Mary K.

Campbell
Shawn O. Farrell
http://academic.cengage.com/chemistry/campbell

Chapter One
Biochemistry and the Organization of Cells

Paul D. Adams University of Arkansas

Some Basic Themes

All living things make use of the same types of biomolecules,
and all use energy
as a result, all living things can be studied using the
methods of chemistry and physics
The fundamental similarity of cells of all types makes it
interesting to speculate on the origins of life
both cells and the biomolecules of which they are made
must have arisen ultimately from very simple molecules,
such as H2O, CH4, CO2, NH3, N2, and H2

Field of Biochemistry draws many disciplines

allows us to answer questions related to molecular nature
of life

Biomolecules
Organic chemistry: the study of the compounds of
carbon
the cellular apparatus of living organisms is made up
of carbon compounds
biomolecules are part of the subject matter of organic
chemistry
the reactions of biomolecules can be described by the
methods of organic chemistry

The experiment of Friedrich Whler in 1828

Levels of Structural Organization in the

Human Body

Biomolecules (Contd)
Functional group: an atom or group of atoms that shows
characteristic physical and chemical properties

ATP and The Reactions for its Formation

Origins of Life
The big bang theory
all matter was originally confined in a very small space
as the result of an explosion, it started to expand with great
force; temperature approx. 15 x 109 K
the average temperature of the universe has been decreasing
ever since
in the earliest stages of the universe, the only elements present
were H, He, and Li
other elements formed by
thermonuclear reactions in stars
explosions of stars
the action of cosmic rays outside the stars

Relative Abundance of Important Elements

Biomolecules (Contd)
Gases present in the atmosphere of the early earth included
NH3, H2S, CO, CO2, CH4, N2, H2, and H2O but not O2
Experiments have demonstrated that important biomolecules,
such as proteins and nucleic acids, could have arisen under
abiotic (nonliving) conditions from reactions of these simple
compounds
in the earths oceans
on the surface of clay particles

Biomolecules (Contd)
Living cells include very large molecules, such as proteins,
nucleic acids, polysaccharides, and lipids
these biomolecules are polymers (Greek: poly + meros, many +
parts)
they are derived from monomers (Greek: mono + meros, single +
part)
--amino acids --> proteins
--nucleotides --> nucleic acids
--monosaccharides --> polysaccharides
--glycerol and 3 fatty acids --> lipids

Informational Macromolecules

Biomolecules (Contd)
Enzymes: a class of proteins that are biocatalysts
the catalytic effectiveness of a given enzyme depends on its
amino acid sequence

Genetic code: the relationship between the nucleotide

sequence in nucleic acids and the amino acid sequence in
proteins
theories of the origin of life consider how such a coding system
might have arisen

Biomolecules (Contd)
Which came firstthe chicken or the egg?
catalytic activity associated with proteins
coding associated with nucleic acids
It has been discovered recently that certain types of RNA have
catalytic activity and are capable of catalyzing their own further
processing (See Figure 1.7 p.12)
RNA is now considered by many scientists to have been the
original coding material
it still serves this function in some viruses

The RNA World

The appearance of a form of RNA capable of coding
for its own replication was the pivotal point in the origin
of life
This original RNA both encoded for and catalyzed its
own replication
In time, this system evolved to encode for the
synthesis of protein catalysts
Even later, DNA became the primary genetic material,
and RNA took on only an intermediary role in the
synthesis of proteins

Stages in the Evolution of Self-replicating

RNA Molecules

Theories on the Origin of Life

A key point in the development of living cells is the
formation of membranes that separate cells from their
environment
Some theories of the origin of life focus on proteins
according to one model, proteinoids aggregated to
form microspheres
Double-Origin theory: the development of a coding
system and the development of catalysis came about
separately
a combination of the two later in time produced life as
we know it.

Prokaryotes and Eukaryotes

Prokaryote: Greek derivation meaning before the
nucleus
single-celled organisms
include bacteria and cyanobacteria

Eukaryote: Greek derivation meaning true nucleus

contain a well-defined nucleus surrounded by a

nuclear membrane
can be single celled, such as yeasts and Paramecium,
or multicellular, such as animals and plants

Comparison of Prokaryotes and Eukaryotes

A Comparison of a typical animal cell, plant

cell, and prokaryotic cell

Important organelles
listed in table 1.3

Comparison of Prokaryotes and Eukaryotes

Five Kingdoms, Three Domains

5-kingdom system takes into account differences
between prokaryotes and eukaryotes
Provides classification for eukaryotes that are neither
plants nor animals
Kingdoms are: Monera, Protista, Fungi, Plantae, and
Anamilia

Five Kingdoms, Three Domains

What is source of energy in cells?

Light from the sun is the ultimate source of energy for
all life on earth
photosynthetic organisms use light energy to drive the
energy-requiring synthesis of carbohydrates
non-photosynthetic organisms consume these
carbohydrates and use them as energy sources

The energetics of a chemical reaction

if the change in free energy is negative (free energy
decreases), the reaction is spontaneous as written
if the change in positive (free energy increases), the
reaction will not occur as written unless energy is
supplied from an external source

How are energy changes measured?

Thermodynamics- branch of science that answers questions
about processes that are energetically favorable

Spontaneity in biochemical reactions

Free Energy of a System
G < 0 spontaneous exergonic- energy released
G= 0 Equilibrium
G > 0 Nonspontaneous endergonic- energy required

Life and Thermodynamics

G=H-TS
H is heat of a reaction at constant pressure
S is the change in entropy
G is the change in free energy
T is the temperature