WWW.NCBI.NLM.NIH.

GOV
PubMed: Scientific Journals
Entrez: Keyword Search of Database
BLAST: Sequence Queries
OMIM: Online Mendelian Inheritance in Man
Books
TaxBrowser
Structure: 3D Molecular Structures

Sequence Files
Since the information relevant to biological processes is contained in the
gene or protein sequence, all genetic and protein data are contained in
sequence files.
Importantly, there is a directionality that exists in nature that is conserved
in the sequence file;
Nucleic Acids are always written 5 to 3 (describing the 5 or 3 free
hydroxyl group used in the phosphodiesterase bond).
nucleic acids (genes): 5-AGCTCGTGTAGACCATTC-3
Amino Acids are always written with the free amino (N-terminus) first and
the carboxylic acid (C-terminus) last.
amino acids (proteins): amino-IPKERYRGQIESIWA-carboxy

DNA is Double Stranded

Anti-parallel Configuration
Top strand is ALWAYS written 5 to 3
When DNA is written in file, top strand is represented and bottom strand is assumed.

5
3

3
5

5
3

3
5
AGTCGTGATCTGCTAAATGTCTCGAAGTTCGATGCTAG
||||||||||||||||||||||||||||||||||||||
TCAGCACTAGACGATTTACAGAGCTTCAAGATACGATC

Courier font is preferred for writing sequence data since letter spacing
is independent of character content.

