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OF PANCREAS
4 hours 1
SURGICAL PATHOLOGY OF PANCREAS – 4 hours
• C8
– Acute pancteatitis: etiology, phisiopathology, clinical findings,
evaluation, diagnosis, differential diagnosis,
complications(pancreatic pseudocyst), treatment.
– Chronic pancteatitis: etiology, phisiopathology, clinical findings,
evaluation, diagnosis, differential diagnosis, complications,
treatment.
• C9
– Malignant tumors of the pancreas: etiology, classification,
pathology, clinical findings, evaluation, diagnosis, complications,
treatment.
– Tumors of the Vater's ampulla: etiology, classification, pathology,
clinical findings, evaluation, diagnosis, complications, treatment;
– Tumors of the endocrine pancreas.
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SURGICAL PATHOLOGY OF PANCREAS
Penetrating Injuries
• Penetrating injuries to the pancreas are usually diagnosed in the operating room.
• The pancreas is located in the retro peritoneum, surrounded by other viscera and major vascular
structures.
• An isolated injury to the pancreas is unusual, major vascular injuries are seen in 40% to 50% of
patients with penetrating pancreatic injuries,
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SURGICAL PATHOLOGY OF PANCREAS
• Patients with penetrating pancreatic trauma have obvious indications for abdominal exploration.
• Preoperative serum amylase concentrations are not helpful; (elevated in 30% of patients with
penetrating pancreatic injuries).
Abdominal exploration,
• Signs of pancreatic injury include:
– knife or a projectile path that passes in proximity to the pancreas,
– a central hematoma in the upper abdomen, and
– injuries to the duodenum, vena cava, suprarenal aorta, or mesenteric vessels.
• In all these instances, the pancreas should be thoroughly explored:
• The anterior surface of the pancreas is visualized by entry into the lesser sac of the peritoneal
cavity by division of the gastrocolic ligament in a relatively avascular area to the left of the midline.
• The tail of the pancreas can be more fully visualized, especially in its posterior aspect, by
mobilization of the spleen and the tail of the pancreas as a unit.
• The posterior aspect of the body of the pancreas is visualized by development of the avascular
area at the inferior margin of the body and tail of the pancreas with a combination of sharp and
blunt dissection.
• The posterior aspect of the head of the pancreas can be exposed by an extensive Kocher
maneuver. In combination with entry into the lesser sac, this also allows for bimanual palpation of
the pancreatic head, with one hand placed on the anterior surface of the pancreas through the
hole in the lesser sac and the other hand placed behind the pancreas in the plane developed by
the Kocher maneuver.
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SURGICAL PATHOLOGY OF PANCREAS
• In the evaluation of penetrating pancreatic injuries, the key to operative management is the
determination of whether a ductal injury is present.
– Transduodenal intraoperative pancreatography has been recommended, but the use of this
technique in the identification of ductal injuries is controversial or dangerous.
– In certain circumstances, the use of intraoperative ERCP (endoscopic retrograde
cholangiopancreatography) eliminates this problem.
• Penetrating pancreatic injuries can be classified according to both location and severity.
• With respect to location, injuries can be subdivided into those of the
– head,
– body, and
– tail of the pancreas.
• With respect to severity, classification systems must be satisfactory to research applications but
also in the determination of the best treatment.
Classification
– Class I injuries are simple contusions of the pancreas;
– Class II injuries are lacerations of the parenchyma in the body or tail of the pancreas;
– Class III injuries are those with severe disruption of the head or body;
– Class IV injuries are those in which there is an associated injury to the duodenum.
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SURGICAL PATHOLOGY OF PANCREAS
• Class I injuries should be usually observed or simply drained externally. The type of drain used after
pancreatic injury is probably not important as long as adequate drainage is effected. This can be
accomplished with passive, sump, or closed suction systems.
• If drains are used, they should be left in place for at least 5 to 7 days to ensure that a drain tract
develops. The timing of drain removal should be based on both the amount and character of the
pancreatic drainage:
– Drain outputs in excess of 150 to 200 mL/day are suggestive of pancreatic fistula.
• The morbidity rate for patients with undrained pancreatic secretions is much greater than that for those
with drained pancreatic secretions.
• Class II injuries The treatment depends on the presence or absence of a ductal injury,
a determination that can be difficult to make.
• The argument for intraoperative pancreatography is made for class II injuries.
• The presence of a ductal injury in the head of the pancreas does not usually make a difference with
respect to treatment, because most of these injuries should be drained regardless.
• If a ductal injury is present in the body or tail of the pancreas, the appropriate treatment is resection of
the distal pancreas, whereas if no ductal injury is present, simple drainage is adequate.
• the difficulty lies in determining whether injuries to the body or tail of the pancreas do in fact include
ductal injury.
• Intraoperative pancreatography is helpful, regardless of the technique employed.
• Class III injuries of the body or tail of the pancreas, should be treated with a distal pancreatectomy.
Distal resection can include up to 80% of the gland if necessary.
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SURGICAL PATHOLOGY OF PANCREAS
• Class III injuries of the head of the pancreas should be drained. Resection of these injuries requires
internal drainage, near-total pancreatectomy, or pancreaticoduodenectomy. If the patient develops a
pancreatic fistula, the fistula can be controlled by the drains. If the fistula does not resolve with time, the
pancreas can be drained internally at a later date.
• Class IV injuries of the pancreas involve injuries to the duodenum as well as the pancreas.
– If the injuries to the duodenum and pancreas are simple, the duodenum can be repaired primarily,
and the pancreas can be drained, or a distal resection can be carried out if the pancreatic injury is
in the body or tail.
– For more complicated, combined injuries, pyloric exclusion can be done to minimize pancreatic
stimulation and protect the duodenal repair (see Duodenum).
– For massive injuries to the duodenum and head of the pancreas, pancreaticoduodenectomy with
reconstruction should be reserved for cases in which débridement of devitalized tissue results in a
de facto removal of the duodenum and head of the pancreas.
– Penetrating injuries to the ampulla of Vater may also require formal pancreaticoduodenectomy.
• Internal drainage of the pancreas has been suggested as a means of treating ductal injuries without the
need for resection of viable and functional pancreatic tissue.
• Distal pancreatectomy for traumatic injuries should be performed only after the pancreas has been
thoroughly mobilized and exposed.
• It is possible to perform a distal pancreatectomy without a concomitant splenectomy. This adds to
operative time and can increase the risk of bleeding, particularly if there is an associated injury to the
spleen treated with splenorrhaphy.
• Splenic salvage should be attempted, therefore, only in hemodynamically stable patients with minimal
or no associated intraabdominal or extraabdominal injuries.
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SURGICAL PATHOLOGY OF PANCREAS
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SURGICAL PATHOLOGY OF PANCREAS
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SURGICAL PATHOLOGY OF PANCREAS
Blunt Injuries
• The major difference between penetrating and blunt injuries of the pancreas concerns the diagnosis.
• Penetrating injuries are usually discovered on abdominal exploration for associated injuries, but
blunt injuries may occur in isolation, and the preoperative diagnosis can be difficult.
• Blunt pancreatic injuries are relatively rare, which increases the difficulty of diagnosis. So delays in
diagnosis of blunt injuries ranged up to several days, which induce an increased morbidity.
• The body of the pancreas lies directly anterior to the vertebral column and is vulnerable to crush injuries
when the anterior abdominal wall is forceably compressed, as can occur from a seat belt or a sharp blow
to the epigastrium. In such instances, the pancreas may be the only intraabdominal organ injured.
• A number of different means are available to make the diagnosis of blunt pancreatic injury:
– Physical examination of the abdomen is useful, but because of the retroperitoneal location of the
pancreas, the results can be misleadingly benign until a number of hours after injury.
– This emphasizes the importance of serial examinations.
– In most cases, the abdomen becomes progressively more tender to palpation during the first 24 to
48 hours after injury, and the need for abdominal exploration becomes more obvious.
– The physical examination of the abdomen is much less reliable in young children and in patients
with head injuries.
• The serum amylase concentration is elevated on admission in about 70% of patients with blunt
pancreatic injury.
• However, elevated serum amylase has a poor positive predictive value and also occurs in many patients
without pancreatic injury. The amylase concentration can be elevated because of trauma to other
organs, including the salivary glands and the ovaries.
• DPL(diagnostic peritoneal lavage) is of little help in the early diagnosis of pancreatic injury unless there
have been associated intraperitoneal injuries. The retroperitoneal location of the pancreas results in
minimal findings in the lavage fluid, and obtaining amylase concentrations in the lavage fluid is not
helpful.
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SURGICAL PATHOLOGY OF PANCREAS
• CT scan of the abdomen allows for visualization of the retroperitoneum, including the pancreas.
In the case of isolated injury to the pancreas, the sensitivity of the CT scan is at its lowest shortly after
injury.
Although it may be a good test for the diagnosis of pancreatic injury after a number of hours have
passed, immediate CT of the abdomen will miss some pancreatic injuries, particularly if expert
interpretation is not available.
• Finally, ERCP is a means of diagnosing pancreatic injury.
ERCP is an attractive diagnostic method because it is less invasive than abdominal exploration and
also provides information about the status of the ductal system, but there are several practical
disadvantages of the technique.
– Most of the studies that have reported successful use of ERCP have involved stable patients
studied hours to days after injury and sent to a referral center specifically because of suspicion of
a pancreatic injury.
– These patients are a selected group, quite different from patients who are freshly injured.
– ERCP is not universally available and, even in large centers, is often unavailable at the odd hours
necessary for early diagnosis in acutely injured patients.
– Many endoscopists are fearful of inducing an exacerbation of pancreatitis in patients with mild
pancreatic injuries lacking ductal involvement.
• To summarize,
• the early diagnosis of blunt pancreatic injuries, particularly if they occur in isolation, can be
extremely difficult, and
• no single test allows for an easy and reliable diagnosis.
• A combination of serial abdominal examinations and serum amylase determinations, CT scan,
and ERCP in selected patients is the best diagnostic strategy available.
• These studies should be combined, with a low threshold for operative intervention if a pancreatic injury
is suspected.
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SURGICAL PATHOLOGY OF PANCREAS
• Basic principles of exposure and operative management of blunt injuries of the pancreas are the
same as for penetrating injuries.
• In many instances of severe injury, the pancreas has already been transected by the trauma,
making the pancreatic resection somewhat simpler to carry out.
• Isolated injuries of the pancreas from blunt trauma also lend themselves to distal pancreatectomy with
splenic preservation.
• As in penetrating injury, splenic salvage should be attempted only in stable patients without
associated splenic rupture or severe associated intraabdominal or extraabdominal injuries.
• Complications of pancreatic injury are similar to those outlined for penetrating injuries.
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SURGICAL PATHOLOGY OF PANCREAS
ACUTE PANCREATITIS
DEFINITION
• Acute pancreatitis is a complex disorder of the exocrine pancreas characterized by acute acinar cell
injury and both regional and systemic inflammatory responses.
• It is a common disease with a broad spectrum of clinical and pathologic findings that contribute to
considerable morbidity and mortality.
• Because pathogenic mechanisms are unclear, specific treatment is not available.
Empiric supportive care remains standard, and clinical outcomes have improved only to the
extent that critical care has evolved in recent years.
Most patients with acute pancreatitis have simple edematous pancreatitis, a self-limited and reversible
process.
In a small number of patients, fulminant or progressive disease develops, with pancreatic necrosis
that can lead to multiorgan system failure or death.
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SURGICAL PATHOLOGY OF PANCREAS
ACUTE PANCREATITIS
PATHOLOGY
Normal acinar cell ultrastructure. Cytoplasmic processing of the proenzymes is depicted, with apical
discharge into the acinar ductule by means of zymogen granule exocytosis.
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SURGICAL PATHOLOGY OF PANCREAS
ACUTE PANCREATITIS
PATHOLOGY
• Acute pancreatitis is characterized by alterations in acinar cell structure and function as well as by
the development of acute regional and systemic inflammatory responses.
• The fundamental pathologic event is injury to the acinar cell.
• Acute pancreatitis is characterized by:
– Interstitial edema formation.
