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ب اْلَعاَلِمي َ
ن ل َر ّ
ي َومََماِتي ِّ
حَيا َ
سِكي َوَم ْ
لِتي َوُن ُ
ص َ
ن َ
ل ِإ ّ
ُق ْ
سِلِمي َ
ن ل اْلمُ ْ
ت َوَأَنا َأّو ُ
ك ُأِمْر ُ
َٰ
ك َلُهۖ َوِب َٰذِل
شِري َ
ل َ
َ
صدق ال العظيم
سورة النعام ١٦٣ - ١٦٢
-This series of presentations will provide a 'step by
step' guide for Molecular Microbiology Laboratory
Techniques.
- According to your experiment, you will need specific lab Equipments which will
be mentioned later on.
-Take a tour with the person in charge or a senior lab member: to Identify your
new lab.
Keep small notebook : for miscellaneous information such as:
* Location of cold rooms, fridges, freezers, shelves, containers (labels and
contents of each).
* Location of different reagents in the lab such as acids, highly flammable
substances - amount allowed to keep on your bench.
* Location of disposal containers for different stuff such as needles, broken
glass.
* Location of the glass-ware washing facility, autoclave – number and time of
different cycles/day.
* Names of support stuff for good communication.
* Different hoods – their uses.
* Other common use facilities and machines such as centrifuges – their
differences.
* Emergency equipments such as eye wash.
Very Important
For :
- Such a program can allow you to make a rapid search for certain information,
and also considered a backup for your techniques for future use.
*Induction Course
- Attendance is very important !!
- Gives you information about:
- Safety measures:
- 'COSHH' regulations
'Control of Substances Hazardous to Health‘
- Critical personal safety such as ‘eye safety’ when using a UV illuminator.
Read well =
Good Decision
- Next day work to avoid loosing time for just preparing solutions,
sterilization of instruments, or ordering stuff (already finished).
Much easier
- Use a wide-tip marker
- Write details of the solution on the bottle or the flask
- Molecular work samples are usually contained in:
Eppendorf tube - Cryovial - bijoux - Universal tube - Falcon tube.
- On the side of the eppendorf, write: date, and a clear hint about its contents.
- Specify a notebook for registering your samples' data, including the following:
- Sample Number [written on the cap, and side].
- Date.
- Container [for easy identification as eppendorf, bijoux, universal,...].
- Where to find it 'location' [e.g: fridge, -20 freezer, or -80 freezer,
liquid nitrogen, or cold room (4°C)].
- Contents in detail.
-Send solutions requiring sterilization to the
autoclave.
[Do NOT forget to stick 'steam indicator tape'
on the bottles or flasks to ensures efficient
sterilization],
OR
- Use filtration according to the solution contents
(e.g: if it contains sugars which are affected by
high temperature).
*Product name.
*Product catalogue number
*Company name
*Price
*Delivery fees
Thank You for Attention