Biology

Paper 3
Marking Scheme
1(a) Able to state two different observations correctly based on
these aspects :
P1 : Temperature of the water 3
P2 : The time taken when the mixtures fail to turn blue-black
.
Sample answer:
1. When the temperature of water is 370C, the time taken when the
mixtures fail to turn blue-black is 2 minutes.
2. No change of the colour of iodine in the tile at 50C and 600C.

Able to state two observation less accurately 2

Able to state observation at idea level. 1
No response or incorrect in response 0
1(b) Able to make two inferences correctly based on any two
aspects :
P1 :Activity of amylase is the highest/ lowest 3
P2: Activity of amylase is very slow at 50C
.P3: Activity of amylase is stop at 50C and 600C.
P4: The enzyme is denatured
Sample answer:
1. The time taken when the mixtures fail to turn blue-black is shortest
because the activity of amylase is the highest at 370C.
2. No change of the colour of iodine in the tile at 50C and 600C because
the activity of amylase is slow at 50C and stop at 600C
Able to make two inferences accurately based on any one aspect 2
Able to make inferences at idea level. 1
No response or incorrect in response 0
1(c)(d) Able to record all readings correctly.

Sample answer: 3

. Temperature/ 0C Time 1/ minute

5 36 minutes 0.028
28 5 minutes 0.200
37 2 minutes 0.500
45 6 minutes 0.170
60 36 minutes 0.028

Able to record 3 – 4 correct answer 2

Able to record 2 correct answer 1
1(e) Able to state two different observations correctly based on
these aspects :
P1 (MV): Temperature of the water 3
P2 (RV): The time taken when the mixtures fail to turn blue-black
.
The rate of activity of amylase
H: Relationship

Sample answer
The rate of the activity of amylase on starch increases with the
increases in temperature until it reaches the optimum temperature
of 370C
Able to make a hypothesis correctly based on any two aspects 2
Able to make a hypothesis at idea level 1
No response or incorrect in response 0
1(f) Able to state all the three variables and the methods to
handle the variables correctly
Sample answer: 3
Variables Method to handle
MV: Conducting the enzymes-catalyzed
Temperature of water
reactions at different
. temperature such as 50C, 280C, 370C,
450C, 600C
RV:
The time taken when the mixtures
Record the time taken when the
mixture fail to turn blue-black when
fail to turn blue-black
tested with iodine solution.

CV:
pH of water bath, Concentration of
Fixing the concentration of
amylase and the starch
the amylase and the starch

Able to get 3 – 5 ticks 2

Able to get 1 – 2 ticks 1
No response or incorrect in response 0
1(g) (i)Able to draw a graph of rate of the rate of enzyme
reaction against temperature:
Axes (A) : Both axes with units and uniform scales 3
Point (P) : All points are correctly plotted
Shape
. (S) : Correct shape of graph
Sample answer

Any 2 aspects correct 2

1 aspect correct 1
No response or incorrect in response 0
1(g)(ii) Able to state the relationship between temperature of
the water and the rate of enzymatic reaction
R : Any relationship statement 3
P1: Activity of enzyme increases until 370C
.P2: Activity of enzyme decreases when temperature higher than 370C
P3 :Activity of enzyme stop at 600C
Sample answer
The rate of the enzyme-catalyzed reaction increases when the
temperature increases until the optimum temperature of 370C. At
temperature higher than 370C, the rate of reaction decreases and stop
at 600C
Able to make a relationship based on R + any 1P 2
Able to make a relationship at idea level 1
No response or incorrect in response 0
1(h)Able to deduce about the activity of amylase on starch based
on the following aspects:
P1 : Amylase is the enzyme which hydrolysis the starch 3
P2: The colour of iodine change from yellow to blue-black
P3:
. Affected by the temperature
Sample answer
Amylase is the enzyme which hydrolyses the starch by changing the
colour of iodine from yellow to blue-black and reacts best at its
optimum temperature // 370C
Able to define based on any two aspects 2
Able to define based on any one aspects 1
No response or incorrect in response 0
1(i)Able to predict what will happen if the experiment is
repeated at 370C but changing the pH of the medium at 3.
R : Not hydrolysed 3
P1 : No colour change on iodine
P2:
. Amylase only can function in neutral condition/ pH 7
Sample answer
The starch will not be hydrolysed. So, no colour change on iodine. This
is because amylase only can function in neutral condition/ pH 7