– Grossly, the gland becomes enlarged and edematous, with small areas of focal necrosis
involving either the pancreas or areas of adjacent retroperitoneal fat.
– Microscopically, edema is both interlobular and intralobular, and the acinar units appear
dispersed within the relatively sparse fibrous matrix.
ACUTE PANCREATITIS
• Acute inflammation occurs rapidly, experimentally, the process begins within minutes.
• The initial cellular response involves the infiltration of polymorphonuclear leukocytes into the perivascular
regions of the pancreas.
• Within hours, mononuclear cells, including macrophages and lymphocytes, accumulate.
• Experimental evidence suggests that phagocyte-derived oxygen radicals and possibly other phagocytic
products are involved in a primary injury to pancreatic capillary endothelial cells.
• The resulting increase in microvascular permeability facilitates access to the acinar cell microenvironment for
circulating formed elements (additional neutrophils, monocytes, platelets) and humoral factors, such as
complement products and cytokines.
– The relative importance of this inflammatory injury to the pancreatic microvasculature is unclear, but it
provides one explanation for the process of local edema formation as well as for the systemic
microvascular sequelae of acute pancreatitis.
• Although clinical
– Edematous pancreatitis usually is a reversible disease characterized by acinar cell injury and edema
formation,
– frank pancreatic necrosis develops in 5% to 10% of patients, which can lead to irreversible regional
injury or multiorgan system failure.
Predictably, this group of patients is the primary source of morbidity and mortality associated with acute
pancreatitis.
• Histologic characteristics of advanced disease include
– extensive acinar cell necrosis,
– interstitial microabscess formation,
– extensive peripancreatic fat necrosis,
– microvascular thrombosis, and
– local hemorrhage.
• Pathologically, all these features appear to represent progression of processes already established with
acute edematous pancreatitis. 16
SURGICAL PATHOLOGY OF PANCREAS
ACUTE PANCREATITIS
PATHOPHISIOLOGY
• The cellular events that lead to acute pancreatitis may be initiated by a variety of different stimuli, and
the process has been considered a final common pathway. Current data offer considerably more
insight into the relevant pathogenic events than the simple historic concept that pancreatic autolysis
occurs.
• Acinar cell proteases (such as trypsin, chymotrypsin, carboxypeptidase, and elastase) and
phospholipases are normally synthesized in an inactive zymogen form.
• Peptide synthesis is accomplished and the proenzymes are packaged into cytoplasmic zymogen
granules.
• After apical exocytosis into the acinar ductal lumen, these precursors are transported with water and
bicarbonate through the pancreatic duct into the duodenum, where they are converted
enzymatically into active forms by enterokinase, a brush-border enzyme.
• A variety of endogenous protease inhibitors (α1-antitrypsin, β 2-macroglobulin, and pancreatic
secretory trypsin inhibitor) are normally found in pancreatic tissue and pancreatic secretions, and in
plasma in quantities sufficient to protect against premature or inappropriate activation of these
digestive enzymes.
• The specific mechanisms that initiate human disease are not known, the normal orderly secretory
sequence appears to be disrupted in acute pancreatitis.
• Inappropriate protease activation overcomes endogenous antiprotease defenses.
• In experimental acute pancreatitis, zymogen granules become localized with lysosomes and fuse to
form autophagic cytoplasmic vacuoles (zymogen lakes). These vacuoles move preferentially to the
basolateral acinar cell cytoplasm, rather than to the luminal apex.
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SURGICAL PATHOLOGY OF PANCREAS
ACUTE PANCREATITIS
• Disordered discharge of the acinar cell contents through the basolateral cell membrane occurs.
• The mechanisms by which this cytoplasmic colocalization of zymogen granules and lysosomes occurs
are not known,
• Although evidence suggests that cytoskeletal alterations are associated with the loss of normal
acinar cell polarity and loss of the ability to achieve apical exocytosis.
• Trypsinogen is normally a major constituent of the zymogen granules, whereas cathepsin B is
usually abundant within the lysosomal fraction. Cathepsin B–induced trypsinogen cleavage is known
to generate activated trypsin, which, in turn, is capable of further cytoplasmic proenzyme conversion.
• The cytoplasmic liberation of activated proteases causes cell membrane injury, followed by the
disordered discharge of acinar cell contents through the basolateral cell membranes. For example,
intracellular trypsinogen cleavage has been demonstrated after either hypoxia- or acidosis-induced
acinar cell injury. Collectively, these findings suggest that the outcome of acinar cell injury involves
intracellular activation of endogenous proteases, leading to further injury and the local extracellular
discharge of acinar cell contents.
ACUTE PANCREATITIS
• Once initiated, this process of protease release perpetuates acinar cell injury and initiates a regional
acute inflammatory response, generating additional injury.
• The endogenous inflammatory system participates early in the development of acute pancreatitis.
• Both humoral and cellular factors / elements appear to be involved:
– Complement activation,
– Histamine release, and
– Bradykinin generation are demonstrable. Studies have suggested a role for
– Cytokines in the acute inflammatory process of pancreatitis.
– Serum levels of tumor necrosis factor (TNF) α, interleukin-6 (IL-6), and IL-1 are elevated in
animals with experimental pancreatitis and in patients with systemic complications of acute
pancreatitis. Administration of an IL-1 receptor antagonist, even after the onset of acute
pancreatitis, limited the degree of pancreatic inflammation. The administration of anti – TNF
antibody has both beneficial and deleterious effects on the development of local inflammation
and may reflect the different experimental models used.
– Neutrophil-mediated pancreatic capillary endothelial injury appears to occur early, when the
process is still reversible.
– NADPH oxidase–dependent oxygen radicals are directly implicated, whereas other phagocyte
products, such as elastase, collagenase, cathepsin G and D, phospholipases A2 and C, DNAase,
RNAase, glycosidases and other lysosomal hydrolases, platelet-activating factor, and
myeloperoxidase, are all potential participants in both the acinar and endothelial cell injury
processes.
– Chronic inflammatory cells, particularly macrophages, are recruited to the pancreas within hours
and share many of the proinflammatory products noted earlier. The microvascular injury
appears to amplify the inflammatory process
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SURGICAL PATHOLOGY OF PANCREAS
ACUTE PANCREATITIS
Schematic diagram illustrating the inflammatory response in acute pancreatitis. Inflammatory effector
cells and plasma and tissue mediators are depicted. Increased microvascular permeability results
from capillary endothelial cell injury.
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SURGICAL PATHOLOGY OF PANCREAS
ACUTE PANCREATITIS
• In addition to this localized pancreatic inflammation, evidence of a systemic response exists.
• Considerable data implicate the inflammatory process in the pathogenesis of pancreatitis-induced
multiorgan system failure.
• The systemic distribution of activated neutrophils, mononuclear cells and macrophages, complement
activation products, and other factors is clearly linked to remote organ dysfunction.
• A common event appears to be microvascular endothelial cell injury in diverse target organs.
• Pancreatitis-induced, polymorphonuclear leukocyte–dependent microvascular lung injury is one such
example This pathologic process is a likely explanation for the frequent pulmonary symptoms and the
occasional adult respiratory distress syndrome in patients with acute pancreatitis.
• Other target organs at risk for acute pancreatitis-induced injury are the liver, kidneys, and heart,
although the mechanisms involved are less clear.
• Complications of acute pancreatitis :
Early
– Shock
– Multiorgan failure
– Encephalopathy
– Coagulopathy
– Sepsis
– Hypocalcemia
Late
– Pseudocyst One consequence of the systemic
inflammatory response associated with
– Diabetes acute pancreatitis is neutrophil-
dependent, oxygen radical–mediated
– Abscess alveolar capillary endothelial cell injury. 21
SURGICAL PATHOLOGY OF PANCREAS
ACUTE PANCREATITIS
ACUTE PANCREATITIS
• The role of the microcirculation in the development of acute pancreatitis is poorly understood.
– In addition to increased permeability, microvascular thrombosis or obstruction by leukoaggregates may
lead to local or regional tissue hypoxia.
– In nonacinar cell populations, it is well known that hypoxia-induced adenosine triphosphate depletion is
associated with cytoskeletal (microtubule and microfilament) disruption.
– A similar finding in acinar cells would be consistent with the pathogenic scheme summarized earlier.
Diminished microvascular blood flow occasionally initiates the process of acute pancreatitis.
– Clinically, hypoxic acinar cell injury is thought to be associated with events such as cardiopulmonary
bypass, thromboembolic disease, and myocardial infarction.
Incidence
• The precise incidence of AP is difficult to determine.
• Variations among populations are highly dependent on social factors such as ethanol use and on
environmental and hereditary determinants such as the incidence of gallstones.
Age
• AP can occur at any age but is most common in adults between 30 and 70 years of age.
• Patients with gallstone-induced pancreatitis are older (age 40 to 60 years), whereas those with
alcohol-associated pancreatitis are younger (age 30 to 40 years).
Sex ratio
• The sex distribution of acute pancreatitis depends on the clinical cause of the disease:
– women represents 68% of patients with gallstone-associated pancreatitis
– when alcohol is the primary association, most patients are men.
Mortality
• The mortality rates associated with AP range from 6% to 20.5%.
• Acute hemorrhagic or necrotizing pancreatitis is associated with mortality rates of 50% or more.
Necrotizing pancreatitis occurs in 5% to 10% of patients in most series of acute pancreatitis.
• More recent studies have reported lower mortality rates in AP, reflecting advances in critical care,
nutritional support, and antibiotic therapy.
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SURGICAL PATHOLOGY OF PANCREAS
ETHANOL
OTHER:
• Thiazides
• Trauma
• Ethacrynic acid
• Postprocedural
• Diazoxide
• Postoperative
• Calcium
• Post-ERCP
• Coumadin
• Direct
• Cimetidine
• Mechanical (nongallstone) obstruction
• Quinidine
• Tumors of the pancreas, duodenum, or bile duct
• Phenformin
• Duodenal obstruction
• Azothioprine
• Pancreas divisum
• Mercuric chloride
• Infection
• Paracetamol
• Hyperlipidemia
• Sulfonamides
• Hyperparathyroidism
• Tetracyclines
• Drugs
• L-Asparaginase
• Steroids
• Methyldopa
• Estrogen
• Clonidine
• Glucocorticoids
• Pregnancy
• Diuretics
• Idiopathic
• Furosemide
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SURGICAL PATHOLOGY OF PANCREAS
• In one review of 1450 patients with gallstone-associated pancreatitis shows the following aspects:
– gallstone impaction at the ampulla of Vater was identified in only 2%.
– Simple cholelithiasis was found in 72%,
– choledocholithiasis in 20%, and
– cholecystitis without apparent gallstones in 8%.
• Presumably, passage of a gallstone before diagnostic evaluation is routine. When stools are carefully screened
in patients with suspected biliary pancreatitis, gallstones can be demonstrated in 85% to 94% of the patients within 10 days of the onset of
disease.
Ethanol
• Ethanol use is the most common cause of AP in the United States.
• In addition to the pathophysiologic mechanisms proposed earlier, it is likely that genetic, dietary, and
environmental factors contribute.
• Pancreatic ductal hypertension is probable after
ethanol ingestion and this may occur by several
different mechanisms:
– The production and precipitation of protein
(stone protein) within the pancreatic duct
Hyperlipoproteinemia
• Rare causes of AP are the hyperlipoproteinemias, types I and V. The initial acinar cell injury is thought
to result from the liberation of free fatty acids from circulating triglycerides by the local action of lipases
within the pancreatic microcirculation. Physical disruption of the microvascular endothelium by
cholesterol crystals may also occur.
Hyperparathyroidism
• AP is reported in 1% to 19% of patients with hyperparathyroidism. It is unclear whether increased
circulating plasma levels of calcium or parathyroid hormone are primarily responsible.
Hypercalcemia may be associated with the precipitation of calcium phosphate within the pancreatic
duct as well as with pancreatic hypersecretion.
Parathyroid hormone elevated levels may have a direct cytotoxic effect on acinar cells.
Drugs
• Drug-induced acute pancreatitis is relatively common and has been reported in association with many
pharmacologic agents. The mechanisms involved are largely unknown.
