacagagggt gcaagcgtta atcggaatta 541 ctgggcgtaa agcgcgcgta ggtggttagt taagttggat gtgaaatccc cgggctcaac 601 ctgggaactg cattcaaaac tgactgacta gagtatggta gagggtggtg gaatttcctg 661 tgtagcggtg aaatgcgtag atataggaag gaacaccagt ggcgaaggcg accacctgga 721 ctgatactga cactgaggtg cgaaagcgtg gggagcaaac aggattagat accctggtag 781 tccacgccgt aaacgatgtc aactagccgt tgggagcctt gagctcttag tggcgcagct 841 aacgcattaa gttgaccgcc tggggagtac ggccgcaagg ttaaaactca aatgaattga 901 cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg aagaacctta 961 ccaggccttg acatccaatg aactttctag agatagattg gtgccttcgg gaacattgag 1021 acaggtgctg catggctgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgtaac 1081 gagcgcaacc cttgtcctta gttaccagca cgtaatggtg ggcactctaa ggagactgcc 1141 ggtgacaaac cggaggaagg tggggatgac gtcaagtcat catggccctt acggcctggg 1201 ctacacacgt gctacaatgg tcggtacaga gggttgccaa gccgcgaggt ggagctaatc 1261 ccacaaaacc gatcgtagtc cggatcgcag tctgcaactc gactgcgtga agtcggaatc 1321 gctagtaatc gcgaatcaga atgtcgcggt gaatacgttc ccgggccttg tacacaccgc 1381 ccgtcacacc atgggagtgg gttgcaccag aagtagctag tctaacgtcg gagacgtacc 1441 atcggtgatt gccatcg //
LOCUS
JQ219349
591 bp DNA
DEFINITION Saccharomyces cerevisiae strain BJ103 26S ribosomal RNA gene, partial sequence. ACCESSION JQ219349 VERSION JQ219349.1 GI:377823382 .
KEYWORDS SOURCE
ORGANISM Saccharomyces cerevisiae Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. REFERENCE 1 (bases 1 to 591) AUTHORS Chen,R., Jiang,Y.M. and Wei,S.C. TITLE Species diversity of yeasts in apple orchard
JOURNAL Unpublished REFERENCE 2 (bases 1 to 591) AUTHORS Chen,R., Jiang,Y.M. and Wei,S.C. TITLE Direct Submission
JOURNAL Submitted (01-DEC-2011) College of Horticulture Science and Engineering, Shandong Agricultural University, No. 61 Daizong Street, Tai'an, Shandong 271018, China FEATURES source Location/Qualifiers 1..591 /organism="Saccharomyces cerevisiae" /mol_type="genomic DNA" /strain="BJ103" /db_xref="taxon:4932" rRNA <1..>591 /product="26S ribosomal RNA" /note="D1/D2 domain" ORIGIN 1 aaaccaaccg ggattgcctt agtaacggcg agtgaagcgg caaaagctca aatttgaaat 61 ctggtacctt cggtgcccga gttgtaattt ggagagggca actttggggc cgttccttgt
121 ctatgttcct tggaacagga cgtcatagag ggtgagaatc ccgtgtggcg aggagtgcgg 181 ttctttgtaa agtgccttcg aagagtcgag ttgtttggga atgcagctct aagtgggtgg 241 taaattccat ctaaagctaa atattggcga gagaccgata gcgaacaagt acagtgatgg 301 aaagatgaaa agaactttga aaagagagtg aaaaagtacg tgaaattgtt gaaagggaag 361 ggcatttgat cagacatggt gttttgtgcc ctctgctcct tgtgggtagg ggaatctcgc 421 atttcactgg gccagcatca gttttggtgg caggataaat ccataggaat gtagcttgcc 481 tcggtaagta ttatagcctg tgggaatact gccagctggg actgaggact gcgacgtaag 541 tcaaggatgc tggcataatg gttatatgcc gcccgtcttg aaacacggac c //
LOCUS DEFINITION
JF317686 1347 bp mRNA linear PLN 11-FEB-2012 Rhizopus oryzae strain DSM 63539 chitin deacetylase mRNA, partial cds. ACCESSION JF317686 VERSION JF317686.1 GI:330689932 KEYWORDS . SOURCE Rhizopus oryzae ORGANISM Rhizopus oryzae Eukaryota; Fungi; Fungi incertae sedis; Early diverging fungal lineages; Mucoromycotina; Mucorales; Mucoraceae; Rhizopus. REFERENCE 1 (bases 1 to 1347) AUTHORS Kulmann,C., Lange,M. and Warrelmann,J. TITLE Functional and phylogenetic characterization of a newly discovered chitin deacetylase from Rhizopus oryzae JOURNAL Unpublished REFERENCE 2 (bases 1 to 1347) AUTHORS Kulmann,C., Lange,M. and Warrelmann,J. TITLE Direct Submission JOURNAL Submitted (10-FEB-2011) Department of Biology, University of Bremen, Center for Environmental Research and Sustainable Technology, Leobener Strasse, Bremen 28359, Germany FEATURES Location/Qualifiers source 1..1347 /organism="Rhizopus oryzae" /mol_type="mRNA" /strain="DSM 63539" /db_xref="taxon:64495" CDS 1..