Sunteți pe pagina 1din 29

BLOOD

COLLECTION AND
PROCESSING


M KASTHURI BAI
OBJECTIVE
After following this lecture, students must
be able to understand:

Processes involved in blood donation
center.
Important criteria in donor selections.
The donor unit processing.
LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history
Physical examination
Phlebotomy
Adverse donor reaction
Donor unit processing
Storage of donated blood
Donation process
Donor area - attractive
- open at convenient hours
- comfortably ventilated
- understanding
- professional
- well trained personnel
LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history
Physical examination
Phlebotomy
Adverse donor reaction
Donor unit processing
Storage of donated blood
Registration
Information fully identify the donor
Details
Date & time donation
Name and address
Contact no.
Gender
Age/ date of birth
Previous transfusion/ TTD
LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history
Physical examination
Phlebotomy
Adverse donor reaction
Donor unit processing
Storage of donated blood
Donor selection
Important in protecting safety of blood
supply
To obtain truthful & accurate medical
history to ensure safe transfusion
Protect donor confidentiality
Uniform donor history questionaire
LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history
Physical examination
Phlebotomy
Adverse donor reaction
Donor unit processing
Storage of donated blood
Medical history
To identify potential risk factor that could
jeopardize the health of donor or
compromise the safety of blood for
recipient
Donor deferred should be given full
explanation
Inform when they can return
Confidential unit exclusion
Opportunity provided for donor to indicate
blood collected should not be used for
transfusion
LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history
Physical examination
Phlebotomy
Adverse donor reaction
Donor unit processing
Storage of donated blood
Physical examination
Donor should be in good health

Donor deferred if under influence of
alcohol or drugs or with obvious signs of
cold or other illness
Physical examination
Determination of Hb and hct levels
Hb : 12.5 16.0 g/dl (female)
: 13.5 18.0 g/dl (male)
Blood pressure
Systolic : < 180 mmHg
: < 100 mmHg
Pulse
Pulse rate should be between 50 100
beats per minute
Physical examination
Temperature
Orally should not exceed 37.5C (99.5 F)
Weight
50 kg (110lb ) or more 450 mL of blood
< 50 kg not more than 12% of blood volume
Formula : Body wt(lbs) x 450 = allowable amt
110 (lbs)
Skin lesion free at venipuncture
LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history
Physical examination
Phlebotomy
Adverse donor reaction
Donor unit processing
Storage of donated blood
Phlebotomy
Bag selection
Identification
Selection of vein
Phlebotomy & collection of samples
Care of donor
Bag selection
FDA approved

Pyrogen free, sterile, sufficient
anticoagulant

According to need and amount of
collection
Single / triple
Identification
Assigning identification number to each
individual donation
Allows donation process to be traced
and tracked
Numeric or alphanumeric no should be
placed on donation record, primary bag,
all satelite bags and sample tubes

Selection of vein
Both arm inspected for lesions that may
indicate IV drug use or signs of infection
Blood drawn from large, firm vein in the
antecubital area of the arm
Tourniquet or BP set inflated to 40
mmHg
Aseptic technique must be used
Phlebotomy and collection of
samples
Final check to verify donors
identification
During collection blood mixed with
anticoagulant
Amount collected monitored carefully so
total does not exceed 525 mL
PPE
Care of donor
After removing needle from vein
Apply firm pressure with sterile gauze
Donor recline on bed of donor chair for few
minutes
Allow to sit. Follow donor to refreshment
area
Post phlebotomy care
Eat and drink before leaving
Drink more fluid in next 4 hours
Avoid alcohol until you have eaten
Care of donor
Do not smoke for 30 minutes
If bleeding persists, raise hand and apply pressure
Fainting/ dizziness occur lie down with head btw
knees
If symptoms persists contact BDC
Resume all normal activity if asymptomatic
Remove bandage within few hours
Maintain high fluid intake for approx 3 days


THANK DONOR & ENCOURAGE REPEAT DONATION
LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history
Physical examination
Phlebotomy
Adverse donor reaction
Donor unit processing
Storage of donated blood
Adverse donor reaction
Personnel should be trained in CPR
Syncope (fainting)
Caused by sight of blood
Symptom: weakness, sweating, dizziness,
pallor. Unconcious
Deep breathing and hyperventilation
Fainting
Place donor on back with feet raised above head
Loose tight clothing
Adequate airways
Aromatic spirits

LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history
Physical examination
Phlebotomy
Adverse donor reaction
Donor unit processing
Storage of donated blood
Donor unit processing
ABO & Rh grouping
Antibody screening
Screening of transfusion transmitted
diseases
Syphilis
Hbs Ag
Anti HCV
Anti HIV
Anti HTLV I / II
CMV
LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history
Physical examination
Phlebotomy
Adverse donor reaction
Donor unit processing
Storage of donated blood
Storage of donated blood
Blood unit Storage condition Storage duration
Red blood cell 1 4 C Max 42 days
Platelets RT 5 days on agitator
Fresh frozen plasma frozen 1 year
Cryoprecipitate frozen 1 year
Granulocyte RT Transfused within 24
hours
Thank you

S-ar putea să vă placă și