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Spectrofotometry Dan Penerapannya

Pada Laboratorium Klinik

Kelompok 4

Triswono Ramadhan Zarkoni


Chitriani Armidha
Abdullah Irfan Rahmadi
Siti Saenab
MATERI DISKUSI
01 SPEKTROFOTOMETER

02 KOMPONEN-KOMPONEN SPEKTROFOTOMETER

03 PRINSIP KERJA SPEKTROFOTOMETER

04 JENIS-JENIS SPEKTROFOTOMETER DAN


PENERAPANNYA DALAM LABORATORIUM KLINIK
SPEKTROFOTOMETER
Spektrofotometer merupakan alat yang
digunakan untuk mengukur absorbansi
dengan cara melewatkan cahaya dengan
panjang gelombang tertentu pada suatu ob
yek
kaca atau kuarsa yang disebut kuvet.

Spektrometer menghasilkan sinar dari spektru


m
dengan panjang gelombang. Spektrofotometer
digunakan untuk mengukur energi secara relatif
jika energi tersebut ditransmisikan, direfleksika
n atau
diemisikan sebagai fungsi dari panjang gelomb
PRINSIP SPEKTROFOTOMETER

01 SpektrumJika seberkas cahaya melewati


Elektromagnetik
Absorbsi cahaya mengakibatkan
Spektrum elektromagnetik dipisahkan transisi elektronik,
dalam beberapa
region berdasarkan
yaitu promosi panjang gelombang
elektron-elektron dariantara lainkeadaan
orbital X-ray
dasar yang
(0.01-10 berenergi
nm), sinar tampakrendah ke(380-760
(visible) orbital keadaan
nm) dan
02 Hukum Lambert-Beer
microwave (1-10cm).
tereksitasi Sedangkan
berenergi lebih pada
tinggi.spektrofotometer
Energi yang
UV/Vis mengukur
terserap pada panjang
kemudian terbuanggeombang
sebagaiUV (10-400
cahaya nm)
atau
dan visible (380-760 nm) dekat daerah infra-red
tersalurkan dalam reaksi kimia.
03 Absorbsi Cahaya (750-2250 nm).
warna yang Warna yang Panjang
teramati diserap gelombang

Green Red 700 nm

Blue-green Orange-red 600 nm Jika benda yang tidak tembus sinar menyerap
sebagian dari warna-warna spektrum sinar putih yang
menyinarinya dan memantulkan warna-warna yang
Violet Yellow 550 nm
lain, makabenda tidak tembus sinar itu akan tampak
bewarna. Warna yang tampak adalah warna (panjang
Red-violet Yellow-green 530 nm gelombang sinar putih) yang dipantulkan oleh benda
tersebut. Warna yang dipantulkan ini disebut warna
komplementer dari warna yang diserap oleh benda.
Red Green 500 nm
Warna dan warna komplementernya merupakan pasangan
dari setiap dua warna spectrum, yang menghasilkan sinar
Orange Blue 450 nm putih bila keduanya dicampurkan.

Yellow Violet 400 nm


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KOMPONEN
SPEKTROFOTOMETER
KOMPONEN SPEKTROFOTOMETER

SUMBER CAHAYA MONOKROMATOR SAMPEL DETEKTOR PEMBACA


Single and Double- beam instrument
Double-beam instrument
Double-beam digunakan pada panjang gelombang
190 sampai 750 nm. Double-beam instrument mempunyai dua
sinar yang dibentuk oleh potongan cermin yang
berbentuk V yang disebut pemecah sinar. Sinar pertama
melewati larutan blangko dan sinar kedua
Single-beam instrument secara serentak melewati sampel, mencocokkan
1. Harganya murah, dan mengurangi biaya yang ada foto detektor yang keluar menjelaskan
merupakan keuntungan yang nyata. perbandingan yang ditetapkan
2. Radiasi dari monokromator yang masuk secara elektronik dan ditunjukkan oleh alat pembaca.
didispersikan oleh prisma/ grating.
3. Untuk pengukuran sinar ultra violet
dan sinar tampak.
4. Panjang gelombang paling rendah
adalah 190 sampai 210 nm dan paling
tinggi adalah 800 sampai 1000 nm.
Jenis-jenis spektrofotometri serta
Aplikasi Penggunaannya

UV-VIS IR AAS

NMR MASSA
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