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Water
[Water] > [Water]
[Salt] < [Salt]
Osmotic Pressure < Osmotic Pressure
Osmosis is the movement of water from a high concentration to a low concentration. In
this illustration, two compartments (A and B) are separated by a semipermeable
membrane (broken vertical line). The water concentration in compartment A is greater
than the concentration in compartment B because of the presence of salt (X) in B.
Therefore, water will move down its concentration gradient from A to B. The force
needed to prevent this water movement is called osmotic pressure.
Tonicity
Hemopoietic process
Stage one: Hemopoietic stem cells
self renewal, steady numbers, active differentiation.
Stage two: committed progenitors
directional differentiation (CFU-GEMM, CFU-E, CFU-
GM, CFU-MK, CFU-TB). [CFU: colony- forming unit
Stage three: precursors
morphologic occurrence of various original blood
cells.
Hemopoietic stem cells
Basic characteristics
Self renewal in high degree, constant from young to old
age.
Multi- directional differentiation
Large potential proliferation, Hemopoietic stem cells
produce about 11011 blood cells releasing to blood for
use.
Surface sign
According to CFU (colony forming unit), using
fluorescence-activated cell sorting (FACS), its main
surface sign is CD34+CD38-Lin-and CD34-CD38-Lin-.
Note
CD: cluster of differentiation of antigen on the white
blood cells;
Lin: systemic specific antigen on the hemopoietic cells.
2.Hemopoietic microenvironment
Hemopoietic microenvironment:
It includes stromal cell secreting extracellular matrix (ECM),
multihemopoietic regulating factor, hemopoietic nerves and
blood vessels.
Stromal cells in the marrow come from fibrocyte, reticulocyte,
endothelial cell, ectoblast cell, monocyte, engulfing cell,
osteoblast and osteoclast.
Stromal cells supply two material: one is soluble hemopoietic
growth factor, another is membrane-combined adhesive
molecule.
Extracellular stroma synthesized and secreted by marrow
stromal cell filling cellular interstice contains big molecules,
such as collagen (typeI, II, III, IV), glycoprotein (fibronectin,
laminin, hemopoieticnectin ) and protein amylose (sulfate
cartilagetin, sulfate heparin, hyaluronic acid and sulfate
dermatin, etc).
Hemopoietic cells must adhere to stromal cell and is in the
hemopoietic microenvironment for survival.
Hemopoietic process
Hemopoietic process
Hemopoietic process
3.Erythrocyte Physiology
Shape and number of red blood cells (RBC)
Shape of RBC: like biconcave disc
Diapedisis
material (metabolic production,
antigen-antibody complex,
bacteria, toxin, etc).
Phagocytosis: It is a process that
WBCs enclose and engulf exotic Blood
or extraneous material, and use Vessel
intracellular enzyme digesting
them.
Metamorphose
Physiological Characteristics
and Functions of WBC
Neutrophil
Another name, polymorphonuclear, PMN, 6~8 h in the
vessels, diapedisis, chemotaxis and phagocytosis
(using its hydrolyzed enzyme)
Function: It plays a very important role in nonspecific
cellular immunity system which is against pathogenic
microorganism, such as bacteria, virus, parasite, etc.
Clinic relation:
Number of neutrophil greatly increase occurring in
acute inflammation and earlier time of chronic
inflammation.
number decrease of neutrophil will result in poor
resistibility and easily suffering from infection.
Physiological Characteristics
and Functions of WBC
Eosinophil
Circadian changes: Its number is lower in the morning
and higher at night.
Function:
1. It limits and modulates the effects of basophil on fast
allergic reaction.
2. It is involved in immune reaction against worm with
opsonization.
Clinic relation: Its number increase when person suffers
from parasite infection or allergic reaction.
Physiological Characteristics
and Functions of WBC
Basophil
Circulatory time: 12 hours
Basogranules contain heparin, histamine, chemotactic
factors and chronic reactive material for allergic reaction.
