Documente Academic
Documente Profesional
Documente Cultură
Crown viruses
General CharacteristicsCoronaviruses
Coronaviridae
Arteriviridae
Flaviviridae
Togaviridae
footandmouthdiseasevirus
porcineenteroviruses
felinecalicivirus
coronaviruses
equinearterivirus,PRRSV
flaviviruses(WNV)
pestiviruses(BVD)
equineencephalitisviruses
Virus
Human coronavirus 299E
Transmissible gastroenteritis
Feline infectious peritonitis
Disease
Canine coronavirus
Common cold
Gastroenteritis
Peritonitis, pneumonia virus,
meningoencephalitis,
panophthalmitis, wasting
Enteritis
II (mammalian)
Common cold
Hepatitis, encephalomyelitis,
enteritis
Gastroenteritis
Vomiting, wasting, and
encephalomyelitis
III (avian)
Tracheobronchitis, nephritis
IV (avian)
Enteritis
Coronavirus Structure
Coronavirus replication. Numbers of mRNAs and locations of nonstructural (NS) proteins may vary for different cornaviruses. Virions
bind to the cell membrane and enter by membrane fusion or endocytosis. Viral genomic RNA acts as mRNA to direct the synthesis of viral
RNA-dependent RNA polymerase. This enzyme copies the viral genomic RNA to form full-length (-) strand templates. These templates are
copied to form new (+) strand genomic RNA, an overlapping series of subgenomic mRNAs, and leader RNA. All mRNAs are capped and
polyadenylated and form a nested set with common 3 ends. Each mRNA codes for a single polypeptide. The N protein binds to novel viral
RNA to form helical nucleocapsids. E1, E2, and E3 glycoproteins are produced on membrane-bound polysomes. Some coronaviruses do not
encode E3. Cornaviruses that encode E3 cause hemadsorption in infected cells. Virions are formed by budding at membranes of the Golgi
apparatus and the RER, but not at the plasma membrane. Virions are released by cell lysis or by fusion of post-Golgi, virion-containing
vesicles with the plasma membrane.
Replication
SARS
SARS Coronavirus
SARS
Where did SARS come from?
SARS diagnosis
Serology ELISA, questionable
Virology-PCR
Viruses of Veterinary
Importance
Coronaviruses
Rotavirus
Coronavirus
BVD
Bredavirus
Calicivirus
Parvovirus
Astrovirus
Susceptability of neonates
Rotaviruses 4 to 14 days
Coronavirus 4 to 30
4days
Colostral
Antibodiesingut
Susceptibleperiod
Diagnosis
FA of fecal samples
EM
Prevention
Vaccination of pregnant
animals
Colostrum for 2 weeks
vaccines against
calf diarrhoea
The incubation period is usually 1-3 days, and all litters within the
farrowing house are commonly affected at the same time.
The clinical signs in piglets are vomiting followed by a watery
diarrhea and rapid loss of weight. The diarrhea is profuse, with an
offensive odor, and often contains curds of undigested milk.
Piglets infected when under 7 days of age generally die within 2 to 7
days of the onset of signs; piglets over 3 weeks of age usually live
(may be unthrifty for several weeks). In growing, finishing, and adult
swine the disease is commonly associated with inappetence and
diarrhea of a few days' duration, and may even go unnoticed. Sows
infected late in pregnancy may develop pyrexia, but they are
otherwise normal and rarely abort.
sera.
FIP
new virus?
old virus, new disease
two serotypes
serotype I
FeCV, serotype 2
BothserotypescanleadtoFIPcausingstrains
FeCV
1/10,000 nucleotides
quasispecies
invariant portion of genome
FIPvirus
epidemiology
Exposure to FeCV
epidemiology (FIP)
sporadic
clustered9 (2-3 cats)
rarely epidemic - 40% mortality
persistent infections
immunohistochemistry
premunition
reason for rare horizontal transmission of FIP
FIP
pathogenesis
FEC
Milddiarrhoea
orrespiratoryillness
virus
immune system
persistentinfection
lowlevelofreplicationinepithelialandlymphoidcells
pregnancy in
young queens
weaning, sale,
shipment,
adaptation
elective surgery
concurrent infections
(FeLV, FIV ?)
Virus
immune
system
increasedvirusreplication>virulentmutants
increasedabilitytogrowinmacrophages
immunemediatedlysisofinfectedcells
cytokinesdrawinmoresusceptiblecells
vascularpermeability
immunecomplexrelateddamage
clinical signs
common signs
clinical signs
wet form
peritonitis
pleuritis
dry form
diagnosis
serology
prognosis?
diagnostic alogrithm
(Horzinek and Lutz)
diagnostic algorithm
diagnostic algorithm
control
vaccine
Primucell FIP
Intranasal ts virus
management
The primary target for viral replication is the trachea, but the virus
also replicates in the lungs, ovaries, and lymphoid tissue.
Avian infectious bronchitis. (A) One synonym for the disease is gasping disease. (B) Thick
mucopurulent exudate in the trachea. (C) Nephrosis. The kidney is pale and enlarged to about five
times normal size. (D) Embryos from embryonated hens eggs inoculated via the allantoic cavity
with serial dilutions of virus when 9 days old, and examined 11 days later. Amounts of virus
diminish in pairs from right to left in the top row, and from left to right in the bottom row.
Other coronaviruses of
importance in veterinary
medicine
Porcine respiratory coronavirus (PRCV)
Porcine Hemagglutinating
Encephalomyelitis virus
Porcine epidemic diarrhea virus (PEDV)
Canine coronavirus (CCV)
Turkey coronavirus (Bluecomb disease
of turkeys)
Mouse Hepatitis Virus