INTESTINE

Duodenum Jejunum Ileum Appendix Large intrestine

DUODENUM
MUCOSA- villi,intervillus space, epithelium, basement membrane lamina propria muscularis mucosa Submucosa Muscularis externa Serosa

Villi (longitudinal section)

Fold Apex-- narrow

Basebroad
Intervillus space which continue as intestinal gland Lining epithelium with brush border

Lie on basement membrane

Villi (longitudinal section) contents

lamina propria
Intestinal glands Fine connective tissue Reticular cells Diffuse lymphatic tissue Lymphatic nodule in deep part (may be seen) Muscle fibers

Villi (longitudinal section) contents

lamina propria
Central lacteal- a small dialated lymphatic vessel line with endothelium Blood vessel- arteriole One or more venules Numerous Capillaries

Villi

Expansion of the absorptive mucosal surface

Schematic-Villi & crypts of small intestine

Intestinal mucosa

Note prominent villi & short intestinal glands opening between the bases of villi

Duodenum

Duodenum - LS

Note submucosal Brunners glands

L.S. Duodenum

Duodenum

Duodenum PAS/haematoxylin

Duodenum -Alcian blue

Intestinal villi & crypts

Paneth cell in base of crypt of Lieberkuhn Phloxine-tartrazine stain

Intestinal villi

Ly = lymphocytes P = plasma cell M = Smooth muscle fibres C = capillaries

Intestinal villi

PAS/Iron haematoxylin/Orange G

Intestinal villi -TS

L = lacteal Ly = lymphocytes

Intestinal villi & crypts

Enzyme histochemical method for Alkaline phosphatase

Carmine perfusion method to demonstrate the blood supply

Microcirculation of intestinal villus

LS of a Jejunal villus

Lacteal

Intestinal villi to demonstrate chylomicrons

Sudan black stain

Intestinal villi - tip

Toluidine blue SB=Surface brush border

EM - Jejunal villi

Foliate & finger- like

EM-Duodenal Villi & underlying crypts

Intestinal villi & crypts

Scanning EM of intestinal mucosa viewed from the lumen after removal of the epithelium

Foliate villi

Openings of recesses occupied by the glands

Intestinal microvilli & glycocalyx coat

Oligosaccharide chains emerging from the outer leaflet of plasma membrane

EM T.S. of microvilli

Note actin filaments in the core

Bundles of actin filaments extending from microvilli

Goblet cell & surrounding enterocytes

Enterocytes of small intestine - EM

Enterocytes of small intestine - EM

Enterocytes of small intestine - EM

Base of crypt of Lieberkuhn

Portion of Paneth cell

Small intestine

Lymphoid follicle

Scanning EM Epithelium over lymphoid follicle

M cells

Schematic representation of IgA secretion

Subepithelial fibroblasts fenestrated

Schematic representation of Ileum

Ileum

Note Peyers patches

Ileum

Lipid droplets in intestinal lining cell

Schematic ultrastructure of intestinal absorptive cell

Fasting state

Lipid rich meal

Schematic representation of chylomicron formation

Chylomicrons in intercellular space

Ileocaecal junction

T.S. of Appendix

Appendix -T.S.

Appendix -T.S.

Appendix

Note lymphoid follicle & lamina propria

Large intestine

L.S. of Large intestine

Scanning EM of descending colon

Large intestine note crypts & goblet cells

L.S.

T.S.

Crypt from rectal mucosa

Large intestine

TS Alcian blue/Van Gieson

H/E

Scanning EM of submucous nerve plexus

Intestinal myenteric plexus

Multipolar neuron in myenteric plexus

Recto-anal junction