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  • Wet Mount

    Încărcat de

    DR VARSHA A SINGH
    283 vizualizări37 pagini
    This document discusses the preparation and use of wet mount microscopy to examine various clinical specimens for infectious agents and cellular elements. Wet mounts are prepared by suspending a liquid sample in saline between a slide and coverslip to allow microscopic examination of organism movement and morphology. Specimens that can be examined include urine, stool, cerebral spinal fluid, bronchial lavage, pleural fluid, liver abscess aspirate, vaginal swabs, and synovial fluid from various sites of infection or inflammation. Key cellular elements and microbes that may be observed for different specimen types are outlined.

    Descriere originală:

    dr khusboo Gautam

    Titlu original

    Wet mount

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    This document discusses the preparation and use of wet mount microscopy to examine various clinical specimens for infectious agents and cellular elements. Wet mounts are prepared by suspending a liquid sample in saline between a slide and coverslip to allow microscopic examination of organism movement and morphology. Specimens that can be examined include urine, stool, cerebral spinal fluid, bronchial lavage, pleural fluid, liver abscess aspirate, vaginal swabs, and synovial fluid from various sites of infection or inflammation. Key cellular elements and microbes that may be observed for different specimen types are outlined.

    Drepturi de autor:

    © All Rights Reserved
    Descărcați ca PPTX, PDF, TXT sau citiți online pe Scribd
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    283 vizualizări37 pagini

    Wet Mount

    Încărcat de

    DR VARSHA A SINGH
    This document discusses the preparation and use of wet mount microscopy to examine various clinical specimens for infectious agents and cellular elements. Wet mounts are prepared by suspending a liquid sample in saline between a slide and coverslip to allow microscopic examination of organism movement and morphology. Specimens that can be examined include urine, stool, cerebral spinal fluid, bronchial lavage, pleural fluid, liver abscess aspirate, vaginal swabs, and synovial fluid from various sites of infection or inflammation. Key cellular elements and microbes that may be observed for different specimen types are outlined.

    Drepturi de autor:

    © All Rights Reserved
    Descărcați ca PPTX, PDF, TXT sau citiți online pe Scribd
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    din 37

    Wet Mount

    MODERATOR:
    Dr.Varsha A Singh
    PRESENTED BY :
    Dr.Khushboo gautam

    Introduction

    Defination:
    liquid specimen/ sample suspended in saline

    slide and the cover slip by surface tension.


    use:
    view microscopic organisms

    to study their movement and morphology.

    Preparation of wet mount

    Specimens to be examined
    Urinary tract infection-Urine
    Gastro-intestinal infection-Stool, peritoneal fluid
    Central nervous system infections-CSF
    Respiratory tract infection-BAL, Pleural fluid
    Female genital tract infection-Vaginal swab
    Bone and joint infections-Synovial fluid

    Urinary tract infection


    Elements found in urine
    Cells-RBC ,WBC, Epithelial &neoplastic cells
    Infectious agents-Yeast , Bacteria
    Casts
    Crystals

    RBC
    >5RBC/HPF-hematuria
    Fresh RBC-Lower UT
    Haemorrhage
    Inflammation
    Trauma
    Crenated RBC
    Dehydration
    Diarrhea
    Heart failure
    Glycosuria

    WBC
    >5WBC/HPF-In uncentrifuged urine
    Pyuria

    Squamous epithelial cells

    Vaginal contamination

    Transitional epithelial cells

    Bladder infection

    Neoplastic cells

    Renal tubular epithelial cells

    Renal carcinomas
    Acute tubular necrosis
    Heavy metal intoxication

    Yeast

    Bacteria

    Egg of schistosoma haematobium

    Cellular cast
    RBC Cast

    Glomerulonephritis
    Renal infarction
    urinary tract injury
    subacute bacterial endocarditis

    WBC Cast

    pyelonephritis
    Acute allergic interstitial nephritis

    A cellular Cast
    Hyaline cast

    Low urine flow


    Dehydration
    Vigorous exercise

    Granular Cast

    Acute Renal failure


    Acute tubular necrosis

    Waxy cast

    Advanced Kidney disease


    Chronic renal failure

    Crystals
    Triple phosphate

    Chronic cystitis
    Chronic pyelitis

    Cholesterol crystals

    Nephritis
    Nephrotic syndrome

    Calcium oxalate dihydrate crystals

    Diabetes mellitus
    Chronic liver disease

    Tyrosine

    Severe liver disease

    Drug

    Sulfa drugs

    Stool

    Gastrointestinal tract infection

    Bacterial

    parasitic

    Bacterial
    Diarrhea

    Dysentery

    pus cells
    Pus cells

    Pus cells

    RBC in rouleax pattern

    Vibrio-darting motility

    Shigella- dead background

    parasitic
    Saline
    Trophozites/adult worms

    Iodine
    cysts & ova

    Giardia lamblia
    Hookworm egg

    Ancylostoma duodenale

    stercoralis strongyloides

    Enterobius vermicularis

    Ascaris lumbricoides

    Charcot leyden crystals

    candida

    Antibiotic induced diarrhoea

    Peritoneal fluid
    Ascites

    WBC

    BRONCHO ALVEOLAR LAVAGE

    pus cell
    epithelial cells
    Honeycomb" structures
    -Pneumocystis carinii

    BAL-India ink

    Cryptococcus neoformins

    Strept. pneumoniae

    Candida albicans

    Pleural fluid

    Aspergillus fumigatus

    Lung abscess

    Tapping or draining of fluid

    Trophozoite
    of E.histolytica

    Liver abscess
    Aspirate from a space-occupying hepatic lesion

    Trophozoite
    Of E.histolytica

    CSF

    Viral infection-2-4WBC/HPF

    Pyogenic bacterial infection-10WBC/HPF

    Tuberculosis and mycoses->5WBC/HPF

    WBC(monocyte)

    Lymphocytes under methylene blue


    preparation

    candida

    India ink preparation

    C.neoformins

    Strept. pneumoniae

    Naegleria fowleri

    Acanthamoeba

    vaginal swab

    Motile trophozoites of trichomonas vaginalis


    saline preparation

    Vulvovaginal candidiasis

    pseudo hyphae or budding yeast -saline


    wet preparation

    Bacterial vaginosis

    Presence of >20% per HPF of clue cells on


    wet mount examination

    synovial fluid

    Non inflammatory

    -1 to 2 WBC/HPF

    Inflammation or infection

    ->20 WBC/HPF

    Septic
    - >100WBC/HPF
    (also Rheumatoid arthritis,gout,pseudogout)

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