Poultry Science Association

Latin American Scientific Conference

Connecting the Global Poultry Science Community

L-Arginine for broilers and the

influence on blood lipids levels
Srgio Turra Sobrane Filho*, Paulo Borges Rodrigues, Eduardo Machado
Costa Lima, Mrvio Lobo Teixeira de Abreu, Sebastin Dorado
Montenegro
University of Lavras, Lavras, Minas Gerais, Brazil

Presenter by Srgio Turra

Sobrane Filho
Introduction:

Abdominal fat: The problem of Poultry Industry

Breeding Advances and Nutritional Advances

Choct et al. (2000) found that modern broiler strains

contain 15% to 20% fat and >85% of this fat is not
physiologically required for body function

Affects consumer acceptance

Fouad et al. (2014)

Objective :

To evaluate the effects of

different levels of L-arginine
supplementation on the blood
lipids content of broilers in the
period from 29 to 42 days old
Material and Methods :
780 broilers (males and females)-> Cobb 500

29 days old-> males ( 1403 22 g) and females

(1294
Guo et al. (2011)-> 5th weekbroiler
50 g)chickens increase the
diameter of adipocytes
Guo et al., (2011)-> 5 semana os frangos de corte aumentam o dimetros dos
adipcitos
Experimental design: Distribute in Randomized Blocks

Blocks -> boxes and cage

2 (sex) X 5 (levels of L-arginine suplemmentation)

Levels L-arginine
factorial schedule
suplementation:
0.3, 0.6, 0.9, 1.2 %
3 replications of 23 birds per boxes and 3 birds
per cages
 Ingredient (g Basal Diet Calculated composition
kg-1) (%)
Metabolizable energy 3150
Corn 59.80 (kcal/Kg)
Soybean meal 30.23 Crude Protein (%) 18.75
45%PB
Digestible Lisine (%) 1.045
Soy oil 4.83
Digestible Methionine 0.760
Limestone 0.80
Digestible Arginine 1.162
Dicalcium 1.18
Digestible Leucine 0.724
phosphate
Digestible Isoleucine 0,724
Salt 0.46
Digestible Valine 0,812
L-lisine HCL 0.20
Digestible Histidin 0.425
DL-Methionine 0.25
Digestible teronine 0.681
L-Treonina 0.05
Digestible triptofano 0.205
L-Valina 0.02
Choline Chloride 0.04
60% Basal Diet formulation was based
on foods composition and
Trace mineral- 0.09 nutritional requirements proposed
vitamin premix by Rostagno et al. (2011)
Anticoccidial 0.05
Kaolin 2.00
Material and Methods :
At 42 days old: 6 birds per treatment -> 12 h fasting->
blood was collected (5 ml)
Variable response:
Total cholesterol Triglycerides HDL cholesterol
LDL cholesterol
VLDL cholesterol

Sinnowa SX-3000M semi-automatic biochemical

analyzer and Labtest serological kits

Data was submitted to ANOVA and polynomial

regression with 5% of probability
Results and discussion:
CV=8,37

CV=10,95

CV=12,32

CV=15,2
CV=15,2

Triglycerides Total Cholesterol cHDL cLDL VLDLc

Females Males

There was not interaction between sex and L-

arginine in all variables responses (P> 0.05), neither
isolated sex effect (P> 0.05)
Results and discussion:
52.53 Optimal level
=0.66% 51.04

48.95

45.38 45.44
Dow(0,66%) ->Fouad et al. (2013)- Decrease Expression Fatty
acid synthesis (FAS) gene
Up (0,66%)-> L-arginine-> glutamate-> -cetoglutarato (citric
acid cycle)->oxaloacetate
triglyceride levels increased when increasing arginine
0% catabolism0.3%
rate, with a consequently
0.6% higher
0.9%fatty acids 1.2%
synthesis.
Triglycerides (mg dL-)
Quadratic effect (P= 0.0138)
Triglycerides= 17,45x2 - 23,10x + 53.10
R = 92.59%
Results and discussion:
136.68

128.16
124.81

119.94
118.78

Fouad et al (2013)- L-arginine (oxide nitric reduction

expression HMG-CoA reductase (synthesis cholesterol).

0% 0.3% 0.6% 0.9% 1.2%

Total cholesterol (mg dL-)

Linear effect (P= 0.0008)
Total cholesterol = -14,67x+134,47
R = 92.94%
Results and discussion:

93.53
91.91
90.85 91.07

86.63

0% 0.3% 0.6% 0.9% 1.2%

cholesterol HDL (mg dL-)

Results and discussion: Optimal level
32.64
=0.66%
10.5
29.09
26.45 10.2
20
9.78
17.5 cholesterol LDL cholesterol VLDL
(mg dL-) 9.07 9.08 (mg dL-)

0% 0.3% 0.6% 0.9% 1.2% 0% 0.3% 0.6% 0.9% 1.2%

Linear effect (P= 0.0106) Quadratic effect (P= 0.0133)

-12,23x+32,48 3,50x2-4,64x+10,62
R = 92,64% R = 85.16%
[LDLc] = (TC HDLc) - (TG/5) [VLDLc] = TG/5
Conclusion:

Supplementation of 0.66% l-
arginine shows the best
blood lipids relation,
possibly reducing the fat
content in broilers.
 Poultry Science Association
Latin American Scientific Conference
Connecting the Global Poultry Science Community

L-Arginine for broilers and the

influence on blood lipids levels

Thank you for attention!

Questions ?
Suggestions ?
Presenter by Msc. Srgio Turra
Sobrane Filho
sergio.sobranefilho@gmail.com