DEPARTMENT OF

BIOCHEMISTRY
Introduction by
Miss Sumaira khan
Senior lecturer

1
 FACULTY
• Professor Dr Sajid Raza
MBBS,M-Phil
(Head of the Department biochemistry)

• Dr Sadia Haroon
MBBS,M-Phil
(Associate Prof)

Dr Waseem kausar
MBBS,M-Phil
(Assistant Prof)

• Ms Sumaira Sarwar Khan

M.Sc, M-Phil
(Senior lecturer)

2
Demonstrators

• Miss Maimoona (Msc Mphil Biochem)

• Dr Zeeshan (MBBS)
• Dr Mutassim (MBBS)
• Dr Sajjad (MBBS)
• Dr Adnan (MBBS)
 Introduction to
Biochemistry
• Science concerned with chemical basis of life
• Science concerned with the chemical constituents
of living cells and with the reactions and processes that they undergo

4
IT IS RELATED TO ALL OTHER
SCIENCES VIZ;
Physiology
Immunology
Pharmacology
Microbiology

Pathology

5
Biochemistry studies have illuminated many
aspects of health & disease
The study of various aspects of health & disease has
opened up new areas of biochemistry

Normal biochemical processes are the basis of

health

6
 A strict biochemistry viewpoint
about health :
• The situation in which all of the many
thousands of intra & extra cellular reactions
that occur in the body are proceeding at the
rates balanced with the individual’s maximal
survival in the physiological state
 Biochemistry

Nucleic
protein lipid Carbohydrates
acid

Genetic arterioscler- Diabetes

Sickle osis mellitus
disease cell
anemia Medicine 8
 What are the goals of a
medical biochemistry
course?
• Learning and appreciating biochemistry;
• course specific goals are the following:
– To understand how large molecules are
synthesized and function (DNA, RNA, proteins)
– To understand how energy is stored, retrieved and
generated (metabolism)
– To understand the future of diagnostic medicine
(human genetics and cancer)
– To understand biological processes at the
molecular level
 ASSESMENT
METHODS
• CLASS TESTS & VIVA VOCE
• TUTORIAL DISCUSSION
• PRESENTATIONS BY STUDENTS
• CHARTS PREPARATION
• ASSIGNMENTS
• BIOCHEMISTRY COURSE
CONTENT
• INTRODUCTION
• BIOCHEMISTRY OF CELL
• BIOLOGICAL MEMBRANES
• BIOCHEMISTRY OF BODY FLUIDS
• CHEMISTRY OF CARBOHYDRATES
• PROTEINS
• AMINO ACIDS
• NUCLEOTIDES AND NUCLEIC ACIDS
• LIPIDS
• ENZYMES
• PORPHYRINS AND HAEMOGLOBIN
• VITAMINS
• DIGESTIVE TRACT
• MINERALS AND TRACE
• ELEMENTS
•COURSE CONTENTS FOR
PRACTICALS
•INTRODUCTION TO LAB FACILITIES
Rules to be observed while working
Precautions and emergency first aid measures for
working in medical laboratory
Introduction of various techniques, equipments
Solutions, definition and preparation of various types-
of solutions
•PHYSICOCHEMICAL ASPECTS
pH metery
Demonstration of effects of solutions of different-
osmotic
concentrations on RBC
Demonstration of adsorption
Observation of effects of various compounds on-
surface tension
•QUALITATIVE ANALYSIS OF
CARBOHYDRATES
•Molisch's test
•Iodine test for polysaccharides
•Reducing action of sugars in alkaline medium
•Benedicts test
•Fehling's test
•Barfoed's test. Reducing action of sugars in-
•acidic medium
•Detection of keto sugars
•Selivanoff’s test
•Hydrolysis of starch by mineral acids
•Hydrolysis of sucrose by mineral acids
•Scheme for detection of unknown CHO
•CHEMISTRY OF LIPIDS
Solubility of fats
Test for identification of cholesterol
Emulsification of fats
Acrolein formation
•CHEMISTRY OF PROTEINS
Colored reaction
Precipitation of protein by different reagents
Effect of strong acids
Precipitation of proteins by salts of heavy-
metals
Precipitation of proteins by acid or alkali-
reagents
Precipitation by alcohol
Fractional precipitation of proteins with-
ammonium sulphate
Scheme for detection of unknown proteins
•URINANALYSIS
Analysis of normal urine sample
Analysis of abnormal urine sample

•ESTIMATION OF BLOOD GLUCOSE LEVEL

•ESTIMATION OF HEMOGLOBIN
 RECOMMENDED BOOKS
• HARPER’S ILLUSTRATED
BIOCHEMISTRY
• LIPPINCOT’S REVIEW OF
BIOCHEMISTRY
• JAYPEE’S BIOCHEMISTRY
• LEHNINGER PRINCIPLE’S OF
BIOCHEMISTRY