Function: It is also involved in allergic reaction.
1. Heparin serves as lipase cobase and speeds up fatty
decomposition.
2. Histamine and chronic reactive material increase
permeability of capillary and contract bronchia smooth
muscle, and result in allergic reaction such as measles,
asthma.
3. Eosinophil chemotactic factor A released by basophil
can attract eosinophil collection and modify eosinophil
function.
Physiological Characteristics
and Functions of WBC
Monocyte
Its body is large, diameter about 15~30 m without granule
Function:
1. It contains many nonspecific lipase and displays the
powerful phagocytosis.
2. As soon as monocytes get into tissue from blood , it change
name called macrophage activating monocyte- macrophage
system to release many cytokins, such as colony stimulating
factor (CSF), IL-1, IL-3, IL-6, TNF, INF-, ,etc.
3. Cytokins induced by monocyte may modulate other cells
growth.
4. Monocyte- macrophage system plays a very important role in
specific immune responsive induction and regulation.
Physiological Characteristics
and Functions of WBC
Lymphocyte
Classification: It can be separated into T- Lymphocyte
and
B- Lymphocyte.
Function:
1. Lymphocytes serve as a nuclear role in immune
responsive reaction.
2. T- Lymphocytes involved in cellular immunity.
3. B- Lymphocytes involved in humoral immunity.
Clinic relation: Numbers increase of lymphocytes occur
in
Leukopoiesis, Regulation and Breakage
a
PL: phospholipid Ca2+
a
CL: cross linking fibrin PL
a
HK: high molecular weight kininogen
a
S: Subendothelium
Ca2+
PK: prekallikrein a CLa
K: kallikrein
Mechanism of Blood Coagulation
Anticoagulative system in blood
Cellular anticoagulative system: Liver cell and reticular endothelial cell
could engulf blood clotting factor, tissue factor, prothrombin complex
and soluble fibrin monomer.
Humoral anticoagulative system:
1. Amino acid protease inhibitors in blood include antithrombin III, Cl-
inhibitor, 1 antitrypsin, 2 antiplasmin, 2 huge globin, heparin
coenzyme II, protease nexin-1 (PN-1) to combine with FIXa, FXa,
FXIa, FXIIa and thrombin and then inactivate them for anticoagulation.
Heparin can intensify functions of antithrombin III.
2. Protein C system are protein C (PC), thrombomodulin (TM), protein
S and Protein C inhibitors. Main functions of PC consist in It
inactivates FVa, FVIIIa with phospholipid and Ca2+; It blocks FXa
combining with platelet phospholipid membrane to reduce
prothrombin activation; It stimulates plasminogen activators release
to trigger fibrinolysis; Protein S is a coenzyme of PC and greatly
intensify functions of PC.
3. Tissue factor pathway inhibitor (TFPI) mainly coming from vessel
endothelial cells inhibits FXa and inactivates FVIIa-TF complex to
block extrinsic pathway of coagulation with negative feed back.
4. Heparin used in the clinic widely is due to It combines with
antithrombin III to increase functions of antithrombin III; It
stimulates vessel endothelial cell greatlu releasing TFPI and other
anticoagulative material; It intensifies PC activation and stimulates
vessel endothelial cell releasing plasminogen activators to increase
fibrinolysis. [lower molecular weight heparin is less hemorrhage]
4.Fibrinolysis
Fibrinolytic system is involved in fibrinolysis, tissue
repair and vessel rebirth.
Two fibrinolytic systems: cellular one and plasma
one. The former is leucocyte, macrophage,
endothelial cell, mesothelial cell and platelet to
engulf and digest fibrin. The latter is plasminogen
activators (PA) and its inhibitors (PAI),
plasminogen, plasmin.