FASTA
File
Format

>gi|1924939|emb|X98411.1|HSMYOSIE Homo sapiens partial mRNA for myosin-IF

CAGGAGAAGCTGACCAGCCGCAAGATGGACAGCCGCTGGGGCGGGCGCAGCGAGTCCATCAATGTGACCC
TCAACGTGGAGCAGGCAGCCTACACCCGTGATGCCCTGGCCAAGGGGCTCTATGCCCGCCTCTTCGACTT
CCTCGTGGAGGCCATCAACCGTGCTATGCAGAAACCCCAGGAAGAGTACAGCATCGGTGTGCTGGACATT
TACGGCTTCGAGATCTTCCAGAAAAATGGCTTCGAGCAGTTTTGCATCAACTTCGTCAATGAGAAGCTGC
AGCAAATCTTTATCGAACTTACCCTGAAGGCCGAGCAGGAGGAGTATGTGCAGGAAGGCATCCGCTGGAC
TCCAATCCAGTACTTCAACAACAAGGTCGTCTGTGACCTCATCGAAAACAAGCTGAGCCCCCCAGGCATC
ATGAGCGTCTTGGACGACGTGTGCGCCACCATGCACGCCACGGGCGGGGGAGCAGACCAGACACTGCTGC
AGAAGCTGCAGGCGGCTGTGGGGACCCACGAGCATTTCAACAGCTGGAGCGCCGGCTTCGTCATCCACCA
CTACGCTGGCAAGGTCTCCTACGACGTCAGCGGCTTCTGCGAGAGGAACCGAGACGTTCTCTTCTCCGAC
CTCATAGAGCTGATGCAGTCCAGTGACCAGGCCTTCCTCCGGATGCTCTTCCCCGAGAAGCTGGATGGAG
ACAAGAAGGGGCGCCCCAGCACCGCCGGCTCCAAGATCAAGAAACAAGCCAACGACCTGGTGGCCACACT
GATGAGGTGCACACCCCACTACATCCGCTGCATCAAACCCAACGAGACCAAGCACGCCCGAGACTGGGAG
GAGAACAGAGTCCAGCACCAGGTGGAATACCTGGGCCTGAAGGAAAACATCAGGGTGCGCAGAGCCGGCT
TCGCCTACCGCCGCCAGTTCGCCAAATTCCTGCAGAGGTATGCCATTCTGACCCCCGAGACGTGGCCGCG
GTGGCGTGGGGACGAACGCCAGGGCGTCCAGCACCTGCTTCGGGCGGTCAACATGGAGCCCGACCAGTAC
CAGATGGGGAGCACCAAGGTCTTTGTCAAGAACCCAGAGTCGCTTTTCCTCCTGGAGGAGGTGCGAGAGC
GAAAGTTCGATGGCTTTGCCCGAACCATCCAGAAGGCCTGGCGGCGCCACGTGGCTGTCCGGAAGTACGA
GGAGATGCGGGAGGAAGCTTCCAACATCCTGCTGAACAAGAAGGAGCGGAGGCGCAACAGCATCAATCGG
AACTTCGTCGGGGACTACCTGGGGCTGGAGGAGCGGCCCGAGCTGCGTCAGTTCCTGGGCAAGAAGGAGC
GGGTGGACTTCGCCGATTCGGTCACCAAGTACGACCGCCGCTTCAAGCCCATCAAGCGGGACTTGATCCT
GACGCCCAAGTGTGTGTATGTGATTGGGCGAGAGAAGATGAAGAAGGGACCTGAGAAAGGTCCAGTGTGT
GAAATCTTGAAGAAGAAATTGGACATCCAGGCTCTGCGGGGGGTCTCCCTCAGCACGCGACAGGACGACT
TCTTCATCCTCCAAGAGGATGCCGCCGACAGCTTCCTGGAGAGCGTCTTCAAGACCGAGTTTGTCAGCCT
TCTGTGCAAGCGCTTCGAGGAGGCGACGCGGAGGCCCCTGCCCCTCACCTTCAGCGACACACTACAGTTT
CGGGTGAAGAAGGAGGGCTGGGGCGGTGGCGGCACCCGCAGCGTCACCTTCTCCCGCGGCTTCGGCGACT
TGGCAGTGCTCAAGGTTGGCGGTCGGACCCTCACGGTCAGCGTGGGCGATGGGCTGCCCAAGAACTCCAA
GCCTACCGGAAAGGGATTGGCCAAGGGTAAACCTCGGAGGTCGTCCCAAGCCCCTACCCGGGCGGCCCCT
GGCGCCCCCCAAGGCATGGATCGAAATGGGGCCCCCCTCTGCCCACAGGGGGGGGCCCCCTGCCCCCTGG
AGAAATTCATTTGGCCCAGGGGGCACCCACAGGCCTCCCCGGCCCTCCGTCCACATCCCTGGGATGCCAG
CAGACGACCCCGGGCACGTCCGCCCTCAGAGCACAACACAGAATTCCTCAACGTGCCTGACCAGGGGATG
GCCGGCATGCAGAGGAAGCGCAGCGTGGGGCAACGGCCAGTGCCTGTGGGCCGACCCAAGCCCCAGCCTC
GGACACATGGTCCCAGGTGCCGGGCCCTATACCAGTACGTGGGCCAAGATGTGGACGAGCTGAGCTTCAA
CGTGAACGAGGTCATTGAGATCCTCATGGAAGATCCCTCGGGCTGGTGGAAGGGCCGGCTTCACGGCCAG
GAGGGCCTTTTCCCAGGAAACTACGTGGAGAAGATCTGAGCTGGGCCCTGGGATACTGCCTTCTCTTTCG
CCCGCCTATCTGCCTGCCGGCCTGGTGGGGAGCCAGGCCCTGCCAATGAAAGCCTCGTTTACCTGGGCTG
CAATAGCCTAAAAGTCCAATCCTTTGGCCTCCAGTCCTTGCCCAGGCCCTGGGTCACCAGGTCACTGGTG
CAGCCCCCGCCCCTGGGCCCTGGTTTTCCTCCAACATCACACCTGCTGCCCATTGTCCAAAACTGTGTGT
GTCAAAGGGGACTAACAGCAGAATTTACCTCCCAACTGCCATGTGATTAAGAAATGGGTCTTGAGTCCTG
TGCTGTTGGCAAAGTTCCAGGCACAGTTGGGGAGGGGGGGCCGGAATCCGC

A sequence in FASTA format begins with a single-line description, followed by

lines of sequence data.
1) The description line starts with a greater than symbol (">").
2) The word following the greater than symbol (">") immediately is the "ID"
(name) of the sequence, the rest of the line is the description. The "ID" and
the description are optional.
3) All lines of text should be shorter than 80 characters.
4) The sequence ends if there is another greater than symbol (">") symbol at
the beginning of a line and another sequence begins.

