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Bleeding Time and Clotting

Time Determination

 To:
1.Define hemostasis and describe how bleeding stops
from a finger prick.
2.Determine BT and CT by the routine laboratory
methods, and give their normal values.
3.Indicate clinical importance of performing BT and CT.
4.Name the conditions in which BT and CT are

 Vasoconstriction.
 Formation of platelet plug.
 Formation of a blood clot.
 Fibrinolysis.
Process of Haemostasis…
Clinical Importance of BT and CT

 History of spontaneous bleeding.

 Before minor and major surgeries.
 Before taking biopsy, especially from
bone marrow, liver, kidney, etc.
 Before and during anticoagulant
 Family history of bleeding disorder.
Bleeding Time (BT)

 Is the time interval between the skin

puncture and spontaneous, unassisted
(i.e., without pressure) stoppage of
 The BT test is an in vitro test of
platelet function.

 Filter paper.
 Stop watch.
 Lancet.
 Alcohol swabs.
Procedure of BT…

 Prick the finger.

 Touch site of prick with filter paper.
 Repeat procedure after 30 seconds.
 Circle the spots of blood.
 Note bleeding time.
 Write down your results.
It is usually significantly prolonged in the case of:

a) Thrombocytopenic purpura; more common.

It is due to low platelet count.
b) Thrombocytopathic purpura; less common
It is due to defect in platelet function.
Thrombocytopenic purpura
Clotting Time (CT)

 Is the time interval between the entry

of blood into the glass capillary tube
and formation of a fibrin thread.

 Capillary tube( non-heparenized).

 Stop watch.
 Lancet.
 Alcohol swabs.
Procedure of CT…
 Prick your finger.
 Touch one end of capillary tube with the
blood drop.
 Fill the capillary tube.
 Break small piece of capillary.
 Repeat procedure after every 30 seconds.
 When fibrin thread appears, stop the stop
Causes of prolonged clotting time:

a) Vitamin K deficiency:
It leads to failure of formation of some clotting factors by the liver
b) Haemophilia:
Group of hereditary genetic disorders that impair the body's ability
to control blood clotting, caused by deficiency of some clotting
c) Liver disease: due to defective formation of most of the clotting
Difference between BT & CT

 BT  tests platelet plug formation.

 CT  tests formation of clot.
 In hemophilia :
- BT normal.
- CT prolonged.
Anticoagulants & Antiplatelet
Agents for Clinical use

 Heparin.
 Warfarin.
 Aspirin.