STABILITY EVALUATION AND ANTIOXIDANT ACTIVITY OF A

COSMETIC WITH Lactobacillus acidophilus INTACT CELLS

BENDER, S.1; FARIA, L.O.2 ; VASCONCELOS,H.L.; 2COSTA, A.C.3; TONDING, F.3; CARLETTO, A. 3; SANTOS, C.A.S.3
1 Master degree student.2Associate Professor- Pharmaceutical Sciences Post-graduation Program; 3Undergraduate Pharmacy
Course - PIC/UNIOESTE. Western Paran State University, Cascavel, Paran, Brazil. suzanabender@hotmail.com

INTRODUCTION RESULTS & DISCUSSION

Lactobacillus acidophilus is the kind of lactic acid bacteria better known and
studied, but its topical action is little explored [1]. Recent patents have
suggested the development of cosmetics with its use as a 'bioactive
ingredients' [2]. The objective of this study was to evaluate the stability and
antioxidant activity of a cosmetic formulation using the freeze-dried whole
cell of Lactobacillus acidophilus for the purpose of protecting the skin
against oxidative action over time.
MATERIAL & METHODS
The methodology used in this study is demonstrated in the figures:
Centrifugation: After second cycle the samples of all concentrations were liquid.
The Natrosol gel has typical behavior of non-newtonian pseudoplastic fluid
possibly, as the viscosity decreases with increasing shear stress
(centrifugation).It also thixotropic behavior, that is the potential that has the
structure to rearrange when the substance is left standing for a long period of
time.
Evaluation of Heat Stress:
40C - change the appearance to opaque; Change the color from
transparent to white; Change the pH-4.5 to 3,8-acidified product.
80C- glue to the tube and dry-increased permeability of the
membrane. It was possible to consider that the intact cell was
dissolved and developed in the gel at this temperature.

Figure 7. Change of cosmetic gel in 40C Figure 8. Change of cosmetic gel in 80C

Evaluation of frost/defrost cycle:

From the sixth day there were changes in

Figure 1. Preparation of the Cosmetic Gel [3]
viscosity. The product became less fluid at
room temperature in all concentrations. In
frost/defrost cycle, the same changes were
observed from the fourth day. From the
Figure 2. Preliminary stability- Centrifugation [4]
ninth day at both temperatures the gel
changed its transparent and color to white.

Evaluation of Antioxidant Activity by the DPPH:

40.0
40.0 Room Temperature Frost/defrost cycle

35.0
35.0

30.0
30.0

25.0
25.0
%AA

%AA

0.5% 20.0 0.5%

20.0
1.0% 1.0%
1.5% 1.5%
2.0% 2.0%
15.0
15.0

10.0
10.0

5.0
5.0

Figure 3. Preliminary stability- Heat Stress [4] Figure 4. Preliminary stability- frost/defrost cycle [5]
0.0 0.0
T0 T4 T8 T12 T0 T4 T8 T12

Time
Time

Figure 9. %AA at Room Temperature Figure 10. %AA Frost/Defrost cycle

The antioxidant activity was 29.80% Antioxidant activity was 29.80%

(T0) to 34.21% (T12), at room (T0) to 36.58% (T4) in the highest
temperature. concentration. This activity was not
sustained over time and decrease
36.58% (T4) up to 24.34% (T12).

CONCLUSION
Figure 5. Gel prepare for evaluation I- (T0) and II- (T4,T8,T12)-DPPH [6]
Lactobacillus acidophilus was developed on the gel changing their
characteristics analyzed at different temperatures. This development may
also have led to an increase in antioxidant activity.

Thanks to Araucria Foundation and CAPES for the financial support.

REFERENCES
1.COSTA-VALE, J. Desenvolvimento de um Creme Antioxidante Para Uso Dermatolgico a partir de um Princpio
Ativo Contendo Pr/Probiticos. 2015. Trabalho de concluso de curso (Bacharel em Farmcia)-Graduao em
Farmcia, Faculdade Assis Gurgacz, Cascavel, 2015.
2 TERAN, C. Probiotic Technology. Active Concepts. 2011.
3. BRASIL. Agncia Nacional de Vigilncia Sanitria (ANVISA). Formulrio Nacional da Farmacopeia Brasileira. 2
ed. Braslia, 2011.
4 IDSON, B. Stability testing of emulsions, I. Drug & Cosmetics Industry. v. 151, n. 1, p. 27-30,1993a.
5 BRASIL. Agncia Nacional de Vigilncia Sanitria (ANVISA). Guia de Controle de Qualidade de Produtos
Cosmticos: Uma abordagem sobre os ensaios fsicos e qumicos. Braslia, 2007. 130 p.
6 FREIS, A. T.; FRASSON, A. P. Z. Avaliao Da Atividade Antioxidante De Cosmticos Anti-idade. REVISTA
CONTEXTO & SADE IJU EDITORA UNIJU v. 0 n. 19 JUL./DEZ. 2010 p. 17-23.
Figure 6. Evaluation of Antioxidant Activity by the DPPH [6]