The following example contains two protein sequences (Example1, Example2):

>Example1 envelope protein

ELRLRYCAPAGFALLKCNDADYDGFKTNCSNVSVVHCTNLMNTTVTTGLLLNGSYSENRT
QIWQKHRTSNDSALILLNKHYNLTVTCKRPGNKTVLPVTIMAGLVFHSQKYNLRLRQAWC
HFPSNWKGAWKEVKEEIVNLPKERYRGTNDPKRIFFQRQWGDPETANLWFNCHGEFFYCK
MDWFLNYLNNLTVDADHNECKNTSGTKSGNKRAPGPCVQRTYVACHIRSVIIWLETISKK
TYAPPREGHLECTSTVTGMTVELNYIPKNRTNVTLSPQIESIWAAELDRYKLVEITPIGF
APTEVRRYTGGHERQKRVPFVXXXXXXXXXXXXXXXXXXXXXXVQSQHLLAGILQQQKNL
LAAVEAQQQMLKLTIWGVK
>Example2 synthetic peptide
HITREPLKHIPKERYRGTNDTLSPQIESIWAAELDRYKLVKTNCSNVS

Sequences are expected to be represented in the standard IUB/IUPAC amino acid and nucleic acid
codes, with these exceptions:
1)
2)
3)
4)

Lower-case letters are accepted and are mapped into upper-case

A single hyphen or dash can be used to represent a gap of indeterminate length
In amino acid (protein) sequences, U and * are acceptable letters.
N for unknown nucleic acid residue; or X for unknown amino acid residue.

5) mRNA is often listed as cDNA, and the U is replaced with T

The nucleic acid codes supported are:

A adenosine
M A C (amino)
C cytidine
S G C (strong)
G guanine
W A T (weak)
T thymidine
BGTC
U uridine
D G AT
R G A (purine)
H AC T
Y T C (pyrimidine)
VGCA
K G T (keto)
N A G C T (any)
- gap of indeterminate length

For those programs that use amino acid (protein) query sequences
(e.g. BLASTP and TBLASTN), the accepted amino acid codes are:
A alanine
B aspartate
C cystine
D aspartate
E glutamate
F phenylalanine
G glycine
H histidine
I isoleucine
K lysine
L leucine
M methionine
N asparagine

P proline
Q glutamine
R arginine
S serine
T threonine
U selenocysteine
V valine
W tryptophan
Y tyrosine
Z glutamine
X any
* translation stop
- gap of indeterminate length

