ENTEROBACTERIACEAE

Aerobic bacterial flora in the large intestine of

man
Non sporing, non acid fast, gram negative
bacilli
Grouped family Enterobacteriaceae
Capsulated / non capsulated
Motile peritrichate flagella/ non motile
Reduce nitrate to nitrites
Form catalase not oxidase
Based on lactose fermentation LF / NLF
Enterobacteriaceae includes the following genus
Escherichia coli
Klebsiella
Proteus
Salmonella
Shigella
Characters E.coli Klebsiella Proteus Salmonella Shigella
Morpholog G-ve rods G-ve rods G-ve G-ve rods G-ve rods
y pleomorphi
c rods
motile, Non motile Motile Motile Non motile
(peritricha capsulated Non spore Non spore Non spore
te) former former former
Non spore Non Non non
former capsulated capsulated capsulated
Capsulate Pili present Pili present
d (some)

Cultural
Characteri
stics
Aerobic/fa Aerobic/fac Aerobic/fac Aerobic/fac Aerobic/fac
O2 req c anaerobic anaerobic anaerobic anaerobic
anaerobic 6.8 - 7.2 7 - 7.2 68 7.4
PH 6.5 - 7.2
12C - 25-40C 15 41C 10 40C
Temperatur 10 43C
e 40C/Opt
37C
Characters E.coli Klebsiella Proteus Salmonella Shigella
Nutrient Large,thick, Large Greyish Large, Small,
agar Moist dome white circular low circular,
smooth, shaped swarming convex Convex,
opaque mucoid colonies smooth smooth
greyish colonies with fishy translucent translucent
white or seminal colonies colonies
colonies odour

Mac Deep pink Pale colour Pale colour

conkey Pink colonies(LF Pale/colorle (NLF) (NLF)
agar colonies ) ss
(LF) (NLF) Non Hemolytic
Blood agar Hemolytic Hemolytic
Hemolytic Non
Hemolytic Tetrathiona
Selective tebroth -
media EMB- - DCA Pale
Green - colour
metallic Wilson Blair
sheen Bismuth
sulphate
agar-
Inkjet black
Selenite F
Characters E.coli Klebsiella Proteus Salmonella Shigella
Biochemica
ls
++-
tests ++-- --++ P.vulgaris
-+-+ -+--
IMViC -+-
Gelatin ve Sugar fertn P.mirabilis Catalase
H2S ve +ve H2S ve +ve
Urease +ve H2S ve
Urease -ve Urease ve
LF-ve
Urease +ve

Virulence Surface ag K&O O ohne O-somatic O-ag

factors (O L K) antigen hauch& ag Fimbrial ag
Fimbriae Enterotoxin H hauch H Exotoxin
Toxins similar to O-somatic flagellar ag Enterotoxin
Hemolysin ST toxin of H Vi- Vero/cytoto
Enterotoxin E.coli flagellar Virulence xin
ST & LT Cross ag
verotoxin reactivity X
strain(non
motile)OX2
/OX19/OXK
with
Characters E.coli Klebsiella Proteus Salmonella Shigella

Pathogene UTI, UTI, UTI, Gastroenteri Bacillary

sis Gastroenter Broncho- Abscess tis dysentry
itis pneumoni Summer Septicemia, Entry oral
Pyogenic, a, diarrhoea Enteric route
infectious Diarrhoea, , fever, Infectn dose-
Septicemia. Septicemi Otitis, Infectn dose- 10-100
a. Septicem 106 bacilli bacilli
ia Inc period 7- Inc period
14 days 1- 7 days

Treatment Neomycin, Cefuroxim Sensitive Vaccine Oral

Doxycycline e, to TAB rehydration
, Cefotoxim gentamy Ampicillin Nalidixic
Norfloxacin, e, cin, Amoxycillin acid/
Trimethopri Trimethopr Kanamyc cotrimoxazol norfloxacin
me ime, in, e
clotrimoxic Ciproflax
lav cin,
cotrimox
azole