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REAGENT

STRIPS
REAGENT STRIPS
• Second phase of urinalysis makes use of
Reagent Strip pads impregnated with
chemical reagents for analyte detection.
• Parameters Of The Reagent Strip Pad:
Four:ph,glucose,protein,specific Gravity
Ten:ph,protein,glucose,ketones,blood,
bilirubin,urobilinogen,nitrite,leukocytes,
and Specific Gravity
11th Parameter:ascorbic Acid
2 MAJOR TYPES OF REAGENT STRIPS:
Multistix(Siemens Healthcare Diagnostics, Deerfield, In)
Chemstrip(Roche Diagnostics, Indianapolis,IN)
• Some strips incorporate iodate, which eliminates vitamin C
interference via oxidation
• Parameters affected by ascorbic acid:
(blood,bilirubin,leukocyte esterase,nitrite,glucose)
• Reactions are indicated by specific color changes after strip
pads have been dipped into a well-mixed urine specimen within
3 seconds.
• Procedure for urinalysis manual should be reviewed yearly by a
designated authority
• Automated reagent strip reader (Principle: Reflectance
spectrometry)
REAGENT STRIP TEST
PROCEDURE
1. Mix the urine specimen well.
2. Let refrigerated specimens warm to room temperature before
testing.
3. Dip the reagent strip completely but briefly(3 seconds).
4. Remove excess urine by withdrawing the strip against the rim
of the container and by blotting the edge of the strip.
5. Compare reaction colors with the manufacturer’s chart under
a good light source at the specified time.
6. Perform backup tests when indicated.
7. Be alert for the presence of interfering substances.
8. Understand the principles and significance of the test;
read package inserts.
9. Relate chemical findings to each other and to the
physical and microscopic urinalysis results.
CARE OF THE REAGENT STRIPS
1. Store with desiccant in an opaque,tightly closed container.
2. Store below 30 degree celcius. Do not freeze.
3. Do not expose to volatile fumes.
4. Do not use past the expiration date.
5. Do not use if chemical pads become discolored.
6. Remove strips immediately prior to use.
QUALITY CONTROL OF THE
REAGENT STRIPS
1. Test open bottles of reagent strips with known positive and
negative controls every 24 hours.
2. Resolve control results that are out of range by further testing.
3. Test reagents used in backup tests with positive and negative
controls.
4. Perform positive and negative controls on new reagents and
newly opened bottles of reagent strips.
5. Record all control results and reagent lot numbers.
SOURCES OF ERROR
1. Improper technique (e.g unmixed specimen)
2. Allowing the strip to remain in the urine for an extended
period causing leaching of reagents from the pads.
3. Excess urine in the strip causes a run-over between chemicals
on adjacent pads producing distortion of colors. (protein-pH
pad mixture)
4. Not specific reaction time to read the results.
5. Not testing sample at room temperature(enzymatic reactions
on the strip are temperature-dependent.
REAGENT STRIP READING TIME

• 30 seconds:glucose,bilirubin
• 40 seconds: ketones
• 45 seconds: specific gravity
• 60 seconds: pH,protein,blood,urobilinogen,nitrite
• 120 seconds: leukocyte esterase
REFERENCES

- Urinalysis And Body Fluids 5th Edition By Susan King Strasinger & Marjorie Schaub Di Lorenzo
- www.googleimages.com

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