FASTA
File
Format

>gi|1924939|emb|X98411.1|HSMYOSIE Homo sapiens partial mRNA for myosin-IF

CAGGAGAAGCTGACCAGCCGCAAGATGGACAGCCGCTGGGGCGGGCGCAGCGAGTCCATCAATGTGACCC
TCAACGTGGAGCAGGCAGCCTACACCCGTGATGCCCTGGCCAAGGGGCTCTATGCCCGCCTCTTCGACTT
CCTCGTGGAGGCCATCAACCGTGCTATGCAGAAACCCCAGGAAGAGTACAGCATCGGTGTGCTGGACATT
TACGGCTTCGAGATCTTCCAGAAAAATGGCTTCGAGCAGTTTTGCATCAACTTCGTCAATGAGAAGCTGC
AGCAAATCTTTATCGAACTTACCCTGAAGGCCGAGCAGGAGGAGTATGTGCAGGAAGGCATCCGCTGGAC
TCCAATCCAGTACTTCAACAACAAGGTCGTCTGTGACCTCATCGAAAACAAGCTGAGCCCCCCAGGCATC
ATGAGCGTCTTGGACGACGTGTGCGCCACCATGCACGCCACGGGCGGGGGAGCAGACCAGACACTGCTGC
AGAAGCTGCAGGCGGCTGTGGGGACCCACGAGCATTTCAACAGCTGGAGCGCCGGCTTCGTCATCCACCA
CTACGCTGGCAAGGTCTCCTACGACGTCAGCGGCTTCTGCGAGAGGAACCGAGACGTTCTCTTCTCCGAC
CTCATAGAGCTGATGCAGTCCAGTGACCAGGCCTTCCTCCGGATGCTCTTCCCCGAGAAGCTGGATGGAG
ACAAGAAGGGGCGCCCCAGCACCGCCGGCTCCAAGATCAAGAAACAAGCCAACGACCTGGTGGCCACACT
GATGAGGTGCACACCCCACTACATCCGCTGCATCAAACCCAACGAGACCAAGCACGCCCGAGACTGGGAG
GAGAACAGAGTCCAGCACCAGGTGGAATACCTGGGCCTGAAGGAAAACATCAGGGTGCGCAGAGCCGGCT
TCGCCTACCGCCGCCAGTTCGCCAAATTCCTGCAGAGGTATGCCATTCTGACCCCCGAGACGTGGCCGCG
GTGGCGTGGGGACGAACGCCAGGGCGTCCAGCACCTGCTTCGGGCGGTCAACATGGAGCCCGACCAGTAC
CAGATGGGGAGCACCAAGGTCTTTGTCAAGAACCCAGAGTCGCTTTTCCTCCTGGAGGAGGTGCGAGAGC
GAAAGTTCGATGGCTTTGCCCGAACCATCCAGAAGGCCTGGCGGCGCCACGTGGCTGTCCGGAAGTACGA
GGAGATGCGGGAGGAAGCTTCCAACATCCTGCTGAACAAGAAGGAGCGGAGGCGCAACAGCATCAATCGG
AACTTCGTCGGGGACTACCTGGGGCTGGAGGAGCGGCCCGAGCTGCGTCAGTTCCTGGGCAAGAAGGAGC
GGGTGGACTTCGCCGATTCGGTCACCAAGTACGACCGCCGCTTCAAGCCCATCAAGCGGGACTTGATCCT
GACGCCCAAGTGTGTGTATGTGATTGGGCGAGAGAAGATGAAGAAGGGACCTGAGAAAGGTCCAGTGTGT
GAAATCTTGAAGAAGAAATTGGACATCCAGGCTCTGCGGGGGGTCTCCCTCAGCACGCGACAGGACGACT
TCTTCATCCTCCAAGAGGATGCCGCCGACAGCTTCCTGGAGAGCGTCTTCAAGACCGAGTTTGTCAGCCT
TCTGTGCAAGCGCTTCGAGGAGGCGACGCGGAGGCCCCTGCCCCTCACCTTCAGCGACACACTACAGTTT
CGGGTGAAGAAGGAGGGCTGGGGCGGTGGCGGCACCCGCAGCGTCACCTTCTCCCGCGGCTTCGGCGACT
TGGCAGTGCTCAAGGTTGGCGGTCGGACCCTCACGGTCAGCGTGGGCGATGGGCTGCCCAAGAACTCCAA
GCCTACCGGAAAGGGATTGGCCAAGGGTAAACCTCGGAGGTCGTCCCAAGCCCCTACCCGGGCGGCCCCT
GGCGCCCCCCAAGGCATGGATCGAAATGGGGCCCCCCTCTGCCCACAGGGGGGGGCCCCCTGCCCCCTGG
AGAAATTCATTTGGCCCAGGGGGCACCCACAGGCCTCCCCGGCCCTCCGTCCACATCCCTGGGATGCCAG
CAGACGACCCCGGGCACGTCCGCCCTCAGAGCACAACACAGAATTCCTCAACGTGCCTGACCAGGGGATG
GCCGGCATGCAGAGGAAGCGCAGCGTGGGGCAACGGCCAGTGCCTGTGGGCCGACCCAAGCCCCAGCCTC
GGACACATGGTCCCAGGTGCCGGGCCCTATACCAGTACGTGGGCCAAGATGTGGACGAGCTGAGCTTCAA
CGTGAACGAGGTCATTGAGATCCTCATGGAAGATCCCTCGGGCTGGTGGAAGGGCCGGCTTCACGGCCAG
GAGGGCCTTTTCCCAGGAAACTACGTGGAGAAGATCTGAGCTGGGCCCTGGGATACTGCCTTCTCTTTCG
CCCGCCTATCTGCCTGCCGGCCTGGTGGGGAGCCAGGCCCTGCCAATGAAAGCCTCGTTTACCTGGGCTG
CAATAGCCTAAAAGTCCAATCCTTTGGCCTCCAGTCCTTGCCCAGGCCCTGGGTCACCAGGTCACTGGTG
CAGCCCCCGCCCCTGGGCCCTGGTTTTCCTCCAACATCACACCTGCTGCCCATTGTCCAAAACTGTGTGT
GTCAAAGGGGACTAACAGCAGAATTTACCTCCCAACTGCCATGTGATTAAGAAATGGGTCTTGAGTCCTG
TGCTGTTGGCAAAGTTCCAGGCACAGTTGGGGAGGGGGGGCCGGAATCCGC