>1347 /EC_number="3.5.1.41" /function="catalyzes removal of acetate from chitin and chitosan" /codon_start=1 /product="chitin deacetylase" /protein_id="AEC33271.1" /db_xref="GI:330689933" /translation="MYIKTSALAIAILHVAGLAEAAKSSESKSSSHSKSNSTLSKPQD YWKNFKSLVDPNNITIPAIAQTTSLDPSDECKYYEPPSNFVYNAKEWPNLWETATSNG MTKTAEFQALYKSIDWTKAPKIAVRKKTSDGGVDMTSYPDSDPDCWWSSSTCTKPKHK DVNEDIYACPEPETWGLTYDDGPNCSHNAFYDYLEQNKIKASMFYIGSNVVNWPYGAQ RGVKAGHHIADHTWSHQLMTTLSNEEVLAELYYTQKAIKMVTGVTPLHWRPAFGDVDD RVRWIATQLNLTTILWNLDTDDWAAGSSKTVDEVKATYDAYVQMGSNGTFANSGQIVL THEIDNTTMSLAVEYLPKITAAYKNVVDVATCMNITYPYQEHTVSFAPFGSSANSSST SASSDAAPAASSAATADAPGTTTISLAANKAEVPVSAGIQINPNSLIVAAFAAAYFF" sig_peptide 1..66 /note="extracellular secretion signal" misc_feature 106..117 /note="Region: N-glycosylation motif" misc_feature 502..1074 /note="Region: chitin deacetylase domain" misc_feature 502..870 /note="Region: polysaccharide deacetylase domain" ORIGIN 1 atgtacatta aaacttctgc ccttgcaatt gccatcttgc acgttgccgg attagccgaa 61 gctgccaaga gctctgagag caagtcatca tcccattcca agtccaacag cactctttca 121 aagcctcagg attattggaa gaacttcaaa tccttggttg atcccaacaa catcaccatc 181 cctgctattg cccaaaccac ttccttagat ccttctgatg aatgtaaata ctacgagcct 241 ccttccaact ttgtctacaa tgccaaggag tggcccaacc tttgggaaac tgccacttcc 301 aacggtatga ctaagactgc cgagttccaa gctctttaca agtcaattga ttggaccaag 361 gctcccaaga ttgccgttcg taagaagacc tccgatggtg gcgttgacat gaccagttat 421 ccagacagtg accctgactg ttggtggtca tcctccacct gtaccaagcc caagcataag 481 gacgttaatg aagatatcta cgcttgtccc gaacctgaaa cttggggtct tacttacgat 541 gatggaccta actgttctca caatgctttc tatgactacc ttgagcaaaa caagatcaag 601 gctagtatgt tctacattgg ttccaacgtc gtcaactggc cctacggtgc tcaacgtggt 661 gtcaaggctg gccatcatat tgctgatcac acctggtctc atcaattgat gaccaccttg 721 agcaatgaag aagtccttgc cgaactctac tatactcaaa aagctatcaa gatggtcact 781 ggtgttactc ctctccactg gcgtcctgct ttcggtgatg tcgacgatcg tgttcgttgg
LOCUS DEFINITION
FN313541 560 bp DNA linear PLN 22-APR-2009 Aspergillus niger var. tubingensis intergenic spacer, IGS, isolate PPL 2009-2. ACCESSION FN313541 VERSION FN313541.1 GI:227430105 KEYWORDS . SOURCE Aspergillus niger var. tubingensis ORGANISM Aspergillus niger var. tubingensis Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; Aspergillus. REFERENCE 1 AUTHORS Bardas,G.A., Lotos,L.C. and Tzelepis,G.D. TITLE First report of Botrytis cinerea, Penicillium glabrum and Aspergilus niger sub sp. tubigensis on pomegranate in Greece JOURNAL Unpublished REFERENCE 2 (bases 1 to 560) AUTHORS Tzelepis,G.D. TITLE Direct Submission JOURNAL Submitted (06-APR-2009) Tzelepis G.D., Paraskevopoulou 44, Kalamaria, Thessaloniki, 55133, GREECE FEATURES Location/Qualifiers source 1..560 /organism="Aspergillus niger var. tubingensis" /mol_type="genomic DNA" /variety="tubingensis" /isolate="PPL 2009-2" /isolation_source="pomegranate fruit" /db_xref="taxon:640706" /country="Greece" /identified_by="G.D. Tzelepis, L. Lotos and G.A. Bardas" misc_feature 1..560 /note="intergenic spacer, IGS" ORIGIN 1 aaggatcatt accgagtgcg ggtcctttgg gcccaacctc ccatccgtgt ctattatacc 61 ctgttgcttc ggcgggcccg ccgcttgtcg gccgccgggg gggcgccttt gccccccggg 121 cccgtgcccg ccggagaccc caacacgaac actgtctgaa agcgtgcagt ctgagttgat 181 tgaatgcaat cagttaaaac tttcaacaat ggatctcttg gttccggcat cgatgaagaa 241 cgcagcgaaa tgcgataact aatgtgaatt gcagaattca gtgaatcatc gagtctttga 301 acgcacattg cgccccctgg tattccgggg ggcatgcctg tccgagcgtc attgctgccc 361 tcaagcccgg cttgtgtgtt gggtcgccgt ccccctctcc ggggggacgg gcccgaaagg 421 cagcggcggc accgcgtccg atcctcgagc gtatggggct ttgtcacatg ctctgtagga 481 ttggccggcg cctgccgacg ttttccaacc attttttcca ggttgacctc ggatcaggta 541 gggatacccg ctgaacttaa //
tersebut juga terdapat pada produk-produk kacang dan makanan lain. Jamur tersebut menghasilkan racun aflatoksin. Biji-bijian lain kerusakannya disebabkan oleh Claviceps purpurea, dalam biji gandum, sorgum dan barley menyebabkan ergot disease. Racun jamur menyebabkan kejang-kejang dan halusinasi (ESR). (Ditrejemahkan dari www.microbiologyprocedure.com) Diposkan oleh Ekaning Siti Rahayu, S.TP., MP. dan Siti Asmaniyah Mardiyani, SP., MP. di 16:00
http://hasanahcenter.blogspot.com/2010/05/mikrobiologi-dan-kerusakan-makanan.html