Sunteți pe pagina 1din 26

‫بسم ال الرحمن الرحيم‬

‫ب اْلَعاَلِمي َ‬
‫ن‬ ‫ل َر ّ‬
‫ي َومََماِتي ِّ‬
‫حَيا َ‬
‫سِكي َوَم ْ‬
‫لِتي َوُن ُ‬
‫ص َ‬
‫ن َ‬
‫ل ِإ ّ‬
‫ُق ْ‬
‫سِلِمي َ‬
‫ن‬ ‫ل اْلمُ ْ‬
‫ت َوَأَنا َأّو ُ‬
‫ك ُأِمْر ُ‬
‫َٰ‬
‫ك َلُهۖ َوِب ‌َٰذِل‬
‫شِري َ‬
‫ل َ‬
‫َ‬
‫صدق ال العظيم‬
‫سورة النعام ‪١٦٣ - ١٦٢‬‬
-This series of presentations will provide a 'step by
step' guide for Molecular Microbiology Laboratory
Techniques.

-This course targets mainly those who are


enthusiastic to contribute to science development.

-So, if you have a limited knowledge in that field,


hopefully by following these presentations you will be
able to do your 'first' molecular experiments.
Introduction
To Molecular
Microbiology
Lab
Usually better !
*Essentials
- Lab coat.
- Disposable gloves [suitable size 'S, M, L', latex or nitrile].
- Timer.
- Calculator.
- Set of different 'size range' pipettes (0.5-10ul, 10-200ul, 100-1000ul), plastic
Pasteur pipettes.
- Permanent markers, wide and fine tips.
- Stationary tools [pens, pencils, eraser, scissors, glue stick, highlighters

'different colours'] for clear presentation of data in the lab book.

- According to your experiment, you will need specific lab Equipments which will
be mentioned later on.
-Take a tour with the person in charge or a senior lab member: to Identify your
new lab.
Keep small notebook : for miscellaneous information such as:
* Location of cold rooms, fridges, freezers, shelves, containers (labels and
contents of each).
* Location of different reagents in the lab such as acids, highly flammable
substances - amount allowed to keep on your bench.
* Location of disposal containers for different stuff such as needles, broken
glass.
* Location of the glass-ware washing facility, autoclave – number and time of
different cycles/day.
* Names of support stuff for good communication.
* Different hoods – their uses.
* Other common use facilities and machines such as centrifuges – their
differences.
* Emergency equipments such as eye wash.
Very Important
For :

- Registering every day experimental steps in detail 'ASAP'.

- Loose leaf, A4 papers.


-Ring binder: is the most suitable as it allows you to add any notes or lecture
handout in the middle.
-Each and every small notice must be included: may appear of no value at that
time, but soon you will discover its importance in interpretation of
experiment.
-Take along the relevant textbooks which contain details about your
experiment.
- Software such as 'Data base program’
can be used to keep the results.

- However, using such a programme Does NOT decrease the importance of


having a lab book which must be with you in the lab while doing your
experiments [outside the working space of the bench] till you are able to
access a computer to put your data on.

- Such a program can allow you to make a rapid search for certain information,
and also considered a backup for your techniques for future use.
*Induction Course
- Attendance is very important !!
- Gives you information about:

- Safety measures:
- 'COSHH' regulations
'Control of Substances Hazardous to Health‘
- Critical personal safety such as ‘eye safety’ when using a UV illuminator.

- Regulations of the department:


- Work hours – different authorities for any queries e.g, safety.
- Permission for Late hours work.
- Restricted work areas, advance permission.
- Lab Machines which require special training prior to individual use
(e.g: Ultra-centrifuges).
Good proposal
1) Reading Literature As Much As You Can.
- Takes time, but is essential before selecting a topic.

Design of your experiment - other fine tunings.


2) Accurate 'Time plan' Regularly check if you
get an “acceptable part” of the expected results –
proportional to time

3) Be ready to shift your experiment to a


modified way in case of facing difficulty before loosing
more time, but Avoid Major Change “ = New Plan”.
4) Think of availability of 'all' requirements of a good research.

- Who will provide you if not commercially available.

- It is better to catch them in your hand before starting the


project [e.g.; control samples, antibodies for immunological assays
'Western blots, ELIZA' of new experimental product such as
recombinant proteins].
of kits you intend to buy for future steps.

Read well =
Good Decision
- Next day work to avoid loosing time for just preparing solutions,
sterilization of instruments, or ordering stuff (already finished).

- Advance booking of equipments [such as hoods, ultra-centrifuges,


freeze-dryer] which are frequently used by most of the lab members.
- Register your booking details in the log book of each equipment.

- Moving to a next step in your experiment: Do Not Do Except after


being sure of the validity of the “previous” step as long as you have the tools to
check.
It’s ‘Critical’ !!!
details

Much easier
- Use a wide-tip marker
- Write details of the solution on the bottle or the flask
- Molecular work samples are usually contained in:
Eppendorf tube - Cryovial - bijoux - Universal tube - Falcon tube.

The Most difficult (small surface area available for writing).


*Follow a consistent and clear way for labelling all samples:

- Use fine-tip marker - write a sample number on the eppendorf cap:


(Easily select the tubes you need by numbers while in the box).

- On the side of the eppendorf, write: date, and a clear hint about its contents.

- Specify a notebook for registering your samples' data, including the following:
- Sample Number [written on the cap, and side].
- Date.
- Container [for easy identification as eppendorf, bijoux, universal,...].
- Where to find it 'location' [e.g: fridge, -20 freezer, or -80 freezer,
liquid nitrogen, or cold room (4°C)].
- Contents in detail.
-Send solutions requiring sterilization to the
autoclave.
[Do NOT forget to stick 'steam indicator tape'
on the bottles or flasks to ensures efficient
sterilization],
OR
- Use filtration according to the solution contents
(e.g: if it contains sugars which are affected by
high temperature).

- Write your name, lab number, solution name,


date, storage temperature, and shelf life on your
containers before sending to the autoclave.
- Must be ready to put any order As Soon As
You Can.

- Keep details of each product for future use:

*Product name.
*Product catalogue number
*Company name
*Price
*Delivery fees
Thank You for Attention