Chemostat in Series

UNIVERSITY OF SANTO TOMAS
FACULTY OF ENGINEERING
CHEMOSTA
T
I N
SERIES
CHEMOSTAT:
INTRODUCTION:
- a bioreactor where in the nutrients are
supplied at a constant flow rate and the
cell density is adjusted with the
supplied essential nutrients for growth.
2
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INTRODUCTION:
SlideDoc Slidedocs help you spread your smart thinking by
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written copy. Their scannable nature makes them great pre-
read, reference, and leave-behind materials. Their
modularity makes it easy for people to incorporate your
ideas into their own communications. And these features
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written documents and presentations.
Text from duarte.com/slidedocs
3
This -software,
Fromthatchemical environment
is intended is static.
to be read and referenced instead
template is -of projected.
The system is equipped with a gas flow
for a meter. Agitation and aeration provided
suitable mass transfer.
INTRODUCTION:
-combining
The liquid level is controlled
visual communications with shortwith
chunksanof
outlet
written pump.
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Fresh medium is pumped into the culture
vessel.
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into their own liquid level And
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reservoir.
4
This
IN SERIES:
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CHEMOSTAT limiting nutrients may be
varied in each stage,
resulting in different cell
physiology and cellular
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systems.
5
MULTI STAGE SYSTEM
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metabolites And these
use as features
medicines, flavorings, and recreational
drugs.
Culture of genetically engineered cells

Genetic Engineering
6
DIAGRAM
MULTI STAGE SYSTEM

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P10
GROWTH PRODUCT
S TA G E F O R M AT I O N S TA G E
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Can be used when parameters (X,P) cannot
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15
Step 1. Get EXAMPLE
kinetics data (X In a two-stage chemostat system, the first
vsThis
t & P vs t) in tank is 600 l and the second
read andone is 900instead
l.
GRAPHICAL METHOD
software, that is intended to be referenced
batch culture
template is The flowrate is 100l/h, determine the
of projected.
concentrations of cells and product in each
for a of the tank.
16
XP L E
Step 2. Get dX/dt E X A M t P t
vs X from the 0
In a two-stage 0 0
chemostat system, the first0
This data and
kinetics 1.00tank is 6003.00l and the second 0 one l. 3
is 900instead
GRAPHICAL METHOD
software, that is intended to be read and referenced
plot
template is 2.40The flowrate
of projected. 6.00 is 100l/h, determine
0 the 6
for a 4.00concentrations
8.00 of cells and product in each
0.025 8
of the tank.
SlideDoc 5.10 9.00 0.050
Slidedocs help you spread your smart thinking by
9
6.50
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communications with
7.10
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and leave-behind materials. Their 11
modularity makes
7.50 11.0it easy for people to incorporate your 12
0.180
7.70
together make 12.0
slidedocs the perfect0.260
companion to both 13
7.75 13.0 0.355 14
8.00 15.0 0.475 15
8.00 16.0 0.545 16
0.590 17
0.600 18 17
Step 3.1. On the EXAMPLE
graph of dX/dt~X, y D1( X1 X 0)
draw a line of A slidedoc is a visual document, developed in presentation
This
GRAPHICAL METHOD
y=D 1 (X1 -X0) F referenced instead
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=F/V1, X0=0 inis r (X S )
of projected.
( X1 X 0)
the feed, X1 is
x ,n x, n V1
for a
determined from Dn ( X n X n 1) 100 l/h
dX/dt (g/L-h)
SlideDoc
the intersection. thinking( Xby1 0)
Slidedocs help you spread your smart
combining visual communications with 600 l chunks of
short
X 0.167
written copy. Their scannable nature
1
makesXthem great pre-
1
read, reference, and leave-behind
67 materials. Their
modularity makes it easy .1for people to incorporate your
y=0
X (g/L) x1 is 7.1g/l
18
graph of X,P~t, P1
is determined by A slidedoc is a visual document, developed in presentation
This
GRAPHICAL METHOD
the X1 at the software, that is intended to be read and referenced instead
template
respect time is of projected.
for a X1=7.1 g/l
Text from duarte.com/slidedocs P1=0.09 g/l
19
Step 4. Determine
Step 4.1. Plot the EXAMPLE
graph
X of dP/dt~P
2 and P2 in the
from the kinetics
second stage: A slidedoc is a visual document, developed in presentation
This
data of P, draw a
GRAPHICAL METHOD
line of y=D (P -
template
P ), D =F/V , Pis
2 2 y D 2 ( P2 P1 )
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1 2 2 2
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is determined
F
dP/dt (g/L-h)
from the
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intersection. ( P P ) thinking by
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2
. 09)
100l / h ( P -0
1 1 2

modularity makes it easy for
y= 0 ( P 0.09g / l )
.1people to incorporate your
900l 2
0.111 ( P 0.09g / l )
2
P1=0.09 g/l P (g/L) P2=0.53 g/l
20
graph of X,P~t, X2
is determined by A slidedoc is a visual document, developed in presentation
This
GRAPHICAL METHOD
the P2 at the software, that is intended to be read and referenced instead
template
respect time.is of projected.
for a X2=7.95
g/l
SlideDoc
X (g/L)
Slidedocs help
X you spread
=7.1 g/l your smart thinking by
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P =0.53 g/l
read, reference, and leave-behind materials. Their 2
P1=0.09 g/l
Time
(hr)
21
A two stage chemostat system is used for
This the production of Pseudomonas sp. (1st reactor)
and formation of secondary metabolite (2nd
template is reactor). Pseudomonas sp. has a mass doubling
for a time of 2.4 hrs when grown on acetate. The
saturation constant using this substrate is 1.3 g/L
Sample problem 1:
SlideDoc (which is usually high), and cell yield on acetate
is 0.46 g cell/ g acetate. If the feed stream to a
chemostat contains 38 g/L acetate and the
volume for the 2 reactors are the same,
determine:
a. Maximum dilution rate
b. The product concentration of the
effluent of the second reactor when the
dilution rate is one-half of the
maximum. (qp= 0.02 g P/ g cell hr ; YP/S=
0.6 g P/ g S)
22
- In a two stage chemostat system,
This the volumes of the first and second reactors
are 450 L and 200 L respectively. The first
template is reactor is used for biomass production and
for a the second is for a secondary metabolite
formation. The feed flow rate to the first
SlideDoc reactor is F = 175 L/h, and the glucose
concentration is 6.0 g/L. Use the following
constants for the cells.
mm = 0.5 h-1, Ks = 0.25 g/L Y X/S = 0.45 g/g
Determine the cell and glucose concentrations

after the first stage.
Assume that growth is negligible in the second
stage and the specific rate of product
quiz
formation is qP = 0.01 gP/g cell hr, and Y P/S

= 0.58 gP/gS. Analyze and estimate the23

Chemostat in Series

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UNIVERSITY OF SANTO TOMAS

Culture of genetically engineered cells

MULTI STAGE SYSTEM

P1=0.09 g/l P (g/L) P2=0.53 g/l

mm = 0.5 h-1, Ks = 0.25 g/L Y X/S = 0.45 g/g

Determine the cell and glucose concentrations

formation is qP = 0.01 gP/g cell hr, and Y P/S

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