Documente Academic
Documente Profesional
Documente Cultură
Matthew L. Paden, MD Pediatric Critical Care Emory University Childrens Healthcare of Atlanta at Egleston
Primary unit of the kidney is the nephron 1 million nephrons per kidney Composed of a glomerulus and a tubule Kidneys receive 20% of cardiac output
Renal Lecture Required Picture #1
Aorta Renal artery interlobar arteries interlobular arteries afferent arterioles glomerulus efferent arterioles In the cortex peritubular capillaries In the juxtamedullary region vasa recta Back to the heart through the interlobular intralobar renal veins
Determined by the hydrostatic and oncotic pressure within the nephron Hydrostatic pressure in the glomerulus is higher than in the tubule, so you get a net outflow of filtrate into the tubule Oncotic pressure in the glomerulus is the result of non-filterable proteins
capillary endothelium is surrounded by a basement membrane and podocytes Foot processes of the podocytes form filtration slits that :
Allow for ultrafiltrate passage Limit filtration of large negatively charged particles
Less than 5,000 daltons = freely filtered Large particles (albumin 69,000 daltons) not filtered
Tubular Function
Proximal
Most of reabsorption occurs here Fluid is isotonic with plasma 66-70% of sodium presented is reabsorbed Glucose and amino acids are completely reabsorbed
Tubule Function
Loop
of Henle
Urine concentration and dilution via changes in oncotic pressure in the vasa recta Descending tubule permeable to water, impermeable to sodium Ascending tubule actively reabsorbs sodium, impermeable to water
Tubular Function
Medullary
thick ascending limb critical for urinary dilution and most often damaged in ARF
Lasix use here inhibits the Na-K-2Cl ATPase which in the face of ARF, may decrease oxygen consumption and ameliorate the severity of the ARF
Tubular Function
All
Tubule Function
Distal
Tubule
Re-absorption of another ~12% of NaCl Proximal segment impermeable to water Distal segment is the cortical collecting duct and secretes K and HCO3
Tubular Function
Collecting
Duct
Aldosterone acts here to increase Na reuptake and K wasting ADH enhances water re-absorption Urea re-absorption to maintain the medullary interstitial concentration gradient
failure is defined as the cessation of kidney function with or without changes in urine volume Anuria UOP < 0.5 cc/kg/hour Oliguria UOP more than 1 cc/kg/hour
Less than?
Non-oliguric , 30% Oliguric Non-oliguric associated with better prognosis and outcome Overall, the critical issue is maintenance of adequate urine output and prevention of further renal injury.
Renal
<20
FENa
Renal Failure Index UNa Specific Gravity Uosm Uosm/Posm
<1%
<1% <20 mEq/L >1.020 >1.3
>2%
>1% >40 mEq/L <1.010 <1.3
Ultrasound
Structural anomalies polycystic, obstruction, etc. ATN
poor corticomedullary differentiation Increased Doppler resistive index (Systolic Peak Diastolic peak) / systolic peak
Insult
to tubular epithelium causes release of vasoactive agents which cause the constriction
Doubling of RBF in first 2 weeks of life Triples by 1 year Approaches adult levels by preschool
Renal
Renin-Angiotensin Axis
For the one millionth time. Hypovolemia leads to decreased afferent arteriolar pressure which leads to decreased NaCl re-absorption which leads to decreased Cl presentation to the macula densa which increases the amount of renin secreted from the JGA which increases conversion angiotensinogen to AGI to AGII which increases Aldosterone secretion from the adrenal cortex and ADH which leads to increased sodium and thus water re-absorption from the tubule which increases your blood pressurewhew
Hyperplasia of JGA with increased renin granules seen in patients and experimental models of ARF Increased plasma renin activity in ARF patients Changing intra-renal renin content modifies degree of damage
Feed animals high salt diet (suppress renin production) renal injury less renal injury than those fed a low sodium diet
You can also ameliorate renal injury by induction of solute diuresis with mannitol or loop diuretics (neither affect the RAS) No change in renal injury in animals given ACE inhibitors, competitive antagonist to angiotensin II
Overall,
Prostaglandins
PGE
2 and PGI
Very important for renal vasodilation, especially in the injured kidney Act as a buffer against uncontrolled A2 mediated constriction
If you constrict the afferent arteriole, you will decrease GFR
The
Adenosine
Potent
renal vasoconstrictor
Peripheral vasodilator
Infusion
of methylxanthines (adenosine receptor blockers) inhibits the decrease in GFR that is seen with tubular damage Some animal models show that infusion of methylxanthines lessen renal injury in ARF
Adenosine
But.
