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BINE ATI VENIT LA

CATEDRA DE
FIZIOPATOLOGIE!

Asist. Dr. ORASAN MEDA


SANDRA

Vom parcurge in acest


semestru 14 lucrari practice:
20% din ele .. 3 absente posibile,
dar care
a) trebuie recuperate!
b) trebuie platite, daca
nu aveti motivare de la Decanat

NOTA FINALA
EXAMEN SCRIS .. 70%
EXAMEN PRACTIC .. 20%
ACTIVITATE DIN SEMESTRU ..10% =
= o prezentare powerpoint de 10-15
slides, cu durata: 10 minute, un subiect
legat de cursuri sau de lucrarile practice.
Puteti alege dintr-o lista de titluri
posibile, care va fi trimisa prin e-mail.

TITLURILE LUCRARILOR
PRACTICE
Studiul actiunii agentilor chimici asupra
organismului
Studiul actiunii agentilor fizici asupra organismului
Inflamatia si reactia febrila experimentala
Algoritm de diagnostic al tulburarilor hemostazei
(2 sedinte)
Algoritm de diagnostic al tulburarilor functiei
cardio-vasculare ( 3labs)
Algoritm de diagnostic al anemiilor
Algoritm de diagnostic al tulburarilor functiei
respiratorii
Algoritm de diagnostic al tulburarilor
metabolismului
- lipidic
- glucidic
- proteic

Lucruri necesare
1) Carte
LUCRARI PRACTICE DE FIZIOPATOLOGIE
STUDENTI DE ANUL III Editia a III sub
redactia Prof Dr Luminita Plesca Manea
Tipografia UMF- 1998
2) Caiet / foi indosariate
- protocolul experimentului
- cazurile clinice
3) Vaori normale pentru investigatiile de
laborator

EXPERIMENTUL
O metoda des folosita in
fiziopatologie
IN VIVO: pe animale experimentale
IN VITRO: pe culturi de celule

PROTOCOLUL
EXPERIMENTAL
1.
2.
3.
4.
5.
6.

Scop
Materiale
Metoda
Rezultate
Discutii
Concluzii

Inainte & dupa


experiment evaluam
parametrii biologici

1. Frecventa respiratorie
2. Frecventa cardiaca
3. Tonusul muscular (Crescut, scazut,
contractii, frison)
4. Temperatura rectala
5. Pupile (mioza/midriaza)
- comportament
- stare de veghe/
somn
- culoarea tegumentului
- salivatie / secretie lacrimala

FACTORI CHIMICI

FIRST EXPERIMENT
1. TASK: Observation of the organisms response to
barbiturates
2. MATERIALS: RAT, i.m. phenobarbital 0,2g/kg body.
3. METHOD: before and after injection (after 20 min)
record: heart rate, respiratory rate, rectal
temperature, muscular tone, pupils diameter and
reflexes.
4. RESULTS: sleepy/deep sleep, decreased heart,
respiratory rate, rectal temperature, muscle tone,
reflexes of pupils and miosis
5. DISCUTION: barbiturates = GABA receptor
agonists and inhibit the CNS from confusion to
death (coma in two steps)
6. CONCLUSION: Poisoning with barbiturates
produces: hipnotic, sedative, tranquillizer,
anticonvulsive effect.

SECOND EXPERIMENT
1.

TASK: Observation of the organisms response to


organophosphates
2. MATERIALS: RAT,s.c. neostigmine 0,01%, 1ml/100g body.
3. METHOD: before and after injection (after 10 min) record: heart
rate, respiratory rate, rectal temperature, muscular tone, pupils
diameter and reflexes. Atropine 0,1% is injected to prevent death.
4. RESULTS:
After
ATROPINE:
M1 effects: nausea, dyspnoea, miosis
mydriasis
M2 effects: bradicardia, hypotension
tahycardia
M3 effects: sweating, salivation, shadding tears
hiposalivation
N effects: twitching, fasciculations
CNS: anxiety, convusions, coma
5. DISCUTION: organophosphates = causes accumulation of
Acetylcholine in the synapses and produces M si N effects
6. CONCLUSION: organophosphates are absorbed by skin, lung,
gastro-intestinal tract, distributed in the tissues, slowly eliminated
by the liver.

THIRD EXPERIMENT
1. TASK: Observation of the organisms response to ethanol
2. MATERIALS: Sobolan, i.p. 1ml/ 100g body weight, alcool
etilic 33%
3. METHOD: Before and after 5-10min from the
administration have to be assessed: respiratory rate,
heart rate, rectal temperature, musculature tone, pupils
diameter and pupils reflex.
4. RESULTS:
Uncoordinatedly movements and reduced muscular tone
Redness of the ears, tail and paws, Agitation/ sleep,
Mydriasis
Rise of the respiratory and heart rate, rectal
temperature
Mydriasis.
5. DISCUTION: Ethanol is a CNS depressant that decreases
activity of neurones. The mechanisms of action of
alcohol on nervous tissues are not fully understood
because even modest doses simultaneously change
many neurotransmitters and increase the fluidity of
neuronal cell membranes.

FACTORI

FIZICI

FOURTH EXPERIMENT
1.
2.
-

3.
4.
5.
6.

TASK: Observation of the organisms response to hypothermia


MATERIALS: 2 RATS, at 4 degrees, for 30 minutes
TEST: i.m. phenobarbital 2%
plegomaxin 0,5% + Romergan
Flaxedyl 0.04% ( the temperature regulating
system is blocked)
CONTROL:
METHOD: before and after injection, record: heart rate,
respiratory rate, rectal temperature, muscular tone, skin color
RESULTS:
TEST: decreased heart rate, respiratory rate, rectal
temperature, muscle tone, cyanosis
CONTROL: tahycardia, shivering, normal rectal temperature,
restlessness
DISCUTION: when the temperature regulating system is blocked
or the physiological mechanism is outrun, hypothermia
appears due to low environmental temperature.
CONCLUSION: Lowering of body temperature depresses organs
and system functions.

HYPOBARISMUS

FIFTH EXPERIMENT
1. TASK: Observation of the organisms response to
hypobarismus
2. MATERIALS: 1 RATS, in chamber with vacuum pump
3.
METHOD: before and after , record: behavior, heart
rate, respiratory rate, muscular tone, skin color
4. RESULTS: restlessness, tahypneea,
tachycardia(irregular), cyanotic skin, reduced muscle
tone.
5. DISCUTION: at altitude the hypoxia increases the
respiratory rate as compensatory and signs appear:
mental fatigue, headache, fatigue, convulsions, coma
6. CONCLUSION: the effects of hypobarismus lead to:
hypoxic hypoxia, decreases of gases solubility in the
organisms tissues, dilatation of natural cavities
volumes.
Compensatory mechanisms: increased heart rhythm,
respiratory rate, cardiac debit, RBC mobilization from
storage organs (spleen contraction)