Infections
• The development of acute pancreatitis has been reported after a variety of bacterial, fungal, parasitic,
and viral infections. Postulated mechanisms include direct cytotoxic effects, coexisting
immunosuppression, and alterations in pancreatic blood flow.
Vascular Disease
• Impaired pancreatic blood flow arising from either anatomic lesions or functional events can induce
acute pancreatitis. IE: embolization of the pancreaticoduodenal artery after translumbar aortography,
celiac artery stenosis, ruptured abdominal aortic aneurysm, and myocardial infarction.
Immunologic Factors
• AP has been associated with systemic lupus erythematosus, rheumatoid arthritis, polyarteritis nodosa,
and Be87het syndrome. Systemic vasculitis, the associated glucocorticoid use, and the presence of
circulating antibodies to acinar cells are potentially related findings.
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SURGICAL PATHOLOGY OF PANCREAS
On Admission
• Age above 55 years
• White blood cell count above 16,000/E6L
• Glucose level above 200 mg/dL
• Lactase dehydrogenase level above 350 IU/L
• Serum glutamic-oxaloacetic transaminase value above 250 IU/L
After 48 Hours
• Hematocrit decrease of 10%
• Blood urea nitrogen level increase of 5 mg/dL
• Ca2+ level below 8 mg/dL
• PaO2 level below 60 mmHg
• Base deficit value above 4 mEq/L
• Fluid sequestration greater than 6 L
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SURGICAL PATHOLOGY OF PANCREAS
DIAGNOSIS
• The diagnosis of AP ultimately depends on a clinical judgment, based on the finding of epigastric
abdominal pain and tenderness with the laboratory finding of hyperamylasemia.
• No single laboratory or physical finding is pathognomonic.
Imaging
• Plain abdominal roentgenograms may show :
– A diffuse ileus and
– A solitary left upper abdominal sentinel loop are classic and are often seen on,
– The psoas muscle margins may be obscured by retroperitoneal edema;
– Pancreatic ascites may be apparent;
– Pancreatic calcifications imply preexisting chronic disease.
• Chest radiographs abnormalities are present at one third of patients with AP at the time of diagnosis.
– Segmental atelectasis,
– An elevated hemidiaphragm,
– Pleural effusions, or the presence of
– Early pulmonary parenchymal infiltrates.
• Barium studies of the gastrointestinal tract An upper gastrointestinal contrast study often demonstrates
– narrowing or spasm of the duodenum, with
– widening of the C loop secondary to pancreatic inflammation and edema formation.
• Ultrasound scanning provides a readily available, rapid, and noninvasive imaging of the pancreas.
– In the case of simple acute pancreatitis, an enlarged, echolucent gland is typically seen.
– Ultrasound examination yields information regarding cholelithiasis, choledocholithiasis, and the
status of the intrahepatic and extrahepatic biliary ducts.
– The technique is also valuable for assessing and sequentially evaluating peripancreatic fluid
collections or pancreatic pseudocysts. 33
SURGICAL PATHOLOGY OF PANCREAS
• Computed tomography (CT) has similar capabilities to ultrasound, although sensitivity for detecting
cholelithiasis is lower. Intraluminal contrast in the duodenum and small bowel is necessary to optimize
CT imaging. Dynamic CT scanning with simultaneous intravenous contrast enhancement can give
valuable information in evaluating regional pancreatic perfusion and may provide an estimate of the
extent of pancreatic necrosis. CT may show gas bubbles in the pancreatic necrosis or peri pancreatic
fluid collections assessing the infection though the indication for surgical approach
• Needle aspiration either CT- or ultrasound-guided, allows access to peri pancreatic or pancreatic fluid
collections for diagnostic sampling and/or therapeutic drainage. Cultures may be obtained by this
route. Contrast injections can assess the relation of a pseudo cyst or abscess cavity to the pancreatic
duct. This latter information is important for any preoperative planning. Placement of therapeutic
drainage catheters is routinely combined with CT- and ultrasound-directed imaging procedures.
• ERCP The role in the diagnostic evaluation of the patient with AP is severely limited because of the
high risk of exacerbating existing inflammation. ERCP is generally reserved for the evaluation of a
patient with a suspected obstructive lesion and is timed to follow resolution of the acute phase of the
illness. In addition, it is appropriate to obtain ERCP in a patient with idiopathic acute pancreatitis after
a first recurrence of the disease. This strategy is designed to identify promptly anatomically
correctable causes of acute pancreatitis. ERCP is also useful to delineate the pancreatic duct after
injury, pseudo cyst drainage, or the development of pancreatic ascites. Lastly, ERCP combined with
urgent therapeutic sphincterotomy and gallstone extraction has been used for patients with impacted
ampullary gallstones.
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SURGICAL PATHOLOGY OF PANCREAS
Biochemical Markers
• Amylase. Amylase is released from the acinar cell into the pancreatic microcirculation in conjunction
with the pathophysiologic events described earlier.
• The laboratory finding of hyperamylasemia in a patient with clinical signs and symptoms of AP is the
usual means of confirming the diagnosis of AP.
• Efforts to correlate the degree of hyperamylasemia with disease severity or prognosis have been
consistently unsuccessful, and Ranson criteria are notable for the absence of serum amylase levels.
An important reason for this relates to:
– the relatively rapid clearance of amylase from plasma,
– the half-life being about 130 minutes.
– Pancreatitis resulting from a discrete event such as transient pancreatic duct obstruction with
gallstone passage is characterized by a single serum amylase peak with a rapid rise and prompt
clearance, both measured in terms of hours.
• A normal or minimally elevated serum amylase level may also be found in a patient with necrotizing
pancreatitis or with CP; in these instances, complete or nearly complete destruction of the acinar cell
population may have occurred, reducing the plasma amylase level.
• Additionally, a number of nonpancreatic sources of amylase exist, so that hyperamylasemia may
result from other pathology:
– Salivary glands,
– fallopian tubes, and
– the small bowel are important alternative amylase sources.
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SURGICAL PATHOLOGY OF PANCREAS
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SURGICAL PATHOLOGY OF PANCREAS
• In the case of salivary gland disease, plasma amylase isoenzyme determinations differentiate the
source. An accurate and more rapid amylase assay using a monoclonal antibody specific to salivary
isoamylase has been described.
• Gastrointestinal tract pathology other than pancreatitis may lead to increased amylase absorption
through the intestine or peritoneum and relatively mild elevations of pancreatic amylase in the
circulation.
• Lipase. Lipase is derived primarily from pancreatic acinar cells, and its elevation is also taken as
evidence of acinar cell injury in acute pancreatitis. Lipase is also nonspecific and has not proved more
useful than serum amylase determinations in clinical use.
• Other Serum Enzymes. Other acinar cell products, such as immunoreactive trypsin, chymotrypsin,
elastase, ribonuclease, and phospholipase A2, may be detectable in plasma after the onset of acute
pancreatitis. Measurement techniques are not in wide clinical use, but institutional enthusiasm for one
particular assay or another has led to an abundant literature.
• Ribonuclease and phospholipase A2 plasma elevations may correlate with more complex disease.
• Methemalbumin. Methemalbumin results from the proteolytic conversion of hemoglobin into oxidized
hematin that is conjugated with plasma albumin.
Acinar cell protease release into the circulation increases red blood cell exposure to proteases and
accelerates this process, so that methemalbumin plasma levels may be elevated with acute
pancreatitis. A correlation between levels of methemalbumin and severity of pancreatic disease is
proposed but unproved.
• Other Serum Abnormalities. Other characteristic but nonspecific biochemical features commonly
associated with acute pancreatitis are summarized in the following Table These may have both
therapeutic and diagnostic relevance.
37
SURGICAL PATHOLOGY OF PANCREAS
Other characteristic but nonspecific biochemical features commonly associated with acute pancreatitis
INCREASED
Hematocrit, hemoglobin (hemoconcentration)
White blood cell count
Blood urea nitrogen
Creatinine
Bilirubin
Lipid, triglyceride levels
Glucose
Alkaline phosphatase
SGOT, SGPT
DECREASED
Hematocrit, hemoglobin (hemorrhage)
Calcium
Magnesium
Pao2
OTHER
Respiratory alkalosis (early)
Metabolic alkalosis (early)
Consumptive coagulopathy
Metabolic acidosis (late)
Respiratory acidosis (late)
38
SURGICAL PATHOLOGY OF PANCREAS
ACUTE PANCREATITIS
DEFINITION
• Acute pancreatitis is a complex disorder of the exocrine pancreas characterized by acute acinar cell
injury and both regional and systemic inflammatory responses.
• It is a common disease with a broad spectrum of clinical and pathologic findings that contribute to
considerable morbidity and mortality.
• Because pathogenic mechanisms are unclear, specific treatment is not available.
Empiric supportive care remains standard, and clinical outcomes have improved
only to the extent that critical care has evolved in recent years.
Most patients with acute pancreatitis have simple edematous pancreatitis, a self-limited and reversible
process.
In a small number of patients, fulminant or progressive disease develops, with pancreatic necrosis
that can lead to multiorgan system failure or death.
39
SURGICAL PATHOLOGY OF PANCREAS
MANAGEMENT
• Neither medical nor surgical treatment strategies provide specific therapy for the acinar cell injury
characteristic of acute pancreatitis.
• Modern approaches provide general supportive care in the form of:
– appropriate resuscitation,
– nutrition, and
– ventilation
in the expectation that the cellular pathophysiologic processes will be self-limited.
Medical Treatment
• Conventional medical therapy for AP consists fundamentally of:
– intravenous fluid resuscitation,
– nasogastric decompression, and
– monitoring of hematocrit, electrolytes, and blood gases.
• All are empiric and are not known to shorten or favorably alter the course of the disease.
• Regional retroperitoneal inflammation and the systemic microvascular injury contribute to the loss of
intravascular plasma volume.
• Hypovolemia may be mild or profound to the point of shock.
• A relation exists between the magnitude of extravascular fluid sequestration and the severity of
the pancreatitis.
• This is recognized by inclusion of an estimated plasma volume deficit exceeding 6 L in the
Ranson scoring system (see earlier list) as a grave prognostic sign.
• More than half of patients with acute pancreatitis have clinical evidence of inadequate end-organ
perfusion.
• Resuscitation requires the intravenous administration of large volumes of isotonic crystalloid
solution and the aggressive use of invasive hemodynamic monitoring devices.
40
SURGICAL PATHOLOGY OF PANCREAS
– Nitrogen losses of as much as 40 g/dl have been measured in patients with acute pancreatitis.
– Nutritional support is a necessary feature of their care.
Nutritional support To be initiated immediately after the acute resuscitation phase because of the
unpredictable return of intestinal function and the extraordinary metabolic requirements.
• Considerable debate has surrounded the selection of a nutritional route.
• The choice of route is less important than the need to provide adequate calories and to establish positive
nitrogen balance.
Antibiotic therapy is reserved for specific infectious complications such as pneumonia or pancreatic
abscess.
• Antibiotic use for simple acute edematous pancreatitis IS CONTRAINDICATED. Prospective randomized
studies in patients with simple acute pancreatitis using ampicillin, lincomycin, and cephalothin have
shown neither improvement in the clinical course nor a reduced likelihood of septic complications.
Specific metabolic complications such as hypokalemia, hypocalcemia, hemorrhage, and consumptive
coagulopathy are treated with appropriate replacement products, such as potassium chloride,
intravenous calcium gluconate or chloride, red blood cells, and fresh-frozen plasma.
• Hyperglycemia and glycosuria are the manifestations of altered carbohydrate metabolism in these
patients. Hyperglycemia occurs in about 10% of patients and is generally a transient phenomenon.
Permanent residual diabetes mellitus is much less frequent, occurring in fewer than 2% of patients.
• Treatment for the acute illness consists of the carefully titrated administration of exogenous
glucose and insulin to maintain a euglycemic state.
42
SURGICAL PATHOLOGY OF PANCREAS
A variety of pharmacologic agents that directly or indirectly reduce acinar cell enzyme release or ductal
secretion have undergone clinical evaluation for the treatment of acute pancreatitis, generally with
unimpressive results.
• Among the first were anticholinergic drugs, pancreatic anti enzymes, a somatostatin analogue.
