with Tomes’ process Ameloblast: Secretion of enamel matrix + Partial mineralization (30%)
Starts after a layer of dentin is deposited
Ameloblast cell: 1. More mitochondria, secretory granules 2. Well developed Golgi complex 3. Extensive cisterns of REE 4. Vacuoles, ribosomes, vescicles, microtubules
Synthesis of enamel protein:
mRNA carry message (nucleus cytoplasm)
ribosomes translate message
protein synthesized in REE
post-translation modification of protein
in Golgi complex
packing of protein into secretory granules
Transport of enamel protein:
secretory granules basal plasma membrane release into extracellular space (against newly formed dentin) by exocytosis Secretory ameloblast: Proximal & distal end are connected together by intercellular junctions Formation of Tomes’ process before secretion of enamel Amelogenesis 3. POST-SECRETORY STAGE Maturative stage Maturative stage Ameloblast: mineralization + maturation of enamel Morphological alterations: Ameloblast Ruffle ended: Brush border with foldings, introduce inorganic compounds Smooth ended: smooth border, reabsorb protein & water Slight reduction in height Decreased volume & organelle content Undergo Apoptosis: Programmed cell death Necrosis: Accidental/Induced cell death Protective stage Protective stage After completion of enamel formation Brush border of basal membrane becomes smooth Ameloblast: columnar cuboidal + REE 2-3 layers of stratified epithelium REE: a. Covers newly formed enamel b. Until tooth erupts Desmolytic stage Desmolytic stage REE: 1. Secrete collagenase enzyme destroy connective tissue b/w oral mucosa & erupting tooth 2. Facilitate tooth eruption 3. Central cells degenerate form a canal (for tooth eruption) Enamel matrix deposition Amelogenesis : Formation of enamel Mineralization of enamel matrix Enamel matrix deposition Occurs during secretory stage Secretion of enamel matrix: 20-30% of protein (gradually decrease) Enamel matrix (composition): 1. Enamel proteins Amelogenin (90%): proline Non-amelogenin (10%): Enamelin, Ameloblastin a) Promotes nucleation b) Regulates crystal growth 2. Sulfated glycol-conjugates 3. Proteinases: Cause degeneration of amelogenins Facilitate reabsorption during maturative stage (post-secretory stage) 4. NO collagen & keratin present Ameloblast: Flat basal region conical process at base (Tomes’ process) Tomes’ process The only site to interact with growing enamel surface Disappears before last phase of secretory stage (before surface layer of enamel is deposited) Matrix deposition + Mineral transport only go through TP Contains numerous secretion granules, microtubules, microfilaments, mitochondria Enamel secretion (2 sites): a. Proximal part: contacts adjacent ameloblasts, produce inter-rod enamel b. Distal part: surrounded by enamel matrix, produce enamel rod Responsible for rod structure of enamel
Sheath-like zone (Prism sheath):
1. Border b/w rod & inter-rod enamel 2. Distinguish both regions Enamel matrix deposition Amelogenesis : Formation of enamel Mineralization of enamel matrix