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History of DNA
History of DNA
Early scientists thought protein was the cells hereditary material because it was more complex than DNA Proteins were composed of 20 different amino acids in long polypeptide chains
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Transformation
Fred Griffith worked with virulent S and nonvirulent R strain Pneumoccocus bacteria He found that R strain could become virulent when it took in DNA from heat-killed S strain Study suggested that DNA was probably the genetic material
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Griffith Experiment
History of DNA
Chromosomes are made of both DNA and protein Experiments on bacteriophage viruses by Hershey & Chase proved that DNA was the cells genetic material
Chargaffs Rule
Adenine must pair with Thymine Guanine must pair with Cytosine The bases form weak hydrogen bonds
C
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DNA Structure
Rosalind Franklin took diffraction x-ray photographs of DNA crystals In the 1950s, Watson & Crick built the first model of DNA using Franklins x-rays
Rosalind Franklin
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DNA Structure
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DNA
Two strands coiled called a double helix Sides made of a pentose sugar Deoxyribose bonded to phosphate (PO4) groups by phosphodiester bonds Center made of nitrogen bases bonded together by weak hydrogen bonds
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Legs of ladder
Helix
Most DNA has a right-hand twist with 10 base pairs in a complete turn Left twisted DNA is called Z-DNA or southpaw DNA Hot spots occur where right and left twisted DNA meet producing mutations
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DNA
Stands for Deoxyribonucleic acid Made up of subunits called nucleotides Nucleotide made of: 1. Phosphate group 2. 5-carbon sugar 3. Nitrogenous base
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DNA Nucleotide
Phosphate Group O
5
O=P-O O
CH2 O N
C4
Sugar (deoxyribose)
C1
C3
C2
Pentose Sugar
Carbons are numbered clockwise 1 to 5 5
CH2
O C1
C4
Sugar (deoxyribose)
C3
C2
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DNA
O
3
P
5 4
O
O
G
2
C
1
4
3
P
5
T
O
A
O
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Antiparallel Strands
One strand of DNA goes from 5 to 3 (sugars) The other strand is opposite in direction going 3 to 5 (sugars)
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Nitrogenous Bases
Double ring PURINES Adenine (A) Guanine (G) Single ring PYRIMIDINES Thymine (T) Cytosine (C) T or C
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A or G
Base-Pairings
Purines only pair with Pyrimidines Three hydrogen bonds required to bond Guanine & Cytosine
3 H-bonds
C
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Bonding 1 The bases always pair up in the same way
Adenine
Thymine
Cytosine
Bonding 2
PO4
adenine thymine
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PO4
PO4 PO4
cytosine guanine
PO4 PO4
PO4
PO4
Pairing up
PO4
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PO4 PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
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A DOUBLE HELIX
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sugar-phosphate chain
replication Before a cell divides, the DNA strands unwind and separate
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PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
PO4
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PO4 PO4
PO4
PO4 PO4
PO4
PO4 PO4
PO4
PO4 PO4
PO4 PO4
PO4 PO4
PO4
PO4
PO4
PO4
PO4
PO4
Genetic code 1 The sequence of bases in DNA forms the Genetic Code
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A group of three bases (a triplet) controls the production of a particular amino acid in the cytoplasm of the cell
The different amino acids and the order in which they are joined up determines the sort of protein being produced
Genetic code 2
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This is a small, imaginary protein molecule showing how a sequence of 5 different amino acids could determine the shape and identity of the molecule
Ser-Cyst-Val-Gly-Ser-Cyst Ala Val
Val-Cyst-Ser-Ala-Ser-Cyst-Gly
Val- Cyst-Ala-Ala-Ser-Gly
Each amino acid (Serine, Cysteine, Valine, Glycine and Alanine) is coded for by a particular triplet of bases
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Adenine
Codes for
Valine
Codes for
Alanine
Triplet code This is known as the triplet code Each triplet codes for a specific amino acid
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CGA - CAA - CCA - CCA - GCT - GGG - GAG - CCA Ala Val Gly Gly Arg Pro Leu Gly
The amino acids are joined together in the correct sequence to make part of a protein Ala Val Gly Gly Arg Pro Leu Gly
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The structures and reactions in the cell determine what sort of a cell it is and what its function is So DNA exerts its control through the enzymes
Question:
If there is 30% Adenine, how much Cytosine is present?
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Answer:
There would be 20% Cytosine
Adenine (30%) = Thymine (30%) Guanine (20%) = Cytosine (20%) Therefore, 60% A-T and 40% C-G
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DNA Replication
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Replication Facts
DNA has to be copied before a cell divides DNA is copied during the S or synthesis phase of interphase New cells will need identical DNA strands
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S
DNA replication takes place in the S phase. phase
G1
interphase
G2
Mitosis
-prophase -metaphase -anaphase -telophase
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DNA Replication
Begins at Origins of Replication Two strands open forming Replication Forks (Y-shaped region) New strands grow at the forks
Replication Fork
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DNA Replication
As the 2 DNA strands open at the origin, Replication Bubbles form Prokaryotes (bacteria) have a single bubble Eukaryotic chromosomes have MANY bubbles
Bubbles Bubbles
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DNA Replication
Enzyme Helicase unwinds and separates the 2 DNA strands by breaking the weak hydrogen bonds Single-Strand Binding Proteins attach and keep the 2 DNA strands separated and untwisted
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DNA Replication
Enzyme Topoisomerase attaches to the 2 forks of the bubble to relieve stress on the DNA molecule as it separates
Enzyme Enzyme
DNA
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DNA Replication
Before new DNA strands can form, there must be RNA primers present to start the addition of new nucleotides Primase is the enzyme that synthesizes the RNA Primer DNA polymerase can then add the new nucleotides
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DNA Replication
DNA polymerase can only add nucleotides to the 3 end of the DNA This causes the NEW strand to be built in a 5 to 3 direction
5
RNA Primer
3 5
Nucleotide
DNA Polymerase
Direction of Replication
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O O=P-O O
CH2
O
N C4 C1
Nitrogenous base (A, G, C, or T)
Sugar (deoxyribose)
C3
C2
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P
5 4
O
O
G
2
C
1
4
3
P
5
T
O
A
O
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5
Nucleotides
DNA Polymerase
5
3 5 3
Lagging Strand
Leading Strand
3
5 3
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DNA Polymerase
RNA Primer
5
3
Lagging Strand
3
5
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Lagging Strand
5
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Replication of Strands
Replication Fork Point of Origin
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Question:
What would be the complementary DNA strand for the following DNA sequence?
DNA 5-CGTATG-3
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Answer:
DNA 5-CGTATG-3 DNA 3-GCATAC-5
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