Documente Academic
Documente Profesional
Documente Cultură
Gene to Protein
History
For years the source of heredity was
unknown. This was resolved after
numerous studies and experimental
research by the following researchers:
Fredrick Griffith
He was studying effects of 2 strains of an
infectious bacteria, the "smooth" strain was
found to cause pneumonia & death in mice.
The "rough" strain did not. He conducted the
following experiment
Griffith Experiment
Bacteria Strain injected into
mouse
Result
Smooth Strain
Mouse dies
Rough strain
Mouse Lives
Mouse lives
*MOUSE
DIES*
Hershey-Chase
1 virus was "tagged" with
32P on it's DNA
The other was "tagged"
35S on it's protein coat.
Researchers found the
radioactive P in the
bacteria, indicating it is
DNA, not protein being
injected into bacteria.
DNA Structure
Nucleotides
DNA is formed by
Nucleotides
These are made
from 3 components
1.
2.
3.
A 5-Carbon Sugar
A Nitrogenous base
A Phosphate group
Nucleotide types:
Adenine (A)
Cytosine (C)
Guanine (G)
Thymine (T)
Base Pairing
Each "Rung" of the DNA "staircase" is formed by the linking of 2
Nucleotides through Hydrogen Bonds.
These Hydrogen bonds form only between specific Nucleotides.
This is known as Base Pairing. The rules are as follows:
Adenine (A) will ONLY bond to Thymine (T) (by 2 hydrogen bonds)
Cytosine (C) will ONLY bond to Guanine (G) (by 3 hydrogen bonds)
DNA Replication
DNA Replication
RNA Transcription
It is much smaller
It is single-stranded
It does NOT contain Thymine,
but rather a new nucleotide called
Uracil which will bind to Adenine.
RNA Transcription
mRNA
Each three
Nucleotide
sequence in an
mRNA strand is
called a "Codon"
Each Codon codes
for a particular
amino acid.
The codon
sequence codes for
an amino acid using
specific rules.
These specific
codon/amino acid
pairings is called the
Genetic Code.
tRNA
There is a special form of
RNA called Transfer RNA or
tRNA.
Each tRNA has a 3
Nucleotide sequence on one
end which is known as the
"Anitcodon"
This Anticodon sequence is
complimentary to the Codon
sequence found on the strand
of mRNA
Each tRNA can bind
specifically with a particular
amino acid.
Ribosome
Consists of two
subunits
Large subunit
Small subunit
Serves as a template
or "work station"
where protein
synthesis can occur.
Protein Synthesis
Replication Problem
Given a DNA strand with the following nucleotide
sequence, what is the sequence of its complimentary
strand?
3- TACCACGTGGACTGAGGACTCCTCTTCAGA -5
Answer
Given a DNA strand with the following nucleotide
sequence, what is the sequence of its complimentary
strand?
3- TACCACGTGGACTGAGGACTCCTCTTCAGA -5
5- ATGGTGCACCTGACTCCTGAGGAGAAGTCT -3
ANSWER
Given a DNA strand with the following nucleotide
sequence, what is the sequence of its complimentary
mRNA strand?
3- TACCACGTGGACTGAGGACTCCTCTTCAGA -5
3- AUGGUGCACCUGACUCCUGAGGAGAAGUCU -5
Codon / Anticodon
Given a mRNa strand with the following
nucleotide sequence, what are the sequence
(anticodons) of its complimentary tRNA strands?
3- AUGGUGCACCUGACUCCUGAGGAGAAGUCU -5
Answer
Given a mRNA strand with the following nucleotide
sequence, what are the sequence (anticodons)
of its complimentary tRNA strands?
3- AUGGUGCACCUGACUCCUGAGGAGAAGUCU -5
Protein Translation
Given the following
sequence of mRNA,
what is the amino
acid sequence of the
resultant
polypeptide?
AUGGUGCACCUGA
CUCCUGAGGAGAA
GUCU
Met-val-his-leu-thr-pro-glu-glu-lys-ser