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Repairing
Biomaterial
PRESENTATION
BY
Er Parampreet Kaur
Introduction
Cracking
of Concrete is an inevitable
phenomenon.
Remediation of already existing cracks
has been the subject of research from
many years.
The various products such as structural
epoxy resins, other synthetic mixtures
are used as a filling agents for repairing
concrete.
The use of Epoxy resins is neither Ecofriendly nor safe for human health.
Objectives
To
Efficency in Different
medium Concentrations .
Bacterial inherent ability to
precipitate calcite continiously
Bacteria Survival Duration in
Specimen.
Reaction Tendency of Different
Types of Bacteria under Different
medium Concentrations.
Formation Mechanism
(B.Pasturii)
B . Pasturii is formed by the
mechanism of oxidative
determination of amino acids and the
nutrients required for its formation
are:Trypticase
Yeast extract
Tricine
Agar solution
Glutamic Acid
Formation Mechanism
(B.Subtiles)
B . Subtiles is formed by the
mechanism of Hydrolysis of Urea
and the nutrients required for its
formation are:Urea
Calcium salts
Ammonium chloride
Sodium Bicarbonate
XRD Analysis
XRD
Microstructure
Examinations:It
Rod Shaped
Impressions
Developing Calcite
Crystals
Bacterial
Endospores
Durability Comparison
100
100
100
100
100
91.81
86.11
92.05
87.52
Durability Factor.
80
60
0 cycles
40
300 cycles
20
0
Control1
Control2
Bacteria1
Bacteria2
Mix Designation
Compressive Strength
Comparision
Bacteria
Specimen
Compressive
Strength
B. Pasturii
30
B. Subtiles
33
B. Sphaericus
28
B. Cohnii
29
CONCLUSIONS
It was found that beams with micro cracks remediated with a bacterial
(Bacillus pasteurii) concentration of 8.6108 cells/ml regained 81.97% of
its original strength.
Higher concentrations reduced the regaining strength of the beams.
It was found that all the specimens with bacteria formed a layer of calcite
at the surface, thus improving its impermeability and its resistance to
alkaline environment, sulfate attack, deicing chemicals and freeze-thaw.
Scanning Electron Microscope was used to document the role of
bacteria in improving the durability aspects of concrete.
Thus it can be concluded that cracks remediated with bacteria can
improve the strength and the durability of the structure.
Conclusion
ACKNOWLEDGEMENTS
BThe
. Subtiles
is foundacknowledge
to be most
forthe
authors gratefully
theeffective
support formicrobiome
the research by
the
crack
remediation
it remediates
the CMS-9802127.
crack easily The
National
Science
Foundationasunder
contract number
and
in development
of strength
in early age.
SEMhelps
analyses
were conducted
in the Engineering
and Mining
Experiment Station of the South Dakota School of Mines and
Technology under the guidance of Dr. Edward Duke.