FASTA
File
Format

TinySeq
XML

>gi|1924940|emb|CAA67058.1| myosin-IF [Homo sapiens]

QEKLTSRKMDSRWGGRSESINVTLNVEQAAYTRDALAKGLYARLFDFLVEAINRAMQKPQEEYSIGVLDI
YGFEIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIRWTPIQYFNNKVVCDLIENKLSPPGI
MSVLDDVCATMHATGGGADQTLLQKLQAAVGTHEHFNSWSAGFVIHHYAGKVSYDVSGFCERNRDVLFSD
LIELMQSSDQAFLRMLFPEKLDGDKKGRPSTAGSKIKKQANDLVATLMRCTPHYIRCIKPNETKHARDWE
ENRVQHQVEYLGLKENIRVRRAGFAYRRQFAKFLQRYAILTPETWPRWRGDERQGVQHLLRAVNMEPDQY
QMGSTKVFVKNPESLFLLEEVRERKFDGFARTIQKAWRRHVAVRKYEEMREEASNILLNKKERRRNSINR
NFVGDYLGLEERPELRQFLGKKERVDFADSVTKYDRRFKPIKRDLILTPKCVYVIGREKMKKGPEKGPVC
EILKKKLDIQALRGVSLSTRQDDFFILQEDAADSFLESVFKTEFVSLLCKRFEEATRRPLPLTFSDTLQF
RVKKEGWGGGGTRSVTFSRGFGDLAVLKVGGRTLTVSVGDGLPKNSKPTGKGLAKGKPRRSSQAPTRAAP
GAPQGMDRNGAPLCPQGGAPCPLEKFIWPRGHPQASPALRPHPWDASRRPRARPPSEHNTEFLNVPDQGM
AGMQRKRSVGQRPVPVGRPKPQPRTHGPRCRALYQYVGQDVDELSFNVNEVIEILMEDPSGWWKGRLHGQ
EGLFPGNYVEKI

<?xml version="1.0"?>
<!DOCTYPE TSeq PUBLIC "-//NCBI//NCBI TSeq/EN" "http://www.ncbi.nlm.nih.gov/dtd/NCBI_TSeq.dtd">
<TSeq>
<TSeq_seqtype value="nucleotide"/>
<TSeq_gi>1924939</TSeq_gi>
<TSeq_accver>X98411.1</TSeq_accver>
<TSeq_taxid>9606</TSeq_taxid>
<TSeq_orgname>Homo sapiens</TSeq_orgname>
<TSeq_defline>Homo sapiens partial mRNA for myosin-IF</TSeq_defline>
<TSeq_length>2711</TSeq_length>
<TSeq_sequence>CAGGAGAAGCTGACCAGCCGCAAGATGGACAGCCGCTGGGGCGGGCGCAGCGAGTCCATCAATGT
</TSeq>

