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Fruits: as all we know is the major content of daily food habitat. Fruits is defined as the flowering part of the plant, they are ripened part of the ovary of plant together with adjacent tissues. Fruits are fleshy or pulpy in character often juicy and usually sweet with fragment, aromatic flavors. According to the major fruits that we have taken, like apple, grapes, sapota and orange. As it is known those fruits contain different level of vitamins, proteins, carbohydrate and minerals. These contents are measured here by the methods of Lowry extraction techniques, phosphate buffer and Benedicts quantitative process. Those fruits are also tested for there moisture content and ash content.
CLASSIFICATION OF FRUITS
Fruits can be classified in to four types as follows:
SIMPLE FRUITS e.g. grapes COMPOUND FRUITS e.g. orange ACCESSORY FRUITS e.g. apple MULTIPLE FRUITS e.g. pineapple
ESTIMATION OF PROTEIN BY LOWRYS METHOD MATERIALS USED:Alkaline copper reagent: Folin phenol reagent: Samples (extracted protein)
PROCEDURE:
sample of fruits are taken in four test tubes
Then each of the samples was diluted with 1ml of folin phenol reagent. Then each of the samples was centrifuged and the supernatant were collected. 0.5ml of four different samples was taken in four different test tubes. Then 5ml of alkaline copper reagent added to each test tube and incubated at room temperature for 10minutes. To each test tube 0.5ml folin phenol reagent was added and contents were mixed well. Blue colour developed was read in calorimetrically at 640nm.
OD AT
OD VALUES
0.196
GRAPES
ORANGE
0.285
640nm
0.423
SAPOTA
0.421
OD
SAMPLES
AMOUNT(ml)
OD VALUES
APPLE
1.21
AT
GRAPES
1.36
ORANGE
2.54
540nm
SAPOTA
2.52
SL.NO
VOLUM E OF BQR
BURETTE READING
CONCORDANT VALUE
INDICATOR
INITIAL 1 1 5 5 0 0
SA M P L ES
GRAPES APPLE ORANGE SAPOTA 5 5 5 5 0 0 0 0 1.2 1.3 1.8 0.6 1.2 1.3 1.8 0.6 1.2 1.3 1.8 0.6 SELF SELF SELF SELF
RESULTS OF TITRATION
1GRAM OF GRAPES CONTAINS = 395.8Mg OF GLUCOSE 1 GRAM OF APPLE CONTAINS = 353.84Mg OF GLUCOSE 1 GRAM OF ORANGE CONTAINS = 255.55 Mg OF GLUCOSE 1 GRAM OF SAPOTA CONTAINS = 766.66 Mg OF GLUCOSE