HEAMATOLOGY SERVICE TRAINING

Blood

Blood Cell

Leukocyte

Erythrocyte

Platelet

Blood sample consists of two parts.

Version: 1.3, Update Date: 2008-2-1

Blood

Blood Cell

Leukocyte

Erythrocyte

Platelet

There are three kinds of blood cells.

Leukocyte Size: Amount: 30-400fl 9 4-10X10 /L

Erythrocyte 30-150fl 12 3.5-5.5X10 /L

Platelet 2-25fl 9 100-300X10 /L

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

Blood

Blood Cell

Leukocyte

Erythrocyte

Platelet

WBC
Defend the body against infectious disease and foreign materials

Granulocyte

Version: 1.3, Update Date: 2008-2-1

Blood

Blood Cell

Leukocyte

Erythrocyte

Platelet

Lymph

Baso

Mono

Eosino Neutro

Lymphocyte

Mid-sized Cell

Neutrophilic Granulocyte

Version: 1.3, Update Date: 2008-2-1

Blood

Blood Cell

Leukocyte

Erythrocyte

Platelet

The function of RBC is almost completed by hemoglobin (HGB).

Transport oxygen and carbon dioxide

Version: 1.3, Update Date: 2008-2-1

Blood

Blood Cell

Leukocyte

Erythrocyte

Platelet

Coagulation or clotting

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

Electrical Impedance Method

Volumetric Metering Colorimetric Method

Reagents

WBC, RBC and PLT are counted and sorted by the Electrical Impedance Method, just Coulter Principle. This method is based on the measurement of changes in electrical impedance produced by a particle passing through an aperture.

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

1.2 Principle
Electrical Impedance Method

Volumetric Metering Colorimetric Method

Reagents

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

1.2 Principle
Electrical Impedance Method

Volumetric Metering Colorimetric Method

Reagents

The amplitude of each pulse is proportional to the size of each blood cell.
Version: 1.3, Update Date: 2008-2-1

Electrical Impedance Method

Pulse Graph

Volumetric Metering Colorimetric Method

Reagents

1blood cell of Same size, go through the aperture by constant speed.

2blood cell of Same size, go through the aperture by variable speed.

3blood cell of different size, go through the aperture by variable speed.

Version: 1.3, Update Date: 2008-2-1

1.2 Principle
Electrical Impedance Method

Volumetric Metering Colorimetric Method

Reagents

Version: 1.3, Update Date: 2008-2-1

Electrical Impedance Method

Volumetric Metering Colorimetric Method

Reagents

From Pulse Graph to Histogram

Version: 1.3, Update Date: 2008-2-1

1.2 Principle
Electrical Impedance Method

Volumetric Metering Colorimetric Method

Reagents

From Pulse Graph to Histogram

Version: 1.3, Update Date: 2008-2-1

Electrical Impedance Method

Volumetric Metering

Colorimetric Method

Reagents

This analyzer uses a volumetric metering unit to control the count cycle and to ensure that a precise volume of sample is analyzed.
Upper sensor Upper sensor

Lower sensor

Lower sensor

1 Empty when start

2 Liquid surface fall down through the volumeritc tube

Upper sensor

Upper sensor

Lower sensor

Lower sensor

3 Counting start when the liquid1.3, surface pass the upper Version: Update Date: 2008-2-1 sensor.

4 Counting end when the liquid surface pass the lower sensor

Electrical Impedance Method

Volumetric Metering Colorimetric Method

Reagents

HGB is determined by the colorimetric method. Lyse will release HGB when RBC was broken down and react with HGB to generate a mixture for HGB measurement. The mixture has the biggest absorbance at 540nm.

I0

It

LAMBER BEER LAWConsantration is directly praportional to Abs.

Version: 1.3, Update Date: 2008-2-1

Electrical Impedance Method

Volumetric Metering Colorimetric Method

Reagents

Light source

Absorbing container

Detector

Signal processing Display

LED light

Bath

Photo sensor

Version: 1.3, Update Date: 2008-2-1

Electrical Impedance Method

Volumetric Metering Colorimetric Method

Reagents

Reagents are one part of the system. Lyse Break down RBC; Release HGB and react with HGB to generate a mixture for HGB measurement; Help the differential of WBC. Diluent Dilute the blood sample; Provide a conductive environment for counting and an environment which is similar to the plasma to maintain the size of blood cell; Clean the tube and bath.

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

Electrical Impedance Method

Volumetric Metering Colorimetric Method

Reagents

Rinse Clean the bath and metering tube. E-Z cleanser An enzyme-based cleaning solution used to clean the tube and bath.

Probe cleanser An alkaline cleaning solution used to clean the tube and bath.

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

Fluctuating
Discriminator 1 30~85fL

WBC

2, 3 85~125fL

Fixed Discriminator
4 400fL

[fL]

Lymph

Mid
(BasoMonoEosino)

Neutro

Version: 1.3, Update Date: 2008-2-1

No differential result

No differential result because the WBC histogram is overnarrowly compressed.

Version: 1.3, Update Date: 2008-2-1

No differential result

No differential result because WBC count result is less than a certain value (WBC < 0.5)

Version: 1.3, Update Date: 2008-2-1

No differential result

No differential result because the peak of WBC histogram lies in the middle of the histogram and thus cannot identify the type of peak cells
Version: 1.3, Update Date: 2008-2-1

Increased nucleated erythrocytes or interference or inadequate lyse

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

Version: 1.3, Update Date: 2008-2-1

Thank you!

Version: 1.3, Update Date: 2008-2-1