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Introduction Types of incompatibility Genetic basis of Self-incompatibility Level of its occurrence Physiology and Biochemistry Biological significance Methods to overcome Incompatibility
# Heterogenic. Controlled by more than one gene. Linskens(1975) #Basis of new races & prevents free Cross-Pollination
1. INTERSPECIFIC
2.SELF INCOMPATIBILITY
#Inability of a plant producing functional male and female gametes. #TWO TYPES- 1.Heteromorphic and 2.Homomorphic
*Heteromorphic : -Features
Characterised by occurrence of two (distyly) or three (tristyl) morphologically distinct mating type. -Due to inhibition of growth of pollen.
*Homomorphic :-
Indistinguishable type. Categorized into:(i)Gametophytic self-incompatibility(GSI): Determined by genotype of male gametophyte itself. Eg.Graminaceae, Liliaceae, Solanaceae &Trifolium. (ii)Sporophytic self-incompability(SSI): Controlled by genotype of the sporophytic tissue of the plant from which the pollen is derived. Eg,. Compositae & Cruciferae.
plant showing gametophytic incompatibility. A- No Pollen from S1S2 plant is able to effect fertilization. B- Only S3 plant succeded in fertilizing ovules resulting into 50% fertilization.
the style.
(b)In the style- Pollen tube fail to grow through the full length of
BUD-POLLINATION :
STUB-POLLINATION :
INTRA-OVARIAN POLLINATION :
In this technique, the ovary surface is sterilised with ethanol and two punctures are made in the wall- one for introducing the pollen suspension and the other to permit the escape of of air in the ovarian cavity(Kanta et al.,1962). Variable seeds following intra-ovarian pollination have obtained in several members of Papaveraceae such as Argemone mexicana, A.
50 C) are known to reduce the self-incompability reaction in certain plants. e.g,. Lilium longiflorum (Hooper et al 1967).
: In 1969 a new approach was proposed to raised such hybrid which could not be produced through the conventional method of hybridisation because of sexual incompatibility. This technique involves fusion of isolated protoplast of somatic cell and is described as parasexual hybridisation. This involves the following step :-
(a)Isolation of protoplasts. (b)Fusion of isolated protoplasts. (c)Cultured of hybrid protoplasts to regenerate whole plant.