Methylxanthines have lots of other actions besides adenosine blockade Adenosine is rapidly degraded after production Intra-renal adenosine levels diminish very rapidly after reperfusion, but the vasocontriction remains for a longer period Finally, if you block ADA, creating higher tissue adenosine levels, and then create ischemia you actually get an enhancement of renal recovery
Endothelin
21 amino acid peptide that is one of the most potent vasoconstrictors in the body
Its role in unclear in normal state In ARF, overproduction by cells (both in and outside of the kidney) leads to decreased afferent flow and thus decreased RBF and GFR
Stimulates ANP release at low doses and can increase UOP Anti-endothelin antibodies or endothelin receptor antagonists decrease ARF in experimental models
Nitric Oxide
Produced
by multiple iso-enzymes of NOS In addition to its role in vasodilation, likely has a role in sodium re-absorption
Important
RBF Exact mechanisms not worked out completelyat least when Rogers was written.
Nitric Oxide
Confusing
results
Ischemic rat kidney model inducing NOS causes increasing injury Hypoxic tubular cell culture model inducing NOS causes increasing injury But if you block NOS production, you get worsening of renal function and severe vasoconstriction
Nitric Oxide
So
stimulation of NO in the renal vasculature will modulate vasoconstriction and lead to lesser injurybut That same induction of NO in the tubular cells will cause increased cytotoxic effects
Dopamine
Dopamine
arteriole Dilation of renal vasculature at low doses, constriction at higher doses Also causes naturesis (? Reason for increased UOP after starting) Renal dose dopamine controversy.
Renal vasoconstriction is a well documented cause of ARF Renal vasodilation does not consistently reduce ARF once established
Although renal hemodynamic factors play a large role in initiating ARF, they are not the dominant determinants of cell damage
ARF - Pathophysiology
Damage
is caused mostly by renal perfusion problems and tubular dysfunction Usual causes
ARF Pathophysiology
Hypo-perfusion
Well perfused kidney 90% of blood to cortex Ischemia increased blood flow to medulla Outcome may be able to be influenced by restoration of energy/supply demands
Lasix example
ARF - Pathophysiology
Oxidative
damage
ARF - Pathophysiology
Ischemia
Damage to mitochondrial membrane and change of xanthine dehydrogenase (NAD carrier) to xanthine oxidase (produces O2 radicals) Profound utilization of ATP 5-10 minutes of ischemia you use ~90% of your ATP
Make lots of adenosine, inosine, hypoxanthine
Adenylosuccinate
Adenosine
IMP
Hypoxanthine
H20 O2 Xanthine H2O2
Inosine
H20 O2
Uric Acid H20 O2
H2O2
CO2
Allantoin
ARF - Pathophysiology
Once you get reperfusion, the hypoxanthine gets metabolized to xanthine and uric acid each creating one H2O2 and one super-oxide radical intermediate Reactive oxygen species oxidize cellular proteins resulting in:
Change in function/inactivation/activation Loss of structural integrity Lipid peroxidation (leads to more radical formation) Direct DNA damage
ARF Pathophysiology
Amount
ARF Pathophysiology
Amount
This
ARF - Prevention
Maintenance
of blood flow
Avoidance
of toxins
Easy
on paper.difficult in practice
ARF - Prevention
Lasix
Mannitol
ARF - Prevention
Renal
No human trials More rapid improvement of renal function in animals Increased uptake of ADP to form ATP or cell membrane stabilization as a possible cause
Thyroxine
ARF - Prevention
ANP
Theophyline
Growth Factors
Mechanism of toxicity
Disassociation to ferrihemate, a tubular toxin, in acidic urine Tubular obstruction Inhibition of glomerular flow by PGE inhibition or increased renin activation
Treatments (?)
Aggressive hydration to increase UOP Alkalinization of urine Mannitol/Furosemide to increase UOP ?Early Hemofiltration
Acid Nephropathy
ARF - Management
Electrolyte
management
Sodium
Hyponatremia fluid restriction first, 3% NaCl if AMS or seizing
Potassium
Calcium/Bicarb/Glucose/Insulin/Kayexalate Hemodialysis
ARF - Management
Nutrition
management
Peritoneal dialysis
Advantages
Disadvantages
Simple to set up & perform Easy to use in infants Hemodynamic stability No anti-coagulation Bedside peritoneal access Treat severe hypothermia or hyperthermia
Unreliable ultrafiltration Slow fluid & solute removal Drainage failure & leakage Catheter obstruction Respiratory compromise Hyperglycemia Peritonitis Not good for hyperammonemia or intoxication with dialyzable poisons
Intermittent Hemodialysis
Advantages
Disadvantages
Maximum solute clearance of 3 modalities Best therapy for severe hyperkalemia Limited anti-coagulation time Bedside vascular access can be used
Hemodynamic instability Hypoxemia Rapid fluid and electrolyte shifts Complex equipment Specialized personnel Difficult in small infants
Continuous Hemofiltration
Advantages
Disadvantages
Easy to use in PICU Rapid electrolyte correction Excellent solute clearances Rapid acid/base correction Controllable fluid balance Tolerated by unstable pts. Early use of TPN Bedside vascular access routine
Systemic anticoagulation (except citrate) Frequent filter clotting Vascular access in infants
Oliguria/Anuria Hyperammonemia Hyperkalemia Severe acidemia Severe azotemia Pulmonary Edema Uremic complications Severe electrolyte abnormalities Drug overdose with a filterable toxin Anasarca Rhabdomyolysis