VIETNAM INTERNATIONAL UNIVERSITY

SCHOOL OF BIOTECHNOLOGY

Analysis of salt-stress effect on root length

and proline content of mung bean
BSc THESIS PROPOSAL DEFENSE

SUPERVISOR: Group members: Bui Quang Huy –BTBTIU13085

Ph.D Nguyen Thi Thu Hoai Nguyen Pham Quynh Anh – BTBTIU14018
Tran Phuong Quynh – BTBTIU15149
Pham Thi Ngoc Linh – BTBTIU15125
Dan Phuc Quang Tam – BTBTWE16016

Date of proposal: Friday, 23/03/2018

 I- INTRODUCTION

II- OBJECTIVES
OUTLINE
III- EXPERIMENTAL DESIGN AND
SCHEDULE
IV- REFERENCES
I- INTRODUCTION
 Mung bean (Vigna radiata L.) has been shown to
have antioxidant, antitumor, anti-inflammatory,
hepatoprotective, antimicrobial, diuretic, and
hypoglycaemic effects…
 Recently in Vietnam, the cultivating area for
mung bean tends to be reduced. One of the main
causes of this reduction is climate change and
global warming which leads to salt intrusion in
delta
 Reduction in cultivating area will lead to low
production of mung bean which are in high
supplying demand.
I- INTRODUCTION

 Salinity stress significantly reduces mung bean yield (Abd-Alla et

al., 1998; Saha et al., 2010) through decline in seed germination,
root and shoot lengths, fresh mass …(Promila and Kumar, 2000;
Misra and Dwivedi, 2004)
 Sodium chloride stress severely reduced the crude protein content
of plant parts. Water uptake also was substantially decreased
under stress conditions, particularly at the highest level of stress.”
(Mohammad Pessarakli, Jan 25, 2015)
 Hypothesis:
 There are significant effects of different
salinities on root length and weight of
mung bean
II- OBJECTIVES Project Goals:
 Screening homologous phenotypes
 Evaluate the effect of different salinities
on physiological traits of mung bean
III- EXPERIMENTAL DESIGN
1) Screening of
homologous mung
bean line
Experiment: Root growth
under different salinities

2) Evaluation of
salt-stress on
mung bean
Measurement of root
length and proline
content in the root
3) Data analysis
 Select the mung beans with similar
Part I: 
morphological appearance
Selection of a  Round shape (similar diameter and
homologous similar length)

mung bean line  Not wrinkle

 Green phenotypes
Part II:
 II.1: Soil box: There are 3 replicates for each
Evaluation of concentration  prepare 3 soil boxes for each
salt-stress concentration
  II.1:
• Use NaCl to make different salinities
(control, 5.0 dS/m, 10.0 dS/m, 20.0
Part II: dS/m)
Evaluation of • Each concentration will be replicated 3
times
salt-stress
• Experimental unit: 1 box of 5 beans
• Sampling unit: 1 bean
  II.2: Proline contents of the root:
• Prepare standard solution to construct
standard curve
• Proline colorimetric determination
proceeded according to Bates et al. (Bates
Part II: et al., 1973, Marín et al., 2009) based on
proline's reaction with ninhydrin. Using
Evaluation of spectrophotometer to measure OD
salt-stress • The concentration of proline in root tissues
was determined by triturating the frozen
roots.
• Based on the standard curve, calculate
proline content
Part III: Data  The length of the root and the concentration
of proline in different cultivars will be
analysis analyzed by ANOVA Two-way and further
multiple comparison test.
SCHEDULE:
Day Work
Day 1 • Prepare 5 different NaCl solution (….)
• Selection of 30 homologous mung beans
• Soaking the mung beans in warm water overnight for imbibition

Day 2 • Sow the beans in the prepared soil boxes and irrigating TAP
WATER into the soil. (20ML/TIMES, 3TIMES/DAY)

Day 3- Day 18 • Irrigating the beans with the same procedure in day 2 BUT
REPLACE TAP WATER WITH PREPARED SOLUTIONS
Day 18- Day 38 • Irrigating with 50mL/day
Day 21-25 • Take the measurement AT DAY 18 AND DAY 30
• Analysis
 REFERENCES:
 Mohammad Kafi & Zainab Rahimi (2011) Effect of salinity and silicon on root characteristics, growth, water status,
proline content and ion accumulation of purslane (Portulaca oleracea L.), Soil Science and Plant Nutrition, 57:2, 341-347.
 Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and
soil, 39(1), 205-207
 Marin, J. A., Andreu, P., Carrasco, A., & Arbeloa, A. (2010). Determination of proline concentration, an abiotic stress
marker, in root exudates of excised root cultures of fruit tree rootstocks under salt stress. Revue des Régions Arides, 24,
722-727
 Munns, 2005; Rozema and Flowers, 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651–681
 Amira M.S. Abdul Qados (2010). Effect of salt stress on plant growth and metabolism of bean plant Vicia faba (L.).
Journal of the Saudi Society of Agricultural Sciences,10, 7-15
 Ahmed, S. (2009): Effect of soil salinity on the yield and yield components of mungbean. Pak. J. Bot,41:263-268.
 Promila K., Kumar S. (2000). Vigna radiata seed germination under salinity. Biol. Plant. 43 423–426. 10.1093/pcp/pcs040
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