Documente Academic
Documente Profesional
Documente Cultură
1. COPI
2. COPII Know the start places and destinations
3. Clathrin
4. Mechanism of the assembly of COPII coated vesicle
Sar1-GDP and Sar1-GTP
Cop I:
Inner coat: Sec27
Outer coat: Ret1
GTPase: Arf1
GEF:Gea1/Gea2
Clatherin:
Heavy chain
Light chain
Adopter protein: AP2
Dispose waste
Or deliver chemicals to
other cells.
Alberts 14-53
The mitochondria
contains all enzymes
used in the TCA cycle
and oxidative
phosphorylation
Alberts 14-10
The electron transport chain is a set of three proton
pumps that use the energy of NADH to move
protons out of the matrix
Alberts 14-14
Question # 1:
The electron transport chain exports protons out of
the mitochondrial matrix. Therefore:
Alberts 14-13
Special Protein Translocators Transfer Single Polypeptide
Chains Across and Into Mitochondrial Membrane or Matrix
Alberts 12-23
Mitochondrial Import can be Reconstituted in vitro
Mitochondrial Import can be Reconstituted in vitro
40 kDa ->
20 kDa ->
5 kDa ->
The TOM and TIM23 Complexes Act In Concert
To Transfer Proteins Into The Matrix
Alberts 12-25
The Matrix Targeting Signal (+ charged) moves across
the inner membrane by electrophoresis due to the
Proton Motive Force
Alberts 12-26
Chaperone Pushing and Pulling, and the Proton Motive Force
Contribute To Different steps in Protein Translocation
Alberts 12-26
Mitochondrial HSP70 is required for the
translocation of the peptide into the Matrix
Once in the Matrix the proteins are folded by mHSP-70 and by mHSP-60
Alberts 12-26
Special Protein Translocators Transfer Single Polypeptide
Chains Across and Into Mitochondrial Membrane or Matrix
Alberts 12-23
Special Chaperones and the SAM complex insert Porins into
the outer mitochondrial membrane
The Intermembrane space has its own set of chaperones (not related to HSPs)
Proteins (like Porin) that go through this pathway have a targeting signal different from
MTS but still recognized by the TOM complex.
Alberts 12-27
Multi-pass transmembrane proteins are sorted
to the Inner Mitochondrial Membrane by the
TOM and TIM22 Complexes
Alberts 12-28
The OXA complex is used to “export” proteins out of the Matrix
OXA can transport proteins that were synthesized within the Matrix OR proteins that
were imported to the Matrix via TOM/TIM23 using an MTS. A separate special signal is
recognized by OXA.
Alberts 12-28
Question # 2:
You are going to measure the import of a protein into
mitochondria in the in vitro assay. You add a drug that
inhibits the electron transport chain. Which of the
following will be a likely effect of adding this drug?