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GROUP 4
A.)WHAT
IS
THE
PRINCIPLE
INVOLVED IN THE SELIWANOFF'S
TEST?
When heated, ketoses are more rapidly
dehydrated than aldoses.
When Seliwanoff's reagent is added to a solution
containing ketoses, a red color is formed rapidly
indicating a positive result. When added to a
solution containing aldoses, a slower forming light
pink or faint red is observed instead.
PROCEDURE:
TEST TUBE 1
TEST TUBE 2
FRUCTOSE - POSITIVE
FRUCTOSE
SUCROSE
GLUCOSE
KETOSE
KETOSE, ALDOSE
ALDOSE
Red
Pale red
Yellow
BEFORE HEATING
Negative Results
no red color appearing in the solution
All other sugars except fructose and sucrose gives
negative result with seliwanoffs reagent.
Aldose sugars have an aldehyde group, while ketose
have a ketone group. So if heated, ketose is faster
dehydrated than aldose.