• Despite the theoretic appeal, it has not been possible to demonstrate that pancreatic anti enzymes
and somatostatin alters the natural history or prognosis of simple acute pancreatitis, although it
diminishes pancreatic secretion.
Surgical Therapy
Surgical therapy for AP is reserved for specific complications and for those situations in which a
correctable anatomic cause can be identified.
Endoscopic sphincterotomy is usually the initial therapeutic procedure for relief of biliary ductal
obstruction when acute pancreatitis occurs in association with choledocholithiasis.
• The procedure reliably decompresses the ampulla of Vater, with overall clinical success for gallstone
disimpaction or passage in 90% of patients.
• ERCP has greatly reduced the need for operative procedures designed to either divert or open and
explore the common bile duct.
• In the more usual circumstance, a patient who has simple cholelithiasis and an episode of acute
pancreatitis is treated nonoperatively(ERCP) with resolution of the AP but gallbladder litiasis must be
operated. Cholecystectomy is often performed after the resolution of acute pancreatitis but before
hospital discharge.
44
SURGICAL PATHOLOGY OF PANCREAS
Surgery of Anatomically correctable lesions that can cause acute pancreatitis include:
– pancreas divisum,
– choledochal cysts (particularly a type III cyst or choledochocele), and
– pancreatic duct obstruction related to tumor, stricture, or injury.
• In the obstructive category, many patients have CP or recurrent acute pancreatitis.
• Surgical therapy is directed at achieving adequate pancreatic duct drainage, usually by diversion into
the jejunum for benign obstructions, or tumor resection in malignancies if appropriate.
• Symptomatic pancreas divisum is best treated with operative transduodenal sphincteroplasty,
• The type III choledochal cyst should be marsupialized into the duodenum.
• Acute pancreatitis may be clinically indistinguishable at presentation from an acute abdomen related to
other pathology :
– Perforated duodenal ulcer,
– Acute appendicitis,
– Ruptured abdominal aortic aneurysms
are sources of erroneous diagnoses.
• Virtually every experienced surgeon has performed an exploratory laparotomy for clinical evidence of
peritonitis only to find simple acute edematous pancreatitis.
In this situation, recognition of the correct diagnosis is crucial.
It is important to avoid biopsy, resection, or other nontherapeutic procedures that carry significant
risks.
In the event that devitalized tissue or saponified retroperitoneal fat are present, careful débridement
and external drainage may be appropriate.
• Peritoneal lavage as a specific therapy for acute pancreatitis was proposed after experimental
studies demonstrated improved survival in animals with fulminant pancreatitis.
45
SURGICAL PATHOLOGY OF PANCREAS
• The concept was appealing in that activated proteases and other vasoactive substances identifiable in
peritoneal aspirates from patients with pancreatitis would be removed, rather than systemically
absorbed.
• Unfortunately, clinical trials using this approach have produced disappointing results, and the eventual
overall mortality rate appears unchanged.
• Surgical approach for patients with necrotizing pancreatitis.
• When pancreatitis does not resolve spontaneously, diagnostic efforts are directed at distinguishing
infected and noninfected areas of pancreatic necrosis.
• The presence of an infected sequestrum mandates operative exploration to débride devitalized tissue
and to provide external drainage.
• Specific antibiotic coverage is essential and should be dictated by intraoperative cultures or aspirates
from the necrotic tissue.
• Débridement is often required on multiple occasions, usually at 24- to 48-hour intervals, until the
necrotic tissue is replaced by a granulating wound. Many strategies related to multiple operations with
open and closed peritoneal drainage systems have been devised.
• This is often a difficult judgment and is necessarily individualized for each patient.
46
SURGICAL PATHOLOGY OF PANCREAS
Pancreatic Pseudocysts
• A pancreatic pseudocyst is a fluid-filled cystic structure without a true epithelial lining that is associated
with the pancreas or pancreatic duct
• True cysts of the pancreas (epithelium-lined) are rare, whereas pseudocysts are relatively common.
• Pancreatic pseudocysts account for 2% to 10% of patients with pancreatic disease.
• The most common cause of pancreatic pseudocyst in the United States is ethanol-related CP. Biliary
and posttraumatic pancreatitis follow in frequency with regard to cause.
• The pseudocyst wall is composed of displaced adjacent viscera (often stomach, small bowel, or colon)
and a fibrous capsule that has evidence of both acute and chronic inflammation.
• The thickness of this fibrous capsule is variable, depending on how long the pseudocyst has been
present.
• The presence of a fibrous capsule becomes an important consideration in the timing and selection of
drainage procedures.
• The fluid within the cyst cavity is usually serous in character and contains pancreatic secretions,
including amylase and proteases, as well as albumin and inflammatory cells. The amylase content
may be high; it is not unusual to see a level of several thousand international units in pseudocyst
aspirates. If recent hemorrhage has occurred, bile pigment may also be present, and bacteria are
cultured in about 35% of pseudocysts.
The clinical presentation of a pancreatic pseudocyst is usually that of:
– Persistent visceral pain
– Ileus after an episode of acute pancreatitis.
– Fever,
– Leukocytosis,
– Palpable epigastric mass are common,
– Nausea and vomiting
– Jaundice suggests common bile duct obstruction from either intrinsic stones or extrinsic
distortion. 48
SURGICAL PATHOLOGY OF PANCREAS
• Most pseudocysts are unilocular and located in the head of the pancreas.
•
LOCATION OF PANCREATIC PSEUDOCYSTS
• Pseudocysts can dissect essentially anywhere within the retroperitoneal space. Therefore, a variety of
unusual presentations may occur, including intrathoracic or intraabdominal mass lesions.
• A left-sided pleural effusion is classic on chest radiograph. Splenic vein or portal vein thrombosis may
occur with pseudocyst formation, resulting in so-called left-sided portal hypertension and bleeding
esophageal varices.
• The management of pancreatic pseudocysts is based on whether the cyst is symptomatic.
• If the cyst is small (less than 5 cm), and the patient does not have symptomsthe pseudo cyst can be
safely observed; many of these resolve over a period of weeks.
• If the pseudocyst size greater than 5 cm the presence of a multilocular or debris-filled pseudocyst
cavity, and chronicity (a evolution longer than 6 weeks) associated with concurrent chronic alcoholic
pancreatitis are all factors that are associated with a lower probability of spontaneous resolution.
• ERCP is indicated to determine the pancreatic ductal anatomy:
– A pancreatic duct that communicates with the pseudocyst generally requires operative
management. In some institutions, endoscopic placement of pancreatic stents to bridge the
ductal disruption into the pseudocyst has been tried with moderate success.
49
SURGICAL PATHOLOGY OF PANCREAS
• The internal drainage procedure selected depends on the location of the pseudocyst and whether
there is associated pancreatic ductal pathology.
• Cystogastrostomy is the simplest and safest alternative if the pseudocyst is appropriately adjacent to
the posterior wall of the stomach.
• Cystoduodenostomy, using a transduodenal approach
• Cystojejunostomy using a Roux-en-Y or loop jejunostomy may also be appropriate, depending on the
location and specific anatomy of the pseudocyst.
Pancreatic resection is associated with the lowest recurrence rate (3%), but is limited to pseudocysts
occurring in the tail of the pancreas.
Cystojejunostomy Roux en Y
52
SURGICAL PATHOLOGY OF PANCREAS
Pancreatic Abscess
• The common causes of pancreatic abscess are:
– an infected pancreatic pseudocyst and
– necrotizing pancreatitis.
• The diagnosis is suggested by
– Persistent fever,
– Leukocytosis,
– A palpable abdominal mass.
– Bacteremia and systemic toxicity are late clinical features.
– On imaging, Ultrasounds, and/or CT scans: debris within a cyst is more suggestive of an abscess;
– Ultrasound-guided needle aspiration of suspicious peripancreatic fluid collections.
– Percutaneous aspiration with positive cultures is the definitive preoperative test.
• The treatment of choice is wide surgical debridement with removal of all infected and devitalized
tissues. Generous drainage is mandatory.
• Whether to leave the abdomen open and the choice of drainage systems remain controversial.
– Advocates of closed drainage (ie, placement of large, dependent drains and abdominal closure)
report mortality rates of about 30%.
– Use of open drainage or marsupialization with frequent dressing changes has a reported mortality
rate of 10% to 15%.
• The important principles are:
– to employ aggressive (often sequential) débridement,
– appropriate antibiotics, and
– effective external drainage.
53
SURGICAL PATHOLOGY OF PANCREAS
54
SURGICAL PATHOLOGY OF PANCREAS
Definition
CP is a disease characterized by progressive and permanent destruction of the pancreatic exocrine
parenchyma associated with fibrosis of the gland.
• In acute pancreatitis the lesions such as edema, hemorrhage, and fat necrosis, may regress
completely when the underlying cause is eliminated.
• Although both acute and CP may be categorized into relapsing and nonrelapsing forms depending on
their clinical presentation,
• Progressive morphologic and functional derangement is demonstrated only by CP.
• In CP, fibrotic destruction of the exocrine gland is often also accompanied by endocrine dysfunction.
Classification
• The classification of pancreatitis is usually reduced to include only acute and chronic disease.
• Within CP, calcifying CP and obstructive CP may be distinguished, with important functional and
therapeutic implications.
Incidence
• The incidence and prevalence of CP, is not known with precision.
• In the United States and Western Europe, the incidence of new cases approximates 5 to 10 per
100,000 population per year, with a prevalence of about 25 cases per 100,000 inhabitants.
• Because alcohol consumption is the most important risk factor for the development of CP, countries
with low alcohol consumption rates generally have lower incidence rates of CP. Correlation of alcohol
intake with incidence of CP within various populations has often been discrepant, however, suggesting
that environmental or hereditary factors may also influence susceptibility to the disease.
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SURGICAL PATHOLOGY OF PANCREAS
Alcohol Consumption
• Alcohol consumption is the major cause of CP, with about 70% of cases attributable to this factor.
• Most patients have consumed large volumes of alcohol for long periods of time.
• The average daily intake of alcohol was 150 to 175 g, and the risk for development of CP increased
with increasing alcohol intake.
• The mean duration of alcoholism before recognition of CP was 18 years for men and 11 years for women.
• Because only 10% of alcoholics develop CP, however, factors other than long-term alcohol exposure
may influence susceptibility.
• Diet may be important in this regard, the risk of alcohol-induced CP is increased by high-protein, high-
fat diets.
Heredity
• The hereditary form of CP is transmitted as an autosomal dominant trait of incomplete penetrance.
• Affected patients usually become symptomatic in childhood at an average age of 10 to 12 years.
• The clinical and histologic features of hereditary pancreatitis differ little from nonhereditary forms of the
disease.
• The diagnosis may be made if several members of a family develop CP in the absence of alcohol
consumption or other known causes.
Hyperparathyroidism
• Calcifying CP may occur in the presence of long-standing untreated hyperparathyroidism.
• Hyperparathyroidism is detected and treated at an early stage in Western countries,so the incidence of
associated CP is decreasing, currently accounting for not more than 1% to 2% of cases.
• The pathogenesis of CP in hyperparathyroidism is presumed to be due to injury caused by:
– The acute elevation of serum calcium which is a potent secretagogue for human pancreatic enzymes.
– Intraductal precipitation of calcium in pancreatic secretions
56
SURGICAL PATHOLOGY OF PANCREAS
Tropical Pancreatitis
• Tropical CP is a nutritional disease of importance in tropical Africa and Southeast Asia. The disease develops
among juveniles and young adults in the setting of chronic malnutrition. Protein-calorie malnutrition and
deficiencies of copper, zinc, and selenium have been associated with the disease. The precise cause remains
elusive.
Duct Obstruction
• Obstruction of the main pancreatic duct can cause a distinctive form of chronic pancreatic disease known as
obstructive pancreatitis. Occlusion may be caused by:
– tumors,
– congenital anomalies,
– scars from prior injury or
– inflammatory disease, or fibrosis of the ampulla of Vater.
• Although the gross alterations of the pancreas observed in obstructive pancreatitis are similar to those of
other forms of CP, microscopic changes are different :
– Obstruction causes diffuse atrophy of the exocrine tissue, whereas patchy atrophy is more common in
early forms of nonobstructive CP.
