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General
Concepts
Signs
Symptoms
Etiology
Epidemiology
Pathogenicity
Virulence
Photos used from various UF/IFAS Extension Publications or provided by UF/IFAS faculty and staff, unless otherwise stated.
Symptom of Disease
observation of how the host
is manifesting infection by a
pathogen and disease
development due to a
University of Florida - IFAS 2
Signs vs.
Symptoms
Disease
growth)
Powdery mildew
Signs of
Fungal
mycelia
Disease mycelia
Mosaic
Ringspot
University of Florida - IFAS 16
Plant Pathology
Terms
Pathogenicity: the pathogen either
does
or does not cause a disease; a
question
with yes or no answer
Virulence: relative capacity of
pathogen to
cause disease; range from minimal
damage
to dead plant
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Etiology and Pathogenicity
Testing
1) Consistent isolation of a pathogen
from symptomatic host tissue
2) Pathogen is grown in pure culture and
its characteristics documented
3) Inoculation of a healthy plant with the
pure culture of the pathogen, and
inoculated plant must then develop
symptoms similar to those observed
initially
4) Recovery of the same pathogen used
for inoculation purposes
This is general scheme; non-culturable plant pathogens
have special rules.
University of Florida - IFAS 18
Etiology and Pathogenicity
Testing
Example: Fusarium Wilt of
Queen Palm
3) F. proliferatum (9 isolates)
known pathogen of palms; can cause wilt
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Control F. oxysporum
F. incarnatum-equiseti F. proliferatum
University of Florida - IFAS 21
Plant Pathology
Terms
Epidemiology: study of the factors
influencing the initiation,
development and spread of
infectious disease
But, how do pathogens
enter the plant?
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How do pathogens enter
Viruses and plant?
Viroids and Fastidious
Bacteria
most require vectors; a few
mechanical entry
Bacteria most enter through
natural
openings or wounds
Fungi & Oomycetes enter through
natural
openings, wounds; by mechanical
University of Florida - IFAS 23
How do pathogens enter
plant?
Plants have natural
openings: stoma or
stomata (plural)
Plant
Epidermis
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How do pathogens enter
Leafhopper
plant?
Bacteria
(insect vector) on water
Use mouth film, enter
parts to through
penetrate stoma
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How do pathogens enter
Leafhopper
plant?
Bacteria
(insect vector) on water Fungal Spore
Use mouth film, enter entering
parts to through through stoma
penetrate stoma
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How do pathogens enter
Leafhopper
plant?
Bacteria
(insect vector) on water
Appressorium
fungal structure Fungal Spore
Use mouth film, enter from spore for entering
parts to through direct through stoma
penetrate stoma penetration
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How do pathogens enter
Leafhopper
plant?
Bacteria
(insect vector) on water
Appressorium
fungal structure Fungal Spore
Use mouth film, enter from spore for entering
parts to through direct directly
penetrate stoma penetration through stoma
Fungal Hyphae
can grow between
cells or penetrate
cells
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Disease
Development
pathogen comes in contact with
plant
pathogen infects plant penetration,
can be
direct or indirect; with or without
vector
incubation period time between
penetration
and first appearance of symptoms
pathogen increases within plant,
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Disease
Development
Environmental conditions influence
each and every step in disease
development process!!
Susceptible
Host
Favorable DISEASE
Environment
Pathogen
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Disease
Development
Susceptible
Nematod Host Fungus
e W. Deacon, Univ. of
Edinburgh
Favorable Pathogen
Environment
Mite Insect
USDA/AR
S University of Florida - IFAS 32
How do plant
pathogens move from
plant to plant if they
are not moved by a
Wind dispersal of spores
vector?
Splash dispersal of spores rain,
irrigation
Physical movement of soil-borne
pathogens that dont produce spores
ex: soil tillage
Nematodes swim or move with soil
Seed associated internal or
external
University of Florida - IFAS 33
How do plant
pathogens move from
plant to plant if they
are not moved by a
vector?
Time to have some fun!
Cheap, easy way to
demonstrate
spore dispersal without
water or spores!
University of Florida - IFAS 34
Splash Dispersal of
Spores