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PRAMUDJI HASTUTI
BIOCHEMISTRY DEPARTMENT
FACULTY OF MEDICINE
1
Is it different when :
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Acid : litmus paper red
Base : litmus paper blue
ARRHENIUS THEORY :
+
Acid : substances in the water yield H ion
_
Base : substances in the water yield OH ion
BRNSTED-LOWRY THEORY:
+
Acid : substances yield proton (H ) / donor
proton
H+ + :O:H H:O:H
H H
H+ + : N:H H:N:H H+ + NH3 NH4+
H H
coordination 4
_
HCl H + Cl
+
H2SO4 H+ +
_
HSO4
_ _
HCN H + CN
+
HSO4 H+ +
=
SO4
_ +
CH3COOH H + CH3COO NH4 H+ +
+
_
NH3
_
H2CO3 H + HCO3
+
NH3 H+ +
_
NH2
5
_ _
Weakest acid strongest conjugate base
_
(HCN) CN combines firmly with proton
_
3) CH3COOH + NH3 NH4 + CH3COO +
+
1) most H + H2O H3O very strong acid
+
electronegative Cl >>> H
attract electrons >>
proton release >>
lower pK pH <<
Diprotic
Carbonic acid 6.37 , 10.25
Succinic acid 4.21 , 5.64
Glutaric acid 4.34 , 5.41
Triprotic acid
_
Acid : H > OH
+
_
Base : OH > H+
_
pH of 1 N H = 0
+
pH of 1 N OH = 14
pH scale : 0 - 14
_
Except gastric juice : concentration H & OH very small
+
_ = _
H2PO4 H+ + HPO 4 K2 = 2 X 10 7
= _3 _
HPO H + PO
14
+
K = 3.6 X 10 12
EFFECT SALT IN DISSOCIATION ACID & BASE,
BUFFER SOLUTION
_ _
CH3COONa CH3COO + Na+ CH3COOH= CH3COO
_
NH4Cl NH4 + Cl
+
NH4Cl = H4 +
_
NH4OH NH 4
+
+ OH NH4OH>>NH4 +
_
NH4Cl increase OH pH
[proton acceptor]
pH = pKa + log
[proton donor]
+ 1 ml 0,1 N HCl
( HCl convert 1 ml 0.1 M salt acid)
+ 1 ml 0,1 N HCl
( HCl convert 4 ml 0.025 M salt acid)
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c) 16 ml 0,1 M CH3COONa
4 ml 0,1 N CH3COOH
pH = 4.73 + log 16/4 = 5.33
+ 1 ml 0,1 N HCl
( HCl will convert 1 ml 0.1 M salt acid)
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Maximum efficiency :
- ratio salt & acid = same
IMPORTANT of BUFFER
- regulating pH fluids, tissues
- Laboratory : control pH
- media culture,
- reactions that catalyzed by
enzymes
22
BUFFERS OF BLOOD PLASMA :
- phosphate/biphosphate
- protein/proteinate
23
BUFFERS OF RED BLOOD CELLS :
- Protein Hb >>>(pH = 7.25)
(carbonate/bicarbonate <<)
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