Scribd Logo
Încărcați

Bine ați venit la Scribd!

  • Practical Biochemistry - Revision of Biochemistry

    Încărcat de

    Adlina Tajuddin
    387 vizualizări17 pagini
    Titration is a process in which a quantitative determination of the substance occurs in a stepwise reaction up to the end point. Alkaline tide is the presence of an alkaline urine that often follows the ingestion of a meal due to the arrival of excess bicarbonates to blood during the process of HCl secretion by the stomach.

    Descriere originală:

    Drepturi de autor

    © Attribution Non-Commercial (BY-NC)

    Formate disponibile

    PPTX, PDF, TXT sau citiți online pe Scribd

    Vi se pare util acest document?

    Este necorespunzător acest conținut?

    Raportați acest document
    Titration is a process in which a quantitative determination of the substance occurs in a stepwise reaction up to the end point. Alkaline tide is the presence of an alkaline urine that often follows the ingestion of a meal due to the arrival of excess bicarbonates to blood during the process of HCl secretion by the stomach.

    Drepturi de autor:

    Attribution Non-Commercial (BY-NC)
    Descărcați ca PPTX, PDF, TXT sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    387 vizualizări17 pagini

    Practical Biochemistry - Revision of Biochemistry

    Încărcat de

    Adlina Tajuddin
    Titration is a process in which a quantitative determination of the substance occurs in a stepwise reaction up to the end point. Alkaline tide is the presence of an alkaline urine that often follows the ingestion of a meal due to the arrival of excess bicarbonates to blood during the process of HCl secretion by the stomach.

    Drepturi de autor:

    Attribution Non-Commercial (BY-NC)
    Descărcați ca PPTX, PDF, TXT sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    din 17

    REVISION OF BIOCHEMISTRY (GIT module)

    (This power point is not originally from section of practical class. It is made to clarify all points that are emphasized by professors during revision class) *do not depend on this power point only. Read
    the topics inside the book is recommended.

    ESTIMATION OF FREE AND TOTAL ACIDITY IN A GASTRIC JUICE SAMPLE

    GASTRIC JUICE TITRATION

    X1 Definition of end point No of mls of 0.1 N NaOH in neutralize free acid in 5mL gastric juice Free acidity (free HCl) Topfer 3 No of mls of 0.1 N NaOH in neutralize free acid in 100mL gastric juice

    X2 No of mls of 0.1 N NaOH in neutralize total acid in 5mL gastric juice Total Acidity (Free HCl + Combined HCl (end point)+ organic acid) Phenolphthalein 8 No of mls of 0.1 N NaOH in neutralize total acid in 100mL gastric juice

    Acidity: Indicator pH

    X1 x 100/5 =X1 x 20 =0.1N NaOH %

    X2 x 100/5 =X2 x 20 =0.1N NaOH %

    CHANGES OF pH
    pH = 1 pH gastric juice pH =3 (free acidity) Topfer= salmon pink

    pH=8 (total acidity) Phenolphthalein (pink)

    Questions suggested
    1. Definition of titration? ans: It is a process in which a quantitative determination of the substance occurs in a stepwise reaction up to the end point. 2. Types of titration ? (details in the book pg 102 -103) ans: 1. Acid-base titration 2. Precipitation titration 3. Oxidation-reduction titration

    3. Definition of end point? ans: The point in a titration where physical change ( in the solution being titrated) manifests itself
    4. Definition of alkaline tide? ans: The presence of an alkaline urine that often follows the ingestion of a meal due to the arrival of excess bicarbonates to blood during the process of HCl secretion by the stomach.

    5. Fractional test meal?

    a 15-30minutes after meal


    Acidity falls to a lower level Why?
    Dilution of gastric juice with saliva and tea Neutralizing of gastric juice by:- NaHCO3 of saliva - protein of meal - mucin in the stomach

    b 30-90 minutes after meal


    Acidity rises again and reaches maximum value after about 90minutes. Why?
    Stimulation of the gastric glands by the meal

    C- 90-150 minutes after meal


    After 120min, acidity starts to decline gradually until the fasting level is nearly reached at the end of about 150min. Why?
    Gastric secretion gets diminished Gastric contents are evacuated to the duodenum Gastric contents are neutralized by duodenal regurgitation.

    CAUSES OF ABNORMAL CURVES


    Hyperacidity (hyperchlorhydria) -why? duodenal ulcer - gall bladder disease Hypoacidity (hypochlorhydria)
    Why? gastric carcinoma

    Anacidity ( achlorhydria)
    Why? prenicious anemia

    SERUM AMYLASE

    SITE OF PRODUCTION
    Pancrease (the most) Salivary glands

    CAUSES OF INCREASING SERUM AMYLASE?


    Pancreas- pancreatitis - carcinoma Abdomen -gastritis -bile ducts problem (eg; obstruction) Salivary salivary prob (eg. Inflammation of salivary glands) Genitourinary problem

    PRINCIPLE
    Starch (sample) amylo dextrin (residual starch)
    1-4 glucosidase (inside serum)

    pale blue black colour


    Iodine

    The higher the enzymatic activity, the lower the intensity of colour, the lower the absorbance

    Calculation
    AB AS x 1480 AB = .u/L

    THANK YOU AND ALL THE BEST ACErs!!


    Do not despair, Allah is always with us ^_^

    S-ar putea să vă placă și