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Schematic drawing of a bacterium with its plasmids. (1) Chromosomal DNA. (2) Plasmids
RB Shahi
Capable of autonomous replication i.e. self- replicating A plasmid is a DNA molecule separate from the chromosomal DNA i.e.. Extra chromosomal(replicons) That are commonly present in bacteria, archaea, and yeasts Typically circular and double-stranded but Linear plasmids have been found in a variety of bacteria,e.g. Streptomyces sp. and Borrelia burgdorferi Often carry genetic information that is useful only under atypical conditions, and are sometimes lost from the cell (in non-selective environment)
heredity and suggests the term "Plasmid" for all extrachromosomal hereditary determinants.
colicine (plasmids) (large toxin proteins (50-70kD) ) behave as genetic factors independent of the chromosome.
term "Episome" to describe genetic elements such as F factor, colicine, and phage lambda, which can exist both in association with the chromosome and independent of it.
1) "Supercoiled" (or "Covalently Closed-Circular") DNA is fully intact with both strands uncut.
Nicked DNAs
Linear DNA
Nicked Open Circular (slowest) Linear Relaxed Circular Supercoiled Denatured Supercoiled (fastest)
Classification of Plasmids
Based upon the number of copies per cell, plasmids are classified into two types.
Stringent plasmids Relaxed plasmids
Based upon plasmid incompatibility: inability of two different plasmids to co exist in the same cell in the absence of selection pressure
Compatible Incompatible
Based upon the ability to take part in conjugation, plasmids are of two types.
Conjugative non conjugative
Host Range
Broad spectrum Narrow spectrum
Phenotypic effects
Fertility Bacteriocinogenic plasmid Resistance plasmid (R factors) Etc.
Stringent plasmids
These plasmids exist in small numbers, i.e. <100 copies/cell (limited number of copies per cell) Under the control of bacterial genome for replication and segregation Generally, conjunctive plasmids are mostly stringent plasmids.
Relaxed plasmids
These plasmids exist in large numbers, i.e., > 100 copies/cell.(multiple copies per Cell) not under the control of bacterial genome for replication and segregation Generally they are of low molecular weight most of them are of the non conjugative type.
Compatible
Have different replication control
Incompatible
Have same replication control
Conjugative
those which take part in conjugation They have tra genes which help in conjugation, e.g. F. Plasmids
non conjugative
those which does nittake part in conjugation They lack tra genes which help in conjugation, e.g. pBR 322
Broad spectrum
Replicate in many types of bacteria
RSF 1010(wide range of Gram-negative and Gram-positive bacteria-non conjugative) RP4(most Gram-negative bacteria-conjugation) etc
Narrow spectrum
Replicate in only one type of bacteria
pBR322 in E .coli
Maintenance of Plasmids
Two factors are important
One is their faithful replication at least once in each cell cycle: Regulated
1. by antisense RNA and 2. by binding of essential proteins to iterons
their accurate partition so that each daughter cell receives at least one copy
Rop=Repressor of Primer
Function of Plasmids
1) Antibiotic resistance 2) Antibiotic production 3) Degradation of aromatic compounds 4) Haemolysin production 5) Sugar fermentation 6) Enterotoxin production 7) Heavy-metal resistance 8) Bacteriocin production 9) Induction of plant tumours 10)Hydrogen sulphide production 11)Host-controlled restriction and modification
What are cryptic plasmids?