Basic steps:
Kallikrein (Intrinsic pathway)
Endothelial cells (Extrinsicpathway )
(Urokinase, uPA) Cl-inhibitors
tPA uPA uPAG
PAI-1
Plasminogen Plasmin
2-antiplasmin
2-huge globin Fibrin
Fibrin or fibrinogen dissolution
Blood Coagulation and Fibrinolysis
Antifibrinolysis:
Fibrinolytic Inhibitors and Its Functions
Main fibrinolytic inhibitors: They are plasminogen
activator inhibitor type-1 (PAI-1, in platelet), 2-
antiplasmin (in liver), 2-huge globin, 1-antitrypsin,
antithrombin III, alexin C1 inhibitor.
PAI-1 synthesis and release: PAI-1 made by endothelial
cell, smooth muscular cell, mesothelial cell,
megakaryocyte is stored in platelet with inactive form.
Some factors such as thrombin, IL-1, TNF, etc
stimulate its release from platelet.
PAI-1 function: It inhibits tPA (tissue-type plasminogen
activator) limiting local fibrinolysis of thrombus.
2-antiplasmin characteristics: (1) Quick effect, (2)
Inhibit plasminogen adhering to fibrin; (3) Combine
with fibrin chain and block fibrinolysis
Clinic relation: Innate deficiency of 2-antiplasmin often
brings about serious hemorrhage.
V. Blood Group
History: ABO blood group system was firstly found by
Landsteiner in 1901.
Definition for blood group*: Types of specific antigens
on the blood cell.
Agglutination: Combination of the same antigen (or
named agglutinogen, glycoprotein/glycolipid on the
membrane of blood cell) and antibody (or named
agglutinin, r-globin in serum) results in harmful immune
reactions showing hemolysis.
Human leukocyte antigen, HLA have widespread
distribution in the body and involves in immune
repulsive reaction of organ transplant.
Platelet antigens such as PI, Zw, Ko, etc may bring
about fever heat when transfusion occur.
1. RBC Agglutination
A A Anti-B
B B Anti-A
AB A+B
O Anti-A+Anti-B
2. ABO blood group system
A A1 A+ A1 Anti-B
A2 A Anti-B+ Anti-A1
B B Anti-A
AB A1B A+ A1 +B
A2B A+B Anti-A1
O Anti-A+Anti-B
ABH Antigen chemical structure in ABO
blood group system
Antigen of blood group
Ushering material
O(H)-antigen
A-antigen
B-Antigen
N-acetamide N-acetamide
Galactose Glucose galactose
Sugar Glucose
Inheritance of ABO blood group
Inheritance: The A, B, H agglutinogen in ABO
blood group system controlled by gene which
is located at allele on No.9 chromosome
(9q34.1-q34.2).
Genotype and Phenotype:
Genotype and Phenotype in ABO blood group system
Genotype phenotype
OO O
AA, AO A
BB, BO B
AB AB
Inheritance of ABO blood group
Genetic relationship of ABO blood group
Parents Offspring possible Offspring impossible
blood group blood group blood group
OO O A, B, AB
AA O, A B, AB
AO O, A B, AB
BB O, B A, AB
BO O, B A, AB
BA O, A, B, AB ____
ABO A,B O, AB
ABA A , B, AB O
ABB A , B, AB O
ABAB A , B, AB O
Distribution of ABO blood group
Donator Receiver
Serum Serum
Main side of
Subordinary side
Decision
agglutination of agglutination
- - Perfect
match, transfusion
+ +, - No
match, transfusion
- + Transfusion
under emergency
+: Agglutination; -: No agglutination
Types of Transfusion
According to source of transfusion,
allogenetic transfusion (more use),
autologous transfusion.
According to component of transfusion, whole
blood transfusion, transfusion of blood
components
Autologous transfusion has some advantages:
It decreases infection.
It blocks syndrome (fever, hemolysis) induced by
allogenetic transfusion.
It stimulates bone marrow hemopoiesis towards
RBC.
Transfusion of blood components is good.