Able to predict based on R + P1/P2 2

Able to predict based on R/P1 1
No response or incorrect in response 0
2(a) Problem statement
Able to state a problem statement relating the manipulated
variable to the responding variable correctly based on the
following aspects:
P1 : The concentration of the sucrose solution 3
.
P2: The mass of the potato strips
R: Question form
Sample answer
What is the concentration of sucrose solution which is isotonic to the
cell sap of potato strip?
Able to state a problem statement less accurately 2
Able to state an idea of a problem statement 1
No response or incorrect in response 0
2 Hypothesis
Able to state a hypothesis based on the following aspects:
P1 : The concentration of the sucrose solution 3
P2: The mass of the potato strips
R:
. Relationship of the variables
Sample answer
An isotonic sucrose solution will not change the mass of the potato strip
Able to state a hypothesis less accurately based on any two aspects 2
Able to state an idea based of a hypothesis on any two aspects 1
No response or incorrect in response 0
Variables
Able to state all three variables correctly.
Sample answer 3
MV: The concentration of the sucrose solution
RV:
. The mass of the potato strips
CV: Temperature of the surroundings
Able to state any two variables correctly 2
Able to state any one variables correctly 1
No response or incorrect in response 0
List of Apparatus and Materials
Able to list all the apparatus and materials correctly.
Sample answer 3
A razor blade, a cork borer, petri dish, forceps, electronic balance, dry
cloth,
. a potato, distilled water, (5%, 10%, 15%, 20%) sucrose solution
(6 apparatus + 3 materials)
Able to list at least 5 apparatus + 2 materials 2
Able to list at least 3 apparatus + 2 materials 1
No response or incorrect in response 0
Procedure
Able to describe the steps of the experiment procedure or methods
correctly based on the following aspects:
K1 : Preparation of the materials and apparatus 3
K2: Operating the constant variable
K3: .Operating the responding variable
K4: Operating the manipulated variable
K5: Steps to increase the accuracy of the results/ precautions
Sample answer
1.Five petri dishes are prepared and labelled 1 to 5.
2. The petri dishes are then filled with the solutions as shown in the table below.
Petri dishes Solution
1 Distilled water
2 5% sucrose solution
3 10% sucrose solution
4 15% sucrose solution
5 20% sucrose solution
3. A cork borer is pushed into a large potato to obtain a strip of potato which is
5cm long.
4. Four more strips of potato of the same size are prepared in the same way.
5. Each strip of potato is dried with a piece of cloth and its mass is measured by
using an electronic balance.
6. One strip of potato is then placed in each petri dish for 1 hour.
7. After soaking for 1 hour, each potato strip is removed and its mass is determined
by using electronic balance and recorded.
8. All the results are tabulated. A graph of the percentage change in mass against
the concentration of sucrose solution is plotted.
Scoring rubric
K1: Steps 1,2,3,5,6 (any 3) 3
K2: Steps 3,4,6 (any 2)
K3: Steps 7, 8 (any 1)
K4: Step 2
K5:
. Step 5
All the 5K
Any 3 – 5 K 2
Any 1 – 2 K 1
No response or incorrect in response 0
Presentation of data
Able to present all the data with the correct units based on:
P1: MV 3
P2:RV/ORV
Sample answer
.Petri Solution Mass of potato strip(g) Change in Percentage of
dish Initial Final mass (g) change in mass (%)

1 Distilled water
2 5% sucrose solution
3 10% sucrose solution
4 15% sucrose solution
5 20% sucrose solution

Able to state any P2 2

Able to state any P1 1
No response or incorrect in response 0