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Il existe un
vieux débat en
neurosciences
sur les
contributions
relatives du
thalamus et du
cortex dans le
processus qui
produit
l’activité
cérébrale
présente dans
le cortex
sensoriel
primaire
Les deux positions dans ce débat
Le monde Le système
extérieur cognitif crée son
comporte des propre monde, et
règles fixes; il toute son
précède l’image apparente
qu’il projette sur solidité repose
le système sur les lois
cognitif dont la internes de
tâche est de l’organisme
saisir – le monde
– de manière
appropriée
A Lejeune (que 4
Somatosensory Corticothalamic
Projections: Distinguishing
Drivers from Modulators
par
Drivers et Modulators
to cortex from the
periphery
=DRIVER
L’aire corticale 1
étant une aire
sensorielle primaire, sa
couche 4
est la cible de
issues de sa couche 6
Les inputs
cholinergiques Drivers et Modulators (
du niveau 5
pulvinar.
Il ne reçoit aucune
information
DRIVER
sensorielle,
mais il est la cible
de
connexions projetées
(A) (B)
par
les neurones des
couches 4 et 5 de
l’aire corticale 1.
Il est aussi connecté
avec l’aire 2,
projetant sur sa
niveau 5 est
Drivers et Modulators (
limitée àla
morphologie
(Flowery) – Li et al.
(2003)
Le
but de cette
recherche est
d ’ approfondir cette
hypothèse de Li et al .
( 2003 ) qui distinguent –
sur des bases
morphologiques – deux
groupes d ’ inputs sur le
Lateral Posterior
Thalamus ( LPT ):
- Un groupe avec
‘ synaptic depression ’
inhibiteur ;
- - un groupe avec
‘ synaptic
facilitation ’
stimulation … sans
toutefois identifier
On la va donc
source activer les axones des couches 5 et 6 sur une
corticale
couche( niveau 5 OU 6? )
thalamocorticale et enregistrer les réponses sur le
noyau ventral postérieur ( 1er ordre FO ) et le noyau médial
postérieur ( HO )
First-order relays represent the first transmission
Design de lato cortex of particular type of information from the periphe
recherche Higher-order relays serve to transmit information between
cortical areas via a cortico-thalamo-cortical route
comparaison avec
1st O HO
facilitation, and the EPSPs showed both NMDA and AMPA receptor component plus an
mGluR1 component
A Lejeune 10
methods
A Lejeune 11
FIG. 1. Mouse
thalamocortical
slice preparation
as
seen in recording
chamber
A: lower-power view of
slice. Barrel cortex (BC)
A Lejeune 13
Rappel: D e s a n ta g o n iste s so n t u tilisé s su r le s
A receptor ré ce p te u rs G A B A ( a e t b ),
antagonist is… A M PA , m G lu R , m G lu R 5
A receptor 1] In pharmacology
antagonist is a ,antagonists have affinity
type of receptor but no efficacy for their
ligand or cognate receptors, and
drug that does binding will disrupt the
not provoke a interaction and inhibit the
biological
function of an agonist or
inverse agonist at receptors.
response itself Antagonists mediate their
upon binding to effects by binding to the
a receptor, but active site or to allosteric
blocks or sites on receptors, or they
dampens agonist may interact at unique
-mediated binding sites not normally
responses.[ A Lejeune
involved in the biological 14
Rappel:
A receptor
antagonist is…
(2)
. Antagonist activity
may be reversible or
irreversible
depending on the
longevity of the
antagonist–receptor
complex, which, in
turn, depends on the
nature of antagonist
receptor binding.
The majority of drug
antagonists achieve
their potency by
competing with
endogenous ligands
or substrates at
structurally-definedA Lejeune 15
Paired-pulse facilitation:
definition
When a presynaptic neuron receives
two stimuli in rapid succession, the
postsynaptic response will commonly
be larger for the second than for the
first pulse — a phenomenon known as
paired-pulse facilitation (PPF). Now
here's an easy question for every
neurophysiologist: what is the
mechanism responsible for PPF?
Although most people will quickly
point in the direction of 'residual
A Lejeune 16
Paired-pulse depression
A Lejeune 18
FIG. 1. Mouse
thalamocortical
slice preparation
as
seen in recording
chamber
A: lower-power view of
slice. Barrel cortex (BC)
A Lejeune 24
of the
corticothalamic C o rtico th a la m ic a xo n s fro m la ye rs 5
pathway a n d 6 a re o rth o g ra d e ly la b e le d b y
b io cytin io n to p h o re sis in to th e b a rre l
co rtex
A: injection site
(arrow) in BC.
Also visible is the
IC. The lettered
boxes refer to the
higher power views
in B–D.
B: labeled axons
running between
external and
internal capsules.
C: terminal
boutons of
cortical fibers in
thalamic reticular A Lejeune 25
corticothalamic C o rtico th a la m ic a xo n s fro m la ye rs 5
pathway
a n d 6 a re o rth o g ra d e ly la b e le d b y
b io cytin io n to p h o re sis in to th e b a rre l
co rtex
Many axons ramify in
the thalamic reticular
and ventral posterior
nuclei and terminate
there, but a few
continue further to the
posterior medial
nucleus.
D: terminal boutons
of cortical axons in
the ventral posterior
medial nucleus and
POm.
