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PRINCIPLE
Semiquantitative method to detect the presence of glucose
and other reducing substances in feces.
Cupric sulfate is converted to cuprous oxide when copper
sulfate reacts with reducing substances.
The resultant color, which varies with the amount of
reducing substances present, ranges from blue through
green to orange.
Sodium hydroxide provides an alkaline medium necessary
for the reaction to occur. Heat required is furnished by the
reaction of sodium hydroxide with water and with citric acid.
Citric acid and sodium carbonate aid in dissolving the
tablet.
PROCEDURE:
A. Quality Control Testing
1. Drop 5 drops of the Level 1 control into one test tube and 5 drops
of Level 2 control into another test tube.
NOTE: If more than one specimen is being tested, the test
tube should be identified to prevent clerical errors from occurring.
Also if a pipette is used, then a pipette should be used for all steps.
If a syringe is used, then a syringe should be used for all steps.
2. Using a pipette or syringe in an upright position, drop 10 drops of
distilled or sterile water into each test tube.
3. Remove a tablet from Clinitest bottle (do not remove prior to this
time) for each tube.
B. Patient Testing
1. Collect feces following the appropriate collection procedure.
2. In a container, place 1 volume of stool.
3. Add 2 volumes of distilled/sterile water to the container.
4. Mix thoroughly.
5. Transfer 15 drops of this mixture to a clean test tube.
6. Remove tablet from Clinitest bottle (do not remove prior to
this time).
7. Drop tablet into test tube. DO NOT touch bottom of tube
- HOT!
8. Watch while complete boiling reaction takes place. DO NOT
shake
test tube during boiling, or for the following 15 seconds after
boiling has stopped.
REPORTING RESULTS
Expected Results:
The presence of 0.25mg/dl (1/4%) or less
is considered normal.
The presence of 0.25 to 0.50mg/dl is
considered suspicious.
The presence of 0.5.mg/dl or greater is
considered abnormal.
Color Block Designations:
Negative, 1/4% (0.25), 1/2% (0.50), 3/4%
(0.75), 1%, 2% or more.