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Methods of Sterilisation
by oxidation effects
FLAMING
Inoculation loop
INCINERATION
proteins.
Pasteurisation
(HOLDER METHOD)
At 72oC for 15-20 Sec. Rapid cooling to 13oC
(FLASH PROCESS)
Water Bath
Inspissator
Inspissator
Arnold)
Consists of a Tin cabinet
Has a conical lid to enable the drainage of
condensed steam
Perforated tray above ensures materials are
surrounded by steam.
For routine sterilization exposure of 90 mins is
used
Steam Sterilizer
materials to be sterilised.
The lid is provided with a Pressure gauge, to measure the
pressure
A safety valve is present to permit the escape of steam
from the chamber
Articles to be sterilised are placed in the basket provided
Sterilisation is carried out under pressure at 121 for 15
mnts.
osmotic pressure.
RADIATION
Three types of radiations are used
NON IONISING
IONISING
MICROVAVE
Forms of Radiation
Chemical Methods of
Microbial Control
Rarely used today because it is a skin irritant and has strong odor.
Used in some throat sprays and lozenges.
Acts as local anesthetic.
Cresols: Derived from coal tar (Lysol).
Biphenols (pHisoHex): Effective against gram-positive staphylococci and
streptococci. Used in nurseries. Excessive use in infants may cause
neurological damage.
Betadine
Isodine
H+
+ Cl- + HOCl
Hypochlorous acid
machines,respirators,sutures,dental equipments,
books and clothing.
Also used to sterilise Glass, metal and paper
surfaces,plastics, oil,some foods and tobacco.
DYES
Two groups of dyes are used
Aniline dyes
Acridine dyes
ANILINE DYES
Are Brilliant green, Malachite green &
Crystal violet
Active against Gram positive bacteria
No activity against tubercle bacilli
ACRIDINE DYES
Acridine dyes in use are orange in colour
Effective against Gram positive than Gram
negative
Important dyes are Proflavine,
Acriflavine,Euflavine
FORMALDEHYDE GAS
BETA PROPIOLACTONE
Used in fumigation
For sterilisation 0.2% BPL is used
Has a rapid biocidal activity
Very effective against viruses
METALLIC SALTS
The salts of silver, copper and mercury are used
as disinfectants.
Act by coagulating proteins
Marked bacteriostatic, weak bactericidal and
Mechanical
Methods of
Microbial Control
Sterilisation by filtration
Filtration helps to remove bacteria from heat
labile liquids such as sera and solutions of
sugar, Antibiotics.
The following filters are used
Candle filters
Asbestos filters
Sintered glass filter
Membrane filters
CANDLE FILTER
CANDLE FILTERS
Widely used for purification of water
Two types
(a) Unglazed ceramic filter Chamberland
filter
(b) Diatomaceous earth filters Berkefeld filter
SEITZ FILTER
a filter made of compressed
asbestos fibers used in
microbiological work to
sterilize fluids such as
serum and to filter off
bacteria
ASBESTOS DISCS
ASBESTOS FILTER
Disposable single use discs
High adsorbing tendency
Carcinogenic
MEMBRANE FILTER
MEMBRANE FILTERS
Made of cellulose esters or other polymers
Uses
Water purification & analysis
Sterilization & sterility testing
Preparation of solutions for parenteral use
Review
Sterilization
The killing or removal of all microorganisms
in a material or on an object.
Disinfection
The reduction of the number of pathogenic
microorganisms to the point where they
pose no danger of disease.
Review
Antiseptic
A chemical agent that can safely be used
externally on living tissue to destroy
microorganisms or to inhibit their growth
Disinfectant
A chemical agent used on inanimate objects to
destroy microorganisms. Most disinfectants
do not kills pores.
Review
Sanitizer
A chemical agent typically used on foodhandling equipment and eating utensils to
reduce bacterial numbers so as to meet
public health standards. Sanitization may
simply refer to thorough washing with only
soap or detergent.
Review
Bacteriostaticagent
An agent that inhibits the growth of bacteria.
Germicide
An agent capable of killing microbes rapidly;
some such agents effectively kill certain
microorganisms but only inhibit the growth of
others.
Review
Bactericide
An agent that kills bacteria. Most such agents do
not kill spores.
Viricide: An agent that inactivates viruses.
Fungicide: An agent that kills fungi.
Sporocide: An agent that kills bacterial
endospores or fungals pores.