Documente Academic
Documente Profesional
Documente Cultură
1.Karimatul Aini
2.Sylvia Anggraeni
3.Retno Dwi P.
4.Nurvita Wahyu K.
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t
i
n
U
Metric
Light Properties
Wavelength
Polarity
Light is a wave
Waves can be
added
+
Light
Properties
Resolution
Wavelength/Resolution
Interaction
Light Properties
Reflection
Transmission
Light Properties
Absorption
Refraction
bending
Light Microscopy
Types
Compound
Bright Field
10
Oil immersion
without
Microscopy Dark
Field
12
Microscopy Phase
Contrast
Dual Beam
13
Phase contrast
Microscopy DIC
Differential
Interference
Contrast
15
DIC differential
interference contrast
Similar to phase contrast, but input light is polarized
16
Microscopy
Fluorescence
Ultraviolet light
flourescein
17
Advantages of
fluorescence
Can use specialized chemical probes that target specific features
and then tag with fluorescent dyes
Microscopy
Confocal
Confocal
Confocal microscopy
Allows 3 dimensions
20
Antibody labeling
21
Microscopy Imaging
Digital
22
Fig. 2-15
23
Electron Microscopy
Transmission
(TEM)
Scanning (SEM)
Scanning
Tunneling (STM)
24
TEM
25
Electron Microscopy
Images
26
Microscopy
Techniques
Wet Mounts
Smears
Staining
27
Dry in air
I. Preparing a smear
100
Slide
III. Microscopy
Oil
Staining cells
contrast
increases
yeast
Cheek cell
29
Common stains
Safranin (*basic, + charge)
red
Crystal violet
30
31
Differential stains
Use multiple dyes or dyes that interact with organisms
differently.
32
Gram Stain
33
34
Gram Stain
35
36
37
Acid Fast
38
Acid Fast of
Mycobacterium
tuberculosis
39
40
Negative Stain
41
42
Flagellar Stain
Salmonella typhimurium
43
Endospore Stain
Used on spore
forming bacteria
such as Bacillus
sp.
Malachite green
stains spores
44
45
46
WASSALAMUALAIK
UM
THANK YOU
47