– Alternating areas of ductal stenosis and dilation are not seen; instead, the ductal system behind the
obstruction is uniformly dilated.
– The ductal epithelium is preserved, and protein plugs and calcifications are unusual.
– The relief of obstruction can be followed by reversal of parenchymal fibrosis and atrophy. A restitution of
pancreatic structure and function is not observed in other forms of CP.
• Obstructive CP is unusual, accounting for not more than 5% of cases.
Idiopathic Causes
• The most common form of nonalcoholic calcifying pancreatitis is idiopathic, a designation given to those
cases with an unrecognized cause. Idiopathic pancreatitis accounts for about 15% of cases.
• Idiopathic pancreatitis has two peaks in incidence, suggesting that differing underlying causes may exist:
– The first peak occurs in young adulthood;
– The second type, termed senile pancreatitis, has a peak occurrence at 60 years of age. 57
SURGICAL PATHOLOGY OF PANCREAS
• A novel protein has been identified in the pancreatic secretions of normal controls and patients with
CP.
• The association of this protein with pancreatic calculi led to its original designation as pancreatic stone
protein.
• Pancreatic stone protein is now referred to as lithostathine. This phosphoglycoprotein has a molecular
weight of 14,000. It has been localized immunohistochemically to zymogen granules in acinar cells,
suggesting an exocrine secretory pathway paralleling digestive enzymes.
• Lithostathine is hydrolyzed by trypsin and cathepsin to lithostathine H1 and H2.
• Lithostathine H1 acts to inhibit pancreatic stone formation. Lithostathine has the unique property of
suppressing nucleation of calcium carbonate. Pancreatic secretions normally contain calcium at
supersaturated concentrations, and the function of lithostathine is presumed to be inhibition of calcium
carbonate crystal formation. Low levels of this protein could thus have a major influence on the
development of calcific CP.
• Some patients with CP have an elevated pancreatic ductal pressure. Sphincter of Oddi manometry is
usually normal in such patients, suggesting that sphincteric dysfunction is not the cause of ductal
hypertension.
59
SURGICAL PATHOLOGY OF PANCREAS
Pain
• Pain is the predominant symptom in most patients with CP. Pain associated with CPis usually localized
to the epigastrium, with radiation to the back in the region of the upper lumbar vertebrae (Fig.).
• The pain is usually dull rather than sharp and constant rather than intermittent or colicky. Radiation to
areas other than the back is distinctly unusual.
• The discomfort may occasionally be alleviated by bending forward and is worsened by the supine
position.
• Ingestion of food or alcohol exacerbates the pain in many
patients, usually immediately after eating.
• Most patients experience pain daily; occasionally, painful
attacks are interposed by several pain-free days.
• The mechanisms responsible for pain in CP are incompletely
understood. Possibilities include:
– inflammation of the gland,
– damage to intrapancreatic nerves,
– increased pancreatic interstitial and intraductal
pressure
– associated conditions such as pseudocysts, bile duct
stenosis, or duodenal obstruction.
• Intrapancreatic neural inflammation may be observed
histologically in CP.
Topographic locations of pancreatic pain. • Intrapancreatic nerves usually remain viable while the
parenchyma is replaced by fibrosis,
60
SURGICAL PATHOLOGY OF PANCREAS
• In fact, subclinical endocrine defects are common in the early stages of CP:
– Altered insulin secretion has been consistently observed in these patients.
– Abnormal glucose tolerance can be demonstrated in 50% to 70% of patients with CP;
– overt diabetes is present in 32% to 40%.
• Deficits are progressive; if individual patients are repetitively tested, progressive deterioration is
observed.
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SURGICAL PATHOLOGY OF PANCREAS
65
SURGICAL PATHOLOGY OF PANCREAS
Ultrasound is useful in initial evaluation of patients with suspected CP. Supportive findings include:
– atrophy of the gland,
– reduced echogenicity,
– dilation of the pancreatic duct to greater than 4 mm, and
– associated cystic lesions.
• Ultrasound examination of the pancreas may sometimes be compromised by overlying intestinal gas.
• Ultrasound has a reported sensitivity rate of about 60% for CP and a specificity rate of 80% to 90%.
Computed tomographic (CT) examination of the pancreas is more sensitive than ultrasound in CP,
although CT examination involves ionizing radiation and is more expensive.
• CT findings consistent with this diagnosis include glandular atrophy, irregularity of the pancreatic
outline, calcification, and ductal dilation (Fig. 32-3 and Fig. 32-4). Small cystic lesions are well
demonstrated by CT. Sensitivity for CT approaches 75% to 90%, with a specificity of 85%.
ERCP has become widely recognized as the most sensitive and reliable method for diagnosing CP.
• The sensitivity of ERCP approaches 90%, with equal specificity:
– In earliest CP, ductal changes are limited to secondary and tertiary ducts that show irregular dilation.
– In moderate disease, the main pancreatic duct may be dilated with alternating areas of stenosis.
– In advanced CP, marked ductal changes may form a chain-of-lakes appearance.
• ERCP is also useful in demonstrating associated anatomic abnormalities, such as common bile duct
stenosis or pancreatic pseudocyst. Most studies comparing the sensitivity of ERCP and pancreatic
secretory tests have found good correlation in advanced disease. In earlier stages of CP, correlation of
morphologic changes and pancreatic function is often poor.
Pain
Abstinence
• Management of pain in patients with CPshould begin with abstinence. With elimination of alcohol, 50%
to 75% of patients have some decrease in pain, although most do not become pain free. Because
alcohol is a secretagogue for pancreatic enzymes, pain relief is more likely in patients who retain some
exocrine function.
Enzyme Replacement
• Exogenous enzyme administration as a treatment for pain has been proposed, based on the concept
of negative-feedback inhibition of pancreatic secretion.
• In humans, the intraduodenal administration of trypsin or chymotrypsin inhibits pancreatic secretion,
and diversion of pancreatic secretion from the duodenum stimulates secretion of digestive enzymes.
• It has been postulated that patients with CP may have continuous stimulation by hormonal or neural
pathways because of diminished secretion of digestive enzymes.
• Eventually, most patients with chronic pancreatitis require narcotic pain relief; addiction is common
and makes evaluation of treatments aimed at pain relief difficult.
Percutaneous, radiologically guided injection of the celiac ganglia with neural ablative agents
has been used in patients with CP, based on the success of this approach in patients with pancreatic
cancer. The procedure is not usually effective long-term in CP, with pain relief lasting 6 months in
fewer than half of treated patients. Repeated injection is not usually successful.
Surgical Treatment
• Intractable pain is the most frequent indication for operation in patients with CP.
• Operation may be considered when pain is severe enough:
– to interfere substantially with quality of life,
– to interrupt employment or normal family life,
– to affect general health by interfering with nutrition, or
– to cause narcotic addiction.
• All patients being considered for operative treatment should undergo CT examination of the pancreas
to exclude pancreatic carcinoma and ERCP to evaluate pancreatic ductal anatomy.
• Operative treatment for chronic pain associated with CPcan be broadly divided into:
– ductal drainage procedures and
– resection procedures.
Ductal drainage procedures
• Pancreaticojejunostomy. When patients with chronic pancreatitis have pancreatic ducts dilated to
more than 8 mm, ductal decompression using pancreaticojejunostomy (Puestow procedure) may be
employed for relief of pain.
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SURGICAL PATHOLOGY OF PANCREAS
• Pancreatic Resection. Pancreatic drainage is not feasible when pancreatic ducts are small or normal
in diameter. Pancreatic resection may be considered when patients have small ducts, when the
disease process involves primarily one portion of the gland and the remainder of the gland is nearly
normal, or after a failed pancreaticojejunostomy. The rationale for pancreatic resection is that pain and
risk of complications are reduced by removing the diseased portion of the gland.
• Distal pancreatectomy may be performed when pathologic changes are confined to the tail or body of
the pancreas.
• Resection of the pancreatic head by pancreaticoduodenectomy may be appropriate in selected
patients with disease confined predominantly to the head of the gland.
• Indications for pancreaticoduodenectomy include:
(1) a chronic inflammatory mass involving primarily the head of the gland and the uncinate process,
(2) a chronic inflammatory mass in the head of the pancreas associated with duodenal stenosis,
(3) multiple pseudocysts confined to the head of the pancreas, and failure of pancreaticojejunostomy
secondary to inadequate drainage of the uncinate process.
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SURGICAL PATHOLOGY OF PANCREAS
Pancreatico-jejunostomy
72
SURGICAL PATHOLOGY OF PANCREAS
Malabsorption
• Treatment of malabsorption secondary to pancreatic insufficiency requires delivery of exogenous
pancreatic enzymes in active form to the duodenum.
• This goal is often difficult because of inadequate amounts of pancreatic enzymes in commercially
available oral preparations and acid-peptic destruction of ingested enzymes. Because steatorrhea is
most troublesome clinically, the delivery of lipase to the duodenum is the critical variable.
• Fat malabsorption usually does not occur if 25,000 IU of lipase activity can be provided during a 4-
hour postprandial period.
• The major impediment to delivery of oral pancreatic enzymes to the postprandial small intestine is
gastric acidity.
• Pancreatic enzymes are active at an alkaline pH and inactive at a pH of less than 5.
• Pancreatic lipase is irreversibly denatured at a pH of less than 4.
• Several approaches have been used to circumvent gastric effects:
– Large amounts of pancreatic enzymes have been given with meals in the hope that enough
enzyme would survive gastric passage to digest the fat contained in a typical diet.
– Suppression of gastric acid production using histamine-2 (H2) receptor antagonists or antacids
are attempts to protect enzyme activity.
– Enteric-coated formulations of pancreatic enzymes have been prepared that resist acid but
dissolve (and release their contents) on contact with the alkaline pH found in the small intestine.
– Finally, acid-stable forms of lipase with a wider pH range of activity, derived from Aspergillus
niger and Rhizopus arrhizus, have been developed for human testing.
• A rational approach to treatment of pancreatic insufficiency begins with the administration of sufficient
enzyme tablets to abolish azotorrhea and to reduce steatorrhea to tolerable levels. This often means
ingesting several tablets with each meal. If symptoms persist, the number of tablets should be
increased, or the fat content of meals may be decreased. H2 receptor antagonists may be added for
patients resistant to these measures. If steatorrhea persists, a search for other contributing causes
(bacterial overgrowth, ileal disease) should be performed. 74
SURGICAL PATHOLOGY OF PANCREAS
Biliary Complications
• Biliary complications involving the common bile duct can occur in CP because of the intimate
association of that structure with the head of the pancreas. In two thirds of the population, the common bile duct traverses
the pancreatic parenchyma; in about 25%, the common bile duct lies in a groove along the posterior surface of the pancreas; and in only 10%,
the duct is extrapancreatic, always posterior to the gland.
• Thus, fibrosis associated with CPcan encase and compress the common bile duct.
• Common bile duct stenosis is a relatively common complication of CP, occurring in about 10% of cases
observed long-term.
• Alkaline phosphatase elevation is the most sensitive screening method for detection of biliary stenosis,
and a larger proportion of patients demonstrate increases in alkaline phosphatase than develop
jaundice or symptoms of biliary obstruction. Cholangiographic investigation of patients with common
bile duct disease can be accomplished either by transhepatic or retrograde endoscopic routes.
Because CPpatients with common bile duct disease frequently require treatment of concurrent
pancreatic disease, examination of both systems is best accomplished by ERCP. Bile duct fibrosis
typically results in long, gradually tapering strictures conforming to the intrapancreatic duct.
• Malignant strictures usually result in abrupt termination of the biliary duct. The proximal suprapancreatic
portion is variably dilated.
• The most serious sequelae of unrelieved biliary obstruction are cholangitis and biliary cirrhosis.
Collected series of CPpatients with common bile duct stenosis suggest that each of these
complications develops in 7% to 10% of patients with radiographic abnormalities. The degree of biliary
obstruction as shown by cholangiography does not correlate with severity of pancreatic disease, liver
histology, or biochemical abnormalities, and therapeutic decisions should be based on clinical factors
rather than radiologic criteria. Persistent elevation of serum alkaline phosphatase (three to five times
normal), although imperfect, is probably the best predictor of progressive biliary stenosis.
