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Lab 4 - Enzymes and The Catalase Lab #NSB2014 - students will be able to: Determine the household use of Hydrogen Peroxide Recognize different enzymes and their characteristics Compare and contrast catalase in animal vs. Plant cells Explain why temperature and pH would have an effect on enzyme activity.
Lab 4 - Enzymes and The Catalase Lab #NSB2014 - students will be able to: Determine the household use of Hydrogen Peroxide Recognize different enzymes and their characteristics Compare and contrast catalase in animal vs. Plant cells Explain why temperature and pH would have an effect on enzyme activity.
Lab 4 - Enzymes and The Catalase Lab #NSB2014 - students will be able to: Determine the household use of Hydrogen Peroxide Recognize different enzymes and their characteristics Compare and contrast catalase in animal vs. Plant cells Explain why temperature and pH would have an effect on enzyme activity.
#NSB2014 1 After completing this activity, students will be able to: Determine the household use of Hydrogen Peroxide Recognize different enzymes and their characteristics Compare and contrast the catalase in animal vs. plant cells Explain why temperature and pH would have an effect on enzyme activity
#NSB2014 2 What are Enzymes? Enzymes are Biological Catalysts - substances that increase the speed of chemical reactions. When an enzyme is present it takes less energy to start a chemical reaction (activation energy) Enzymes help maintain homeostasis Enzymes facilitate metabolism which is essential for life
#NSB2014 3 Enzymes are proteins. Proteins are made up of amino acids Enzymes work with a very specific substrate (the molecule acted on by an enzyme) to speed up a reaction
#NSB2014 4 Enzymes have one or more deep folds on its surface These folds form pockets called active sites Enzymes act on the substrate, by fitting into the active site like a puzzle #NSB2014 5 Enzymes and their substrates Each enzyme has a definite 3-D shape that allows it to bind with its substrate Each enzymes acts only on one specific substrate, which means they break down specific substances (like a lock and key)
#NSB2014 6 Why do we need enzymes? Enzymes work to: Turn leaves colors in the fall (think of the yellows, oranges, reds, purples) Ripen foods (this is what makes your bananas brown, or apples get mushy), Digest foods after they are eaten, Produce DNA and hormones Without enzymes, life as we know it is not possible #NSB2014 7 What affects enzymes in a reaction?
Temperature pH
#NSB2014 8 Temperature There is one temperature at which specific enzymes work best. This optimal temperature is usually around human body temperature (37.5 o C) for the enzymes in human cells. Above this temperature the enzyme structure begins to break down (denature) (ie. fever)
#NSB2014 9 pH Enzymes have an optimal pH. However the optimum is not the same for each enzyme. Enzymes are found at different places in your body, and in the environment pH would be different in the body vs. stomach
#NSB2014 10 Factors that affect enzyme activity Temperature Low temperature (cold) = reactions occur slowly High temperature (hot) = enzymes denature, which can change the structure of proteins and alter the enzymes function forever pH pH scale goes from 0-14 pH of pure water is 7 Basic (pH higher than 7); Acidic (pH lower that 7) Changes in pH can also change the structure of proteins and alter the enzymes function. Organisms can only tolerate (stand) small changes in pH because every cell has a particular pH at which it functions best (homeostasis).
#NSB2014 11 Examples of Enzymes and their Substrates Enzyme Substrate Amylase---------------------Starch Maltase----------------------Maltose Sucrase----------------------Sucrose Lipase------------------------Lipids (Fats) Pepsin------------------------Proteins Catalase--------------Hydrogen Peroxide
#NSB2014 13 Catalase 2H 2 0 2 2H 2 0 + 0 2 (gas) The products of the above reaction are oxygen gas and water, two non-poisonous molecules. In living cells, the oxygen can be used for cellular respiration and the water can be excreted.
#NSB2014 14 H 2 O 2 is similar to H 2 O Hydrogen peroxide is chemically very similar to water. Water = H 2 O, Hydrogen peroxide = H 2 O 2 . In chemistry, things can change a lot when you change the formula.
#NSB2014 15 An extra atom of oxygen also makes hydrogen peroxide a very different chemical, which can be dangerous if it is concentrated. That is why the H 2 O 2 you get at the grocery is only 3%. The rest is plain water and it is diluted enough to make it safe for household use.
#NSB2014 16 Scientific Terminology (used in this lab) Control the constant between experimental groups Independent variable the one variable that changes between experimental groups Dependent variable measured variable #NSB2014 17 POST LAB What causes the bubbles? Why did some substances bubble more than others? #NSB2014 18