Blood Groups

Ex 26

Blood Groups
Antigen agglutinogen Antibody agglutinin Reaction: agglutination
ABO Blood Type
A B AB O

Ag
A B AB -

Ab
Anti - B Anti - A Anti-A, anti-B

Anti - A
+ + -

Anti - B
+ + -

Rh Blood Type

Anti - D

Rh (+)

Agglutination

Rh (-)

No agglutination

 Material: Lancet 70% alc 2 slides/member (3members) Typing sera (anti-A, anti-B, anti-D)

** sharps container yellow bag

 1. 2. 3. 4. 5.

Procedure: Label slides A, B, D Sterilize finger 70% alc Puncture finger Wipe 1st drop of blood Place 1gtt of blood on slide A slide B slide D 6. Press punctured finger on cotton 7. Add 1gtt of anti A on slide A add 1gtt of anti B on slide B add 1gtt anti D on slide D 8. Mix blood and typing sera using applicator stick (1 per slide) 9. Gently tilt slide back and forth for 1min 10. Observe reaction (clumping) under fluorescent microscope 11. Record result