75
SURGICAL PATHOLOGY OF PANCREAS
• Operation in patients with stricture of the common bile duct associated with CP is justified to treat
symptoms or to prevent the development of biliary cirrhosis. Operative indications include the
following:
• Persistent jaundice
• Cholangitis
• Liver biopsy evidence of biliary cirrhosis
• Inability to exclude pancreatic cancer
• Progressive stricture supported by radiologically progressive dilation of extrahepatic and
intrahepatic biliary ducts
• Persistent elevation of alkaline phosphatase at greater than three times normal
• Both choledochoduodenostomy and choledochojejunostomy are excellent operative choices for
patients with intrapancreatic strictures of the common bile duct. A number of other complications can
occur in CP, including pancreatic pseudocyst, pancreatic ascites, and splenic vein thrombosis.
Prognosis
• Patients with CP have decreased long-term survival rates compared with the general population, with
an excess mortality rate of 36% over 20 years.
• Surprisingly, less than 20% of deaths are directly attributable to pancreatitis or its complications.
• Excessive mortality is related to the extrapancreatic complications of alcoholism and smoking.
Cancers of the aerodigestive system, complications of diabetes, and complications of cirrhosis are the
most frequent causes of death.
• Pancreatic cancer has been reported to occur in 4% of patients with CPobserved for 20 years.
76
SURGICAL PATHOLOGY OF PANCREAS
• The management of neoplasms of the exocrine pancreas presents a major challenge to the surgeon.
• The most common of these tumors, ductal adenocarcinoma of the pancreas, has become the fifth
most common cause of cancer death in the United States and continues for the most part to be
recalcitrant to treatment.
• Other less common neoplasms of the pancreas can present formidable difficulties in diagnosis and
treatment.
– The generally vague early symptoms of pancreatic disease,
– The inaccessibility of the organ to examination,
– The aggressiveness of most pancreatic tumors, and
– The technical difficulties associated with pancreatic surgery
make pancreatic exocrine neoplasms among the most daunting diseases treated by surgeons.
Nevertheless, progress has been made in our ability to manage pancreatic neoplasms.
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SURGICAL PATHOLOGY OF PANCREAS
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SURGICAL PATHOLOGY OF PANCREAS
79
SURGICAL PATHOLOGY OF PANCREAS
• The exocrine pancreas contains two major types of epithelium: acinar and ductal.
• The acinar cells of the pancreas are primarily concerned with the elaboration of digestive enzymes,
whereas the ductal epithelium is responsible for the secretion of fluid and electrolytes and the
conveyance of pancreatic juice to the duodenum.
• The ductal type of epithelium begins with the so-called centroacinar cells, which provide an interface to
the acinar cells that cluster around them in a grapelike fashion.
• The smallest pancreatic ducts are lined by low cuboidal epithelium.
• As these ducts coalesce to form progressively larger conduits, the height of the lining cells increases;
• The epithelium becomes columnar in the main pancreatic duct.
• The character of the epithelium also changes in the larger ducts, with an increasing percentage of
mucin-producing cells as the ducts approach the duodenum.
• Despite the fact that ductal epithelial cells make up less than 5% of the pancreatic mass, they appear to
be the cells of origin of most pancreatic carcinomas. Although evidence suggests that acinar epithelium
may dedifferentiate to a ductlike form, the most straightforward interpretation of human pancreatic
carcinogenesis is that the tumors for the most part arise from preexisting ductal cells.
• The microscopic appearance of a typical ductal pancreatic cancer consists of large and small glands
lined by cuboidal or columnar epithelium producing variable amounts of mucin. The glands are
embedded in a dense fibrous matrix, which is responsible for the scirrhous consistency of the tumors.
• The degree of differentiation of ductal carcinoma varies; poorly differentiated tumors demonstrate less
gland formation and mucus production and more epithelial anaplasia.
• Most patients with pancreatic cancer have an associated chronic obstructive pancreatitis, with duct
dilation, atrophy and fibrosis of the acinar parenchyma, and varying degrees of chronic lymphocytic
infiltration. About 10% of patients show histologic evidence of superimposed acute pancreatitis with a
polymorphonuclear cell infiltrate; pseudocyst formation can occur in this group but is rare.
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82
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83
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Body
Head, Body,
Head and Tail and
Tail
Site of Metastasis (n = 106) (n = 34) (n = 24)
Lymph node involvement in duct cell carcinoma of the head of the pancreas 85
SURGICAL PATHOLOGY OF PANCREAS
86
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TNM DEFINITIONS
Primary Tumor
T1 No direct extension of the primary tumor beyond the pancreas
T2 Limited direct extension (to duodenum, bile ducts, or stomach), still possibly permitting tumor resection
T3 Further direct extension, incompatible with surgical resection
TX Direct extension not assessed or not recorded
Distant Metastasis
M0 No distant metastasis
M1 Distant metastatic involvement
MX Distant metastatic involvement not assessed or not recorded
STAGE GROUPING
Stage I T1, T2, N0, M0—No or limited direct extension to adjacent viscera, with no regional node extension and
absence of distant metastases. Limited direct extension defined as involvement of organs adjacent to the
pancreas that could be removed en bloc with thepancreas if a curative resection were attempted.
Stage II T3, N0, M0—Further direct extension of tumor into adjacent viscera, with no lymph node involvement and
no distant metastases, which precluded surgical resection.
Stage III T1-3, N1, M0—Regional node metastases without clinical evidence of distant metastases.
Stage IV T1-3, N0-1, M1—Distant metastatic disease in liver or other sites.
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• Many of the difficulties in the treatment of pancreatic cancer can be traced to our inability to diagnose
the disease in its early stages.
• The vague early symptoms of pancreatic cancer are often minimized by both patient and physician,
leading to a delay of months in making the diagnosis.
• It is ordinarily not until the patient develops jaundice or extreme weight loss that the diagnosis is made, and by
this time the pancreatic tumor is typically large and has grown beyond the confines of the pancreas.
Clinical Symptoms and Signs
• The most common presenting symptoms of pancreatic cancer is weight loss, which is usually
substantial, averaging 10 kg. The weight loss can initially occur as an isolated symptom in the face of a
seemingly normal appetite. Later, it is usually associated with anorexia. Unexplained documented
weight loss should prompt a search for occult malignancy; in older adults, it is appropriate to perform a
CT scan of the abdomen for this indication alone. Other digestive symptoms are common in pancreatic
cancer and include nausea, vomiting, and change in bowel habits.
• Most patients with pancreatic cancer come to physicians because of jaundice. In people older than
60 years of age, the combination of jaundice and weight loss usually means carcinoma of the pancreas
or periampullary region.
• The diagnosis is often evident, requiring only appropriate imaging studies for confirmation.
• The jaundice is progressive. It is associated with dark urine and light stools. Pruritus is present in
about one fourth of jaundiced patients.
• Although it is often taught that carcinoma of the pancreas presents with painless jaundice (to help
distinguish it from choledocholithiasis), this aphorism is not accurate. Most patients do experience pain
as part of the symptom complex of pancreatic cancer.
• Pain is usually perceived in the epigastrium but can occur in any part of the abdomen and can radiate
to the back. Early on, it is often mild and vague, that explain the delay in diagnosis.
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Weight loss 92
Jaundice 82
Pain 72
Anorexia 64
Dark urine 63
Light stools 62
Nausea 45
Vomiting 37
Weakness 35
Pruritus 24
Diarrhea 18
Melena 12
Constipation 11
Fever 11
Hematemesis 8
• 50% to 70% of patients with pancreatic cancer experience pain that they describe as moderate or
severe; the prevalence is particularly high with tumors of the body and tail, probably because of
invasion of the celiac plexus.
• The most common presenting signs of pancreatic cancer are jaundice and hepatomegaly.
• When bilirubin levels are only minimally elevated, icterus may be confined to the sclerae, but most
patients have cutaneous changes by the time of presentation. Hepatomegaly usually reflects
congestion associated with biliary obstruction and does not imply the presence of metastatic disease
unless the liver is nodular or hard.
• The obstructed gallbladder is palpable in about 25% of patients with pancreatic cancer(Courvoisier-
Terrier sign). In most patients, the tumor itself is not palpable.
• Ascites is present in about 15% of patients with pancreatic cancer. With large tumors, there can be
gross or occult blood in the stool from invasion of the duodenum, stomach, or colon.
Laboratory Investigations
• In carcinoma of the head of the pancreas, liver function tests typically reveal :
– elevations in bilirubin (particularly the conjugated fraction) and
– alkaline phosphatase characteristic of extrahepatic biliary obstruction.
– The transaminases can also be elevated, but usually not to the extent of the alkaline
phosphatase. If jaundice has been long-standing,
– the prothrombin time can be abnormally prolonged.
– Mild elevations of the serum amylase in the range of 300 U/L occur, but marked elevations of
serum amylase are rare.
• Routine laboratory determinations add little to the diagnosis of pancreatic cancer other than reinforcing
the suspicion of extrahepatic biliary obstruction.
• Considerable effort has been expended to find serum markers of pancreatic cancer. Mucins are large
glycosylated glycoproteins whose function appears to be protection and lubrication of epithelial cells.
Mucin molecules are produced by most moderately well-differentiated pancreatic carcinomas. 90
SURGICAL PATHOLOGY OF PANCREAS
• In the past few years, mucin-associated antigens have been isolated and purified. CA19-9 is an
example of a mucin-associated carbohydrate antigen that can be detected in the serum of patients with
pancreatic cancer.
• The CA19-9 antigen has been identified as sialosyl-fucosyl-lactotetrose, which corresponds to the
sialylated Lewisa (Lea) blood group substance found on erythrocytes. About 5% of the Western
population lacks the Lewis gene and therefore cannot make CA19-9. The CA19-9 antigen resides in
cell membrane glycolipid and in mucin glycoprotein. CA19-9 can be detected in pancreatic juice, in
serum, and in pancreatic tissue by immunohistologic techniques.
• Serum levels of CA19-9 are elevated (above 37 U/mL) in about 75% of patients with pancreatic cancer.
Unfortunately, CA19-9 is also elevated in about 10% of patients with benign diseases of the pancreas,
liver, and bile ducts.
• The availability of CA19-9 also appears not to have led to earlier diagnosis of pancreatic cancer
because most patients are symptomatic at presentation and CA19-9 is often not elevated in very small
cancers.
• On the other hand, the use of CA19-9 with imaging studies improves overall diagnostic accuracy and
can simplify the evaluation of patients with suspected pancreatic cancer.
• Of potential diagnostic significance is the fact that about 90% of human pancreatic cancers contain the
mutated c-K-ras oncogene.
• The c-K-ras gene is ordinarily present in human cells and encodes a membrane-bound protein that
possesses high affinity for guanosine triphosphate and guanosine diphosphate and appears to be
important for signal transduction across the cell membrane. The ras protein is active when bound to
guanosine triphosphate but is inactive when bound to guanosine diphosphate. Certain mutations in the
gene result in its transformation to an active oncogene. In the case of c-K-ras, a single point mutation at
codon 12 is sufficient. As a result of the mutation, the level of activated ras protein rises, and high
levels of active protein can contribute to unrestrained cellular growth by facilitating the transmission of
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SURGICAL PATHOLOGY OF PANCREAS
Radiologic Investigations
• Imaging of the pancreas has dramatically improved with the development of ultrasonography, CT scanning, and ERCP
MRI(MRCP). With appropriate use of these studies, it should be possible to arrive at a radiologic diagnosis of pancreatic
cancer in over 90% of patients presenting with the disease.
• Standard transcutaneous ultrasonography is an appropriate first test in the evaluation of the patient with jaundice
because the presence of a dilated common bile duct or intrahepatic bile ducts is essentially diagnostic of extrahepatic
biliary obstruction. This finding directs the physician to a search for the cause of the obstruction. If the bile ducts are not
dilated, mechanical obstruction is unlikely, and the diagnostic thrust should move toward hepatocellular disease.