FASTA File Format(note: U = T)

>gi|1234|my name from genetic code in DNA
ATGATTTGTCACGCTGAGCTC-AAAGCT
AACGAGTAA

>gi|1234|my name translated into protein

MICHAEL-KANE*
A alanine
B aspartate
C cystine
D aspartate
E glutamate
F phenylalanine
G glycine
H histidine
I isoleucine
K lysine
L leucine
M methionine
N asparagine

P proline
Q glutamine
R arginine
S serine
T threonine
U selenocysteine
V valine
W tryptophan
Y tyrosine
Z glutamine
X any
* translation stop
- gap of indeterminate
length

Where do we get DNA sequence information?

DNA Sequencing Methods
-conversion of biological/bioanalytical data into sequence information
There are automated, high-throughput sequencing centers that COMPLETELY
automate (robotics and information systems) DNA sequencing, preliminary
identification and publishing.

DNA Sequencing (old method)

5-AAACCAGGCCGATAAGGTACTACACGAAAAAAA-3
TTTTTTT
dATP
dCTP
dTTP
dGTP

ddATP32
ddCTP32
ddTTP32
ddGTP32

Step 1. Extend complementary

sequence using free nucleotides
with limiting amounts of radioactive
terminating nucleotides.
Step 2. Run product out on a
electrophoresis gel.
Step 3. Place gel against
radiographic film, develop.

A G C T

AAACCAGGCCGATAAGGTACTACACGAAAAA
|||||||||||||||||||||||||||||||||||||||
TTTGGTCCGGCTATTCCATGATGTGCTTTTTTT
TTGGTCCGGCTATTCCATGATGTGCTTTTTTT
TGGTCCGGCTATTCCATGATGTGCTTTTTTT
GGTCCGGCTATTCCATGATGTGCTTTTTTT
GTCCGGCTATTCCATGATGTGCTTTTTTT
TCCGGCTATTCCATGATGTGCTTTTTTT
CCGGCTATTCCATGATGTGCTTTTTTT
CGGCTATTCCATGATGTGCTTTTTTT
GGCTATTCCATGATGTGCTTTTTTT
GCTATTCCATGATGTGCTTTTTTT
CTATTCCATGATGTGCTTTTTTT
TATTCCATGATGTGCTTTTTTT
ATTCCATGATGTGCTTTTTTT

DNA Sequencing new method)

