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MARKERS AND
THEIR
APPLICATIONS
Definition
PROTEIN AFLP
Low polymorphism
Requires expression of trait / RAPD
gene
Dominance effect
Expression sex limited Depend upon sequence of DNA
Expressed late in life
They are protein produced by expression of gene
DNA markers
Non-PCR Based,
RFLP- Restriction fragment length polymorphism.
PCR Based
RAPD- Random amplification of polymorphic DNA.
AFLP-Amplified fragment length polymorphism.
SCAR-Sequence characterize amplified region.
STS- Sequence tagged sites.
EST-Express sequence tags.
SNP-Single nucleotide polymorphism.
SSR-Simple sequence repeats
CAPS-Cleaved amplified polymorphic sequences.
Properties of Ideal Genetic Marker
it must be polymorphic.
Co-dominant inheritance.
A marker should be evenly and frequently
distributed throughout the genome.
It should be easy, fast and cheap to
detect.
It should be reproducible.
High exchange of data between
laboratories.
DEFINITION:- RAPD that is defined by differences
between individuals in terms of DNA
regions either being or not being amplified in a
polymerase chain reaction primed by random
oligonucleotides sequences.
2. An excess of selected
decaoligonucleotide
added.
Definition
Any of the visualization techniques will give the same results. The
visualization techniques used will depend on the laboratory condition
Principle
Advantages
Disadvantages
Disadvantages
-it is inherited as dominant traits
-there is a bands due to relatively short primer
-the production of non-parental bands in the offspring of
known pedigree warrants its use with extreme care
-it is sensitive to change in PCR conditions
YO U
AN K
T H