Ultrasonography is also the best test to determine whether gallstones are present; this is extremely important because
choledocholithiasis is one of the conditions most likely to cause jaundice in the elderly population. If an ultrasound
examination shows gallstones and no evidence of a pancreatic mass, the appropriate next step is ordinarily an ERCP
(see later) to document common duct stones. On the other hand, if no gallbladder stones are present by ultrasonography,
choledocholithiasis is unlikely, and a pancreatic or periampullary tumor or chronic pancreatitis becomes a more likely
cause of the obstruction. Ordinarily, a CT scan is the most appropriate next test.
• Ultrasonography reveals a pancreatic mass in 60% to 70% of patients with pancreatic cancer, but the sensitivity of
ultrasound is slightly lower than that of CT, though the absence of a mass on ultrasound scan cannot be accepted as
firm evidence against pancreatic cancer.
• In most cases of pancreatic cancer, CT is the single most useful test.
• CT scanning not only usually detects the presence of the tumor mass but also provides important information about the
extent of the tumor. CT scans may miss tumors smaller than 2 cm. In such cases, the CT findings may be limited to
pancreatic or bile duct dilation. Such findings are highly suspicious for pancreatic malignancy and should be further
evaluated, ordinarily with ERCP. Dynamic CT scanning, in which high-speed scans are obtained during rapid intravenous
administration of iodinated contrast material, and new techniques such as three-dimensional reconstruction from
spiral scans provide excellent information about vascular invasion.
• CT scans provide the best available radiologic information to determine whether or not a pancreatic neoplasm is
resectable, but they cannot be considered absolutely definitive in this regard. Only about half of pancreatic tumors that
appear to be confined to the pancreas on CT scan are found to be resectable in the operating room. 92
SURGICAL PATHOLOGY OF PANCREAS
• CT scanning is more accurate in the diagnosis of unresectability. CT findings that indicate that the
tumor is unlikely to be surgically curable include vascular invasion, enlarged lymph nodes outside the
boundaries of resection, ascites, distant metastases (eg, liver), and distant organ invasion (eg, colon).
• When a CT scan shows distant metastases or extensive local invasion, the positive predictive value of
the technique is high; some 90% of such patients have unresectable disease at laparotomy.
Resectability is a relative term, the definition of which depends on how extensive a surgical resection
is contemplated. For example, portal venous compression is typically considered a sign of
unresectability, yet many surgical groups would consider performing a segmental resection of the
portal vein if the tumor otherwise appeared removable.
• Likewise, some groups advocate an extensive upper abdominal lymph node dissection, which
encompasses nodes well away from the primary lesion.
• Fine-needle aspiration biopsy of the pancreas under CT or ultrasound guidance is an important
advance in the diagnosis of pancreatic cancer. A 22-gauge needle is passed directly into the
pancreatic mass, and a cytologic examination is performed on aspirated cells. The experience with
this technique is extensive, and most centers report 70% to 80% sensitivity and 100% specificity. The
technique is particularly useful in distinguishing chronic pancreatitis from pancreatic cancer and in
providing a tissue diagnosis in patients with advanced disease who are not considered candidates for
palliative or curative surgery.
• ERCP is an excellent diagnostic test for pancreatic cancer, with sensitivities in the range of 90%.
ERCP does not provide any evidence about spread of disease beyond the pancreas. ERCP is
indicated to resolve special problems in the diagnosis of pancreatic cancer. These include primarily
cases with CT evidence of bile duct or pancreatic duct obstruction without a mass, cases in which the
differentiation between chronic pancreatitis and pancreatic cancer is difficult, and cases of
cholelithiasis and bile duct obstruction without a pancreatic mass on ultrasound.
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• Findings on ERCP that suggest pancreatic cancer include irregular pancreatic duct narrowing, displacement of the main
pancreatic duct, destruction or displacement of side branches of the duct, and pooling of contrast material in necrotic
areas of tumor. In most cases, the bile duct portion of the study shows an irregular stenosis with proximal dilation. In
cases in which the differential diagnosis lies between chronic pancreatitis and pancreatic cancer, the distinction can
usually be made because chronic pancreatitis is characterized by multiple or long stenoses of the pancreatic duct. In
contrast, pancreatic cancer typically causes an abrupt focal interruption of the duct.
• Upper gastrointestinal endoscopy is a useful tool in the diagnosis of pancreatic cancer. Endoscopy can be valuable
in finding tumors of the ampulla of Vater or duodenum, which have a considerably better prognosis than pancreatic
cancers. It may be possible to obtain a tissue diagnosis of pancreatic cancer if there is invasion of the duodenum.
Finally, it is possible to estimate the degree of duodenal obstruction in pancreatic cancer, which can have implications
in choosing therapy.
• An imaging technique that shows promise in the diagnosis and staging of pancreatic cancer is endoscopic
ultrasonography. Rotating ultrasound probes at the tip of an upper gastrointestinal endoscope produce a 360-degree
image. Pancreatic carcinomas appear as hypoechoic areas in the pancreatic substance.
• Endoscopic ultrasonography appears to be more sensitive than transcutaneous ultrasound or CT in detecting tumors
smaller than 2.5 cm. It is also sensitive tool for evaluating vascular invasion. It will probably assume a larger role in the
diagnosis and staging of pancreatic cancer as experience increases.
• The generally grim prognosis of pancreatic cancer has led to pessimism on the part of physicians evaluating elderly
patients with jaundice. Because some jaundiced patients have nonmalignant diseases such as choledocholithiasis or
fibrotic strictures of the bile duct due to chronic pancreatitis, and because some have tumors that carry a better
prognosis than ductal carcinoma of the pancreas (such as carcinoma of the bile duct), it is inappropriate to provide only
palliation of jaundice (eg, by endoscopic stent placement) without a tissue diagnosis. The tools are available to achieve
a firm diagnosis in nearly all jaundiced patients and should be used.
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Curative treatment
Surgery
• Surgical resection is the only potentially curative therapy for pancreatic cancer.
• Pancreaticoduodenectomy. Most resectable carcinomas of the pancreas are located in the head of the
gland, probably because the onset of jaundice results in earlier diagnosis than in tumors involving the
body and tail of the gland.
• Whipple and colleagues first described the operation of removal of the head of the pancreas and
duodenum in 1935.
•
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SURGICAL PATHOLOGY OF PANCREAS
• carcinoma has been carefully evaluated in controlled trials performed by the Gastrointestinal Tumor
Study Group.13 This group initially reported that the use of 5-fluorouracil (5-FU) and external-beam
radiotherapy after resection resulted in a therapeutic benefit.
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Serous Cystadenoma
• Serous cystadenomas are usually large, well-circumscribed
(average, 10 cm) tumors that occur most frequently in the body
and tail of the pancreas. The tumors sometimes develop in the
head of the pancreas and can present with obstructive jaundice
owing to compression of the bile duct. They are slightly more
common in women, and most occur in patients older than 50
years of age.
• On cut section, the serous cystadenoma is multiloculated and
consists of many small cysts ranging in diameter from 1 mm to 2
cm, arranged in a honeycomb pattern. The fluid in the cysts is
clear and serous, and no mucin is present. There is typically a
stellate central core of fibrous tissue. Histologically, the tumor
consists of multiple cystic spaces lined by bland cuboidal
epithelium. The cells characteristically contain glycogen.
• Serous cystadenoma usually presents as an abdominal mass
or abdominal pain. The tumor also can be discovered as an
incidental finding.
• CT scanning and ultrasonography are the most useful
diagnostic tests and usually demonstrate a pancreatic mass.
About 30% contain central calcifications. The cysts are often CT scan of large serous cystadenoma of the
too small to appreciate radiologically. pancreas (arrow), demonstrating central stellate
• Serous cystadenoma is a benign neoplasm. scar and tumor calcifications.
• Resection is usually indicated to differentiate the tumor from
other, more dangerous pancreatic pathology, to eliminate
discomfort, or occasionally to relieve biliary or intestinal
obstruction. If removed with a small margin of surrounding
normal pancreas, the tumor should not recur.
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• Internal drainage of a malignant mucinous cystic tumor results in catastrophic tumor dissemination and
should never be performed. With appropriate treatment, all patients with histologically benign tumors
should be cured; for tumors demonstrating malignant change, the 5-year survival rate after surgery is
about 60%.
Pancreatic lymphoma
• Although lymphoma is not a pancreatic exocrine tumor in the true sense, it can arise in the pancreas and
can present as a pancreatic mass lesion.
• About one third of all non-Hodgkin lymphomas include at least microscopic involvement of the pancreas,
• The term pancreatic lymphoma is reserved for those cases in which the lymphoma appears to have
arisen in the pancreas and in which the bulk of the tumor burden is in the pancreas and peripancreatic
tissues.
• Defined in this way, pancreatic lymphoma accounts for 1% to 2% of pancreatic neoplasms and about
1% of non-Hodgkin lymphomas.
• Pancreatic lymphoma usually has a rapid onset, and the tumors attain large size quickly.
• At the time of presentation, pancreatic lymphomas are typically 6 to 10 cm.
• The clinical presentation is similar to that of ductal pancreatic cancer: weight loss and jaundice are the
most common symptoms.
• Pancreatic lymphoma may be difficult to distinguish from pancreatic carcinoma radiographically, except
that the finding of a large tumor without vascular invasion and with extensive lymphadenopathy should
raise the level of suspicion for lymphoma.
• When the diagnosis is suspected, percutaneous needle biopsy of the mass should be performed.
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Incidence
• Neoplasms of the endocrine pancreas(NEP) are rare, with an annual clinically recognized incidence in
the United States of about 5 cases per million persons and per year.
• In unselected autopsy material, however, the prevalence of these tumors approximates 1 per 100
person-years and are typically noted as incidental findings.
• Cells of the pancreatic islets are presumed to originate from neural crest cells.
• Cells of this origin are called amine precursor uptake and decarboxylation - APUD cells, indicating
they have a high amine content of amine, are capable of amine precursor uptake, and contain an amino
acid decarboxylase.
• A generalized derangement of the APUD system can cause abnormalities of multiple endocrine cells as
is observed in multiple endocrine neoplasia (MEN) syndromes.
• Evidence suggests that some APUD cells may not originate from neural crest cells but rather have an
endodermal origin.
• Neoplasms of the endocrine pancreas can be divided into:
– functional and
– nonfunctional varieties.
• Most pancreatic endocrine neoplasms discovered clinically are functional, indicating that they elaborate
one or more hormonal products into the blood, leading to a recognizable clinical syndrome.
• Functional tumors are named according to their predominate clinical syndrome and hormonal product.
• Patients with endocrine tumors of the pancreas with no recognizable clinical syndrome and normal
serum hormone levels (excluding pancreatic polypeptide) are considered to have nonfunctional
pancreatic endocrine tumors.
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• All neoplasms of the endocrine pancreas have a similar light microscopic appearance. Routine
histologic examination does not predict the biologic behavior or the endocrine manifestations of these
neoplasms. Immunofluorescence techniques and the peroxidase-antiperoxidase procedure allow the
demonstration of specific hormones within neoplastic cells. Malignancy is typically determined by the
presence of local invasion that has spread to regional lymph nodes or by the existence of hepatic or
distant metastases.
• Recent observations in the fields of classic and molecular genetics have added to our knowledge of
pancreatic endocrine neoplasms. For example, malignant pancreatic endocrine tumors have been
found to have clonal chromosomal abnormalities in up to half of cases, whereas ras oncogene
mutations are absent in most of these tumors. Gastrinoma has been shown to be associated with
amplification of the HER-2/neu protooncogene, and insulinoma has been shown to highly express
mRNA for the a subunit of Gs protein. Further, sporadic pancreatic endocrine tumors and tumors
arising as a manifestation of MEN I have both been shown to have mutations leading to genetic loss
on chromosome 11, thereby inactivating a putative tumor suppressor gene on that chromosome.
• Three general principles apply to the treatment of patients with suspected functional neoplasms of the
endocrine pancreas. First is the recognition of the abnormal physiology or characteristic syndrome.
Characteristic clinical syndromes are well described for insulinoma, gastrinoma, VIPoma, and
glucagonoma. The somatostatinoma syndrome is nonspecific, much more difficult to recognize, and
exceedingly rare. Second is the detection of hormone elevations in serum by radioimmunoassay.