http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/D/DNAsequencing.html

GGATCCTGCAAGGAGGGATACAAATTACATACATTTGTCAAAACCCACAGCATGTTGACCACCAGGAGGAG
ACCCCATGTGACTCCAGGACCCTGGTTGATAACAACGTATCGAGATTCCTCACATGGAACCAGTGCGCTC
CTGTGGTGGAGGGTGTACCTGTGTCAGGGCAGGGGGTACGTGGACATTTTCTGCAGTTTTTGATCAATTT
TGCAATGAACTAAATCTGTGGTATAAAAATAAAGTCTATTAAAAGAATCCAAGGCTCCCTCTCATCTCACGAT
AAGATAAAGTCCCCATCCATTTTACTCCTCTCAGCCCTGGAGAAAGGAGAGGCCAGGTCCCACCACCTTC
CACCAGCATGGACCCCCAGTCCAGACCCCACGCCTTTTCTCAGCATCCTCAGACCAGCAGGACTTGCAG
CAATGGGGAATTAGGCACCTGACTTCTCCTTCATCTACCTTTGGCTGGGGGCCTCCAGCCTTGACCTTCG
CTCTGAGAGTCTCAGGCAGGTCCAGAGCCAGTTCTCCCATGACGTGATATGTTTCCAGAGCAGGTTCCTG
GGTGAGATAAAAGGATTTGGGCTGAACAGGGTGGAGGGAGCATTGGAATGGCACTCAGGGCAAAGGCAG
AGGTGTGCGTGGCAGCGCCCTGGCTGTCCCTGCAAAGGGCACGGGCACTGGGCACTAGAGCCGCTCGG
GCCCCTAGGACGGTGCTGCCGTTTGAAGCCATGCCCCAGCATCCAGGCAACAGGTGGCTGAGGCTGCT
GCAGATCTGGAGGGAGCAGGGTTATGAGCACCTGCACCTGGAGATGCACCAGACCTTCCAGGAGCTGGG
GCCCATTTTCAGGTAAAGCCCTCCCTGGCCCTCGCTGGGAACACCCAGATCCCTGCCCCTGCTGCCCAG
GACCCTGCCAGGCACTCAGCACTGCCATTCCCAGCAGGTCCCGGCACTCTGCATCCTTTGGAGGATGGG
GAAGGAGTGCAGCACATGCTGGTCTGTGGTGCTGCCAGGGCAGGGGATAGTGCAGAGAAAACCCCAGC
TCACTGCAGAGAGGGCAGGACTCAGAAGCACTAAAGTTGAAAGGTTCCAGGGAGCCAGCAGGAGGGCTT
TAGCTGTGAAGCCGCTAATCCAGGAGCAGGGAGGGTGGACAGGAGACACTTTGGATTGGGACTGCAGGG
TGGGGCCACGAGGGACATGACCCCGTCCAGCAGGGCCTCCTGCTTGGCCCCACAGGTACAACTTGGGA
GGACCACGCATGGTGTGTGTGATGCTGCCGGAGGATGTGGAGAAGCTGCAACAGGTGGACAGCCTGCAT
CCCTGCAGGATGATCCTGGAGCCCTGGGTGGCCTACAGACAACATCGTGGGCACAAATGTGGCGTGTTC
TTGTTGTAAGCGGCGAGTTGGGAGCTGAGAGCTGGGAGCAGGGTGGGCAGCCTGGGTGTAGGGGGGA
GGCGAGAGAGGTAGGACCCAAAAGCACATCTGCCCTGGGCCCCTGTGGTGGGCAGTGAGGGTGAGCAC
CCGGCCCAGAGGACGGCCATCCTGTGGGGTCGCGTCTGCACTGTGGGTTGGGGAAGCAGGGCGGTGG
TGGAGAAATGGGCACGGGCACCTCTGCAGAGAAGACGCAGAGCAATGAGCCCTTCTGTGTAGTGAGAAC
CCGCTCTGCACCAACCTCGGCGGCTGCTTTCTCTTGCGGTCTGGGGACTGTCCTTCCCATAGGTCAGAA
AACTGAGGCCCTGAGAAGGGGACTTCCACTGGCCCAGGTCACAGGCTGAGTGCTGAGCCTGGTGTTCG
CCGGGGCCGCAGCCTCCCTCAGGGCGCTCAGGGTCCCTGCAGTCCTGGCAAACCTTCCTGATGGGGAC
AGTCCGGGGCAGGAGGCAGGTGGGGACGCAGGTGGCTGGTGGTTCCGTTGTTCTCAGAAGCAAGGCAC
AAGGTGGGGCGGTTGATGGCACTGGGGAGGATGTTTCCTGGCCCGTGGAGAGGGTGGCGCCTGGTCAG
GTGGGCAGGGAGAGGCTGATGCTTGGAGTCGGTCACCTGCAGGGATGTTGTCATTAGGACGGGGGAAG
GACTGGATGAGGATGTCACAGTGGTGACAGCCCCCACTCCATGGTAGGAAGGGAACGCTATTGGGAATAG
TGGGGTTTAGGTAAAAGGGCACCCGTGGGTCGGGGCCTTCACTGAGGCTGGCCTATAGATGACATCTGG
GAGAGAGTCAGGACCCAGGAAGGCAGGTCCAGGA