TOPIC :

MECHANISM OF
ENZYME ACTION

CONTENT
1

LEARNING
OUTCOME

INTRODUCTION

PRIOR
KNOWLEDGE

DEFINITION OF
ENZYME

MECHANISM OF
ENZYME ACTION

ASSESSMENT

LEARNING OUTCOME

4.10 :
Explain the mechanism of
enzyme action

INTRODUCTION
Do you realize the
term of COENZYME
on detergent
products?

PRIOR KNOWLEDGE
RECALL !!!
Maltose + Water

Maltas
e

Glucose + Glucose

ENZYME maltase BREAK

DOWN maltose into 2
molecules of glucose

Prior knowledge
Enzyme
s

Cloth

Stains

How the stains have been

removed from the cloth?

DEFINITION OF ENZYME

Enzymes are biological

catalyst that direct or guide
almost all cellular reactions.

MECHANISM OF ENZME
ACTION
LOCK AND KEY
HYPOTHESIS

MECHANISM OF ENZME
ACTION
Product

Product
Enzyme-substrate
complex

Enzyme

EXPLANATIO
N

EXPLANATIO
N

Substrate

EXPLANATIO
N

MECHANISM OF ENZME
ACTION

A specific substrate molecule

arrives at the active site of the
enzyme molecule.

MECHANISM OF ENZME
ACTION

The substrate molecule binds to

the active site to form an
enzyme-substrate complex
which is very unstable.

MECHANISM OF ENZME
ACTION
Product molecules leave the
active site of the enzyme.
The enzyme is free to take
another substrate molecule into
its active site to catalyse
another reaction.

MECHANISM OF ENZME
ACTION

Which substrate can bind to active site of

enzyme sucrase?
Sucrose can bind to
active site of sucrase

Sucrose

Sucrase

Maltose

Maltose CANNOT bind

to active site of sucrase

Enzymes
have
specific
sites
called
active site
which bind
to specific
substrates

assessment

Question 1

Question 2

Question 3

Question 4

Question 1
Fill the blank with the correct answer.
1. A specific ______________ molecule arrives
at the active site of the ______________ molecule.
2. The substrate molecule binds to the active site of the
enzyme to form an ______________.

Product
enzymesubstrat
e
complex
enzyme

3. The enzyme is free to take another substrate molecule

into its ______________ to catalyse another reaction.
4. ______________ molecules leave the active site of the
enzyme.

active
site
substrat
e

Question 2

Which of the following shows the characteristic of

enzymes?
A. Enzyme reaction is specific
B. Enzyme reaction is influenced by pH
C. Enzyme reaction is influence by
temperature
D. The structure of the enzyme change at
the end of the reaction.

Question 3
Protea
se

Dissolve
starch stains
on cloth

Amylas
e

Ripening
cheese

Lipase

Dissolve
protein stains
on cloth

Question 4
Enzyme is used to wash away the blood stain on a
shirt.
Explain how the enzyme acts on the blood stain at a
maximum rate.
(3 MARKS)

ANSWER :
Blood is made up from proteins. Protease
in detergent will break down protein into
amino acids. This reaction occur

THANK YOU .