Several of the larger
boutons in the
posterior medial
A Lejeune 27
subcortical A : e x a m p le o f e v o k e d E P S P s.
stimulation in a cell B : p a ire d - p u lse fa cilita tio n a n d g ra d e d
in the ventral re sp o n se o f E P S P s
posterior medial C : e v id e n ce o f a n m G lu R 1 co m p o n e n t.
nucleus D : co n tro l. LY 3 6 7 3 8 5 ( 5 0 M ) a p p lie d to th e
ce ll d o e s n o t sh o w a n y e ffe ct o n its m e m b ra n e
The EPSPs evoked p o te n tia l
by low frequency
stimulation (LFS 400 A,
application of MK-801
and DNQX
(top). With these
antagonists present,
high-frequency
stimulation (HFS, 125
(50 M, bottom).
The line below the
A Lejeune 29
EPSPs evoked from
stimulation of layer A : e xa m p le o f e vo ke d E P S Ps
6 of barrel cortex in B : p a ire d - p u lse fa cilita tio n a n d g ra d e d
a cell of the
posterior medial re sp o n se o f E P S Ps
nucleus C : e vid e n ce o f a n m G lu R 1 co m p o n e n t
The EPSPs
evoked by LFS (13 Hz
of MK-801 and
DNQX (top). With these
antagonists present,
HFS (125 Hz for 600 ms)
evoked a sustained
EPSP (2nd trace) that is
an mGluR1 antagonist
(50 M, bottom).
A Lejeune 30
stimulation of layer A : e x a m p le o f e v o k e d E P S P s
5 of barrel cortex in B : p a ire d - p u lse d e p re ssio n
a cell of the C : a ll- o r- n o n e re sp o n se o f E P S P s is a p p a re n t
posterior medial fro m re sp o n se s to in cre a sin g stim u la tio n
nucleus in te n sitie s
D : la ck o f m G lu R co m p o n e n t
After
blocking
EPSPs with
DNQX
and MK-801,
neither LFS
(50 Hz for
855 ms, top)
nor HFS
(125 Hz for
855 ms,
bottom)
A Lejeune 31
of graded and all-
or-none EPSPs
from cells of the
posterior medial
nucleus
The 3
examples of
all-or-none
responses (—
and ) reflect
stimulation
from layer 5,
whereas the 5
examples of
graded
responses
(- - - and )
A Lejeune 32
EPSPs evoked from
separate stimulation of
layers 5 and 6 of barrel
cortex in the same cell
of the posterior medial
nucleus
A, i and ii: paired-
pulse effects, showing
depression for layer 5
stimulation (Ai) and
facilitation for layer 6
stimulation (Aii).
B, i and ii:
recruitment
properties, showing all-
or-none response for
layer 5 stimulation (Bi)
and graded responses
for layer 6 stimulation
A Lejeune 33
EPSPs evoked from
separate stimulation of
layers 5 and 6 of barrel
cortex in the same
cellof the posterior
medial nucleus
C, i and ii:
contribution of
mGluRs. Application of
DNQX and MK-801
blocks EPSPs to LFS (9
Hz for 755 ms) both
from layer 5 (Ci, top)
and
from layer 6 (Cii, top).
A Lejeune 35
cortico-thalamo-
cortical
communication
involving
higher-order
thalamic relays.
This hypothesis suggests
that information arrives
initially at the cortical
level after transmission
through a 1rst-order (FO)
thalamic relay such as the
lateral geniculate nucleus,
ventral division of the
medial geniculate nucleus
(MGNv), or medial or
lateral VP. Further
corticocortical
communication in addition
to or perhaps instead of
direct pathways
involves transmission via
higher-order (HO)
thalamic relays, such as
the pulvinar (Pul),
magnocellular division of
the medial geniculate
nucleus or the POm. A
remaining issue is the
identity of direct
corticocortical pathways A Lejeune 36
Discussion
T h is h y p o th e sis su g g e sts th a t in fo rm a tio n
a rriv e s in itia lly a t th e co rtica l le v e l a fte r
tra n sm issio n th ro u g h a 1 rst- o rd e r ( F O )
th a la m ic re la y su ch a s th e la te ra l g e n icu la te
n u cle u s , v e n tra l d iv isio n o f th e m e d ia l
g e n icu la te n u cle u s ( M G N v ) , o r m e d ia l o r
la te ra l V P .
F u rth e r co rtico co rtica l co m m u n ica tio n in
a d d itio n to o r p e rh a p s in ste a d o f d ire ct
p a th w a y s in v o lv e s tra n sm issio n v ia h ig h e r-
o rd e r ( H O ) th a la m ic re la y s , su ch a s th e
p u lv in a r ( P u l) , m a g n o ce llu la r d iv isio n o f th e
m e d ia l g e n icu la te n u cle u s o r th e P O m .
A re m a in in g issu e is th e id e n tity o f d ire ct
co rtico co rtica l p a th w a y s a s d riv e r o r
A Lejeune 37
Une image
vaut mille
mots…
Review
Thalamic Relay Functions
and Their Role in
Corticocortical
Communication:
Generalizations from the
Visual System
R.W. Guillery1 and
S.Murray Sherman2, ,
1Department of Anatomy,
University of Wisconsin
School of Medicine, 1300
University Avenue,
Madison, WI 53706 USA
2
Department of
Neurobiology, State
University of New York,
Stony Brook, NY 11794
USA
Available online 22
January 2002.
A Lejeune 38
Questions
??
Somatosensory
Corticothalamic
Projections:
Distinguishing
Drivers from
Modulators
par
Iva Reichova et
S. Murray
Sherman
Journal of
Neurophysiolog
y, 2004
A Lejeune 39