Radioimmunoassays are widely available for measuring insulin, gastrin, vasoactive intestinal peptide
(VIP), and glucagon. Assays for somatostatin, pancreatic polypeptide, prostaglandins, and other
hormonal markers are not widely available but can be obtained from certain laboratories and
investigators. The third step in patient evaluation involves localizing and staging the tumor in
preparation for possible operative intervention.
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CT - The initial imaging technique recommended for localizing a pancreatic endocrine neoplasm is a
dynamic abdominal computed tomographic (CT) scan with intravenous and oral contrast.
• The accuracy of CT in detecting the primary islet cell tumor varies from about 35% to 85% and
depends largely on the scanning technique and the size and location of the primary tumor (Fig. 1).
• The accuracy of the CT scan in tumor localization is improved by using both oral and intravenous
contrast as well as focused dynamic scanning through the pancreas at 5-mm intervals.
• The CT scan is also used to assess for peripancreatic lymph node enlargement and the presence of
hepatic metastases.
Visceral angiography Should the CT scan fail to detect the primary tumor, the next step in radiograpic
assessment is visceral angiography, focusing on the selective visualization of the arterial supply to
the pancreas and peripancreatic regions.
• The accuracy of angiography in detecting the primary islet cell tumor varies from 45% to 85%,
depending on radiographic technique and expertise, the selectivity of the contrast injection, and the
size and neovascularity of the primary tumor (Fig. 2).
• This technique takes advantage of the unique biology of gastrinoma, in that gastrinoma cells are
known to respond both in vitro and in vivo to secretin with the release of gastrin.
• Secretin is serially injected through an arterial catheter into at least three sites—the splenic,
gastroduodenal, and inferior pancreaticoduodenal arteries. Samples are drawn from an hepatic vein
catheter before and immediately after these three or more arterial secretin injections. The arterial
supply to the occult gastrinoma can be determined based on which selective secretin injection is
followed by a large increment in hepatic vein gastrin concentration.
• The selective arterial secretin stimulation test was more sensitive than portal venous sampling in
localizing gastrinomas, particularly small gastrinomas arising in the duodenum.
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• Surgical exploration for NEP, permits to perform a complete evaluation of the pancreas and
peripancreatic regions:
– The body and tail of the pancreas are exposed by dividing the gastrocolic ligament. This portion of
the pancreas can be partially elevated out of the retroperitoneum by dividing the inferior
retroperitoneal attachments to the gland.
– After elevating the second portion of the duodenum out of the retroperitoneum using the Kocher
maneuver, the pancreatic head and uncinate process are palpated bimanually.
– The liver is carefully assessed for evidence of metastatic disease.
– Potential extrapancreatic sites of tumor are evaluated in all cases, with particular attention paid to
the duodenum, splenic hilum, small intestine and its mesentery, peripancreatic lymph nodes and
the reproductive tract in women.
• One technique that provides additional information in the intraoperative ultrasonography, which can
assist in tumor identification.
• The goals of surgical therapy for pancreatic endocrine neoplasms include:
– controlling symptoms from hormone excess,
– safely resecting maximal tumor mass, and preserving maximal pancreatic parenchyma.
– Management strategies, including preoperative, intraoperative, and postoperative considerations,
vary for the different types of endocrine neoplasms of the pancreas.
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– Adrenal carcinoma
– Hepatocellular carcinoma
– Carcinoid
– Hypopituitarism
– Chronic adrenal insufficiency
– Extensive hepatic insufficiency
– Surreptitious administration of insulin or ingestion of oral hypoglycemic agents
• A common mistake made in evaluating a patient with suspected insulinoma is to begin with an oral
glucose tolerance test.
• Insulinoma is most reliably diagnosed using a monitored fast. During a monitored fast, blood is
sampled every 4 to 6 hours for glucose and insulin determinations and also at the time of symptom
occurrence.
• Hypoglycemic symptoms typically occur when glucose levels are below 50 mg/dL, with concurrent
serum insulin levels often exceeding 25 mU/mL.
• Additional support for the diagnosis of insulinoma comes from the calculation of the insulin/glucose
ratio at different points during the monitored fast.
• Normal persons have insulin/glucose ratios less than 0.3.
• Patients with insulinoma typically demonstrate insulin/glucose ratios greater than 0.4 after a prolonged
fast.
• Other measurable b-cell products synthesized in excess in patients with insulinoma include C peptide
and proinsulin. Elevated levels of both are typically found in the peripheral blood of patients with
insulinoma.
• The possibility of surreptitious insulin or oral hypoglycemic agent administration should be considered
in all patients with suspected insulinoma.
• C-peptide and proinsulin levels are not elevated in patients who self-administer insulin.
• Additionally, patients self-administering either bovine or porcine insulin may demonstrate anti-insulin
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antibodies in circulating blood.
SURGICAL PATHOLOGY OF PANCREAS
• The presence of oral hypoglycemic agents such as sulfonylureas can be assessed using standard
toxicologic screening.
• After confirming the diagnosis of insulinoma by biochemical analyses, the appropriate localization and
staging studies described earlier are performed.
• For insulinoma, the standard imaging studies include:
– abdominal CT,
– endoscopic ultrasound, and
– visceral angiography.
• The treatment of insulinoma is surgical in nearly all cases.
• Insulinomas are found evenly distributed in the pancreas, with one third found in the head and
uncinate process, one third in the body, and one third in the tail of the gland.
• Of patients diagnosed with insulinoma, 90% are found to have benign solitary adenomas amenable to
surgical cure.
• Less than 10% of patients with insulinoma have some form of the MEN I syndrome. In patients with
MEN I, the possibility of multiple insulinomas must be suspected, and the recurrence rate is higher
than in sporadic cases.
• In about 10% of all cases, insulinoma is metastatic to peripancreatic lymph nodes or to the liver,
justifying a diagnosis of malignant insulinoma.
• Small benign insulinomas not close to the main pancreatic duct can be removed by enucleation,39
independent of their location in the gland
• Large insulinomas deep in the head or uncinate process of the pancreas may not be amenable to local
excision and may require pancreaticoduodenectomy.
• In rare instances, patients undergo exploration for insulinoma without definite preoperative tumor
localization.
• At surgery, no tumor can be identified intraoperatively by visualization, palpation, and real-time
ultrasonography.
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• In 1955, Zollinger and Ellison described two patients with severe peptic ulcer disease and pancreatic
endocrine tumors, postulating that an ulcerogenic agent originated from the pancreatic tumor.
• It is currently estimated that 1 in 1000 patients with primary duodenal ulcer disease and 2 in 100
patients with recurrent ulcer after ulcer surgery harbor gastrinomas.
• 75% of gastrinomas occur sporadically, and 25% are associated with the MEN I syndrome.
• In the past, most gastrinomas were found to be malignant, based on the findings of metastatic disease
at the time of workup or exploration. More recently, with increased awareness and earlier screening for
hypergastrinemia, the diagnosis of gastrinoma is made earlier, leading to the discovery of a higher
percentage of benign, curable neoplasms.
• The clinical symptoms of patients with gastrinoma are a direct result of the circulating
hypergastrinemia:
– Abdominal pain and
– Peptic ulceration of the upper gastrointestinal tract are seen in up to 90% of patients.
– Diarrhea in 50% of patients, and about 10% have diarrhea as the solitary symptom.
– Esophageal symptoms or endoscopic abnormalities from gastroesophageal reflux are seen in
over 50% of patients, with esophagitis typically occurring in association with peptic ulcer disease
or diarrhea.
• The diagnosis of gastrinoma should be suspected in several clinical settings, and the liberal use of
serum gastrin measurement for screening is encouraged. The indications for measuring gastrin include
the following:
– Peptic ulcer disease
– Initial diagnosis
– Recurrent ulcer
– Failure of medical therapy
– Postoperative ulcer
– Postbulbar ulcer
– Family history of ulcer disease
– Ulcer with diarrhea
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• Patients whose localization and staging studies indicate unresectable hepatic metastases should undergo
percutaneous or laparoscopically directed liver biopsy for absolute histologic verification. If unresectable gastrinoma is
confirmed, open surgical exploration is not performed, and the patient is maintained on long-term omeprazole therapy.
Virtually all patients can be rendered achlorhydric with an appropriate dose of omeprazole. Noncompliant patients who
refuse to take appropriate doses of omeprazole and who experience complications related to their ulcer diathesis may
require total gastrectomy. Total gastrectomy removes the end organ (parietal cell mass) and was once the procedure of
choice for gastrinoma. Today, its use in patients with gastrinomas has markedly declined.
• In most patients, unresectable disease is not identified by staging studies, and patients should be offered surgical
exploration with curative intent. At the time of exploration, the entire abdomen is carefully assessed for areas of
extrapancreatic and extraduodenal gastrinoma. Most gastrinomas are found to the right of the superior mesenteric
vessels, in the head of the pancreas or the duodenum. This area is called the gastrinoma triangle.
• Intraoperative ultrasonography should be available to assist in tumor localization. In addition, intraoperative upper
endoscopy may help by allowing transillumination of the duodenal wall and identification of small duodenal gastrinomas.
At exploration, any suspicious peripancreatic lymph nodes are excised and submitted for frozen section. Primary
tumors located in the substance of the pancreas that are small (less than 2 cm) and well-encapsulated can be carefully
enucleated. Pancreatic tumors without defined capsules or that are situated deep in the pancreatic parenchyma may
require partial pancreatic resection by either distal pancreatectomy or pancreaticoduodenectomy.
• In the absence of an identifiable pancreatic or duodenal tumor, a longitudinal duodenotomy can be performed at the
level of the second portion of the duodenum to allow for eversion of the duodenum in a search for duodenal
microgastrinomas. Primary gastrinomas identified in the duodenal wall are resected locally with primary closure of the
duodenal defect. In a small percentage of patients, gastrinoma is found only in peripancreatic lymph nodes, with these
lymph nodes harboring the primary tumor. Resection of these apparent lymph node primary gastrinomas has been
associated with long-term eugastrinemia and biochemical cure in up to half of cases.
• Occasionally, preoperative localization studies, such as portal venous gastrin sampling or the selective arterial secretin
stimulation test, localize the tumor in the gastrinoma triangle; however, no tumor may be demonstrable at laparotomy.
• In the face of such a negative exploration, several surgical options are available.
• First, parietal cell vagotomy has been proposed as a way to reduce antisecretory drug dose requirements in patients on
high-dose antisecretory drug therapy but without prior life-threatening complications.
• Total gastrectomy is the second surgical option for patients with negative results on exploration. Although total
gastrectomy was the most reliable way to control ulcer diathesis in the past, the introduction and availability of
omeprazole has drastically reduced the need for total gastrectomy. There may still be a limited role for total
gastrectomy in patients whose tumors cannot be localized if they cannot or will not take adequate doses of omeprazole.
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• Unfortunately, like parietal cell vagotomy, total gastrectomy leaves the primary tumor behind with the
potential for subsequent tumor growth, metastases, and patient death from tumor burden.
• A third surgical option in patients with clear-cut biochemical documentation of hypergastrinemia,
hyperchlorhydria, and tumor localization in the gastrinoma triangle involves blind pancreaticoduodenectomy.
In a small number of patients, these blind resections have yielded pathologically verified primary
gastrinomas in the duodenal wall or the head of the pancreas which were not apparent at laparotomy.
Blind resections should be performed as classic pancreaticoduodenectomies,(without pylorus preserving
technique) including a distal gastric resection, because duodenal gastrinomas may arise close to the
pylorus and be inadvertently left behind with pylorus-sparing pancreaticoduodenectomy.
• In a limited number of cases reported, patients have been rendered eugastrinemic by blind resection, and
most continue to be eugastrinemic at postoperative follow-up.
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• Most patients with incurable metastatic gastrinoma succumb to eventual tumor growth and
dissemination.
• Multiple modalities have been used to treat patients with such metastatic gastrinoma. The overall
objective response rate to chemotherapy appears to be less than 50%.
• A prospective study of monthly cycles of streptozocin, 5-fluorouracil, and doxorubicin in 10 patients
with metastatic gastrinoma showed a partial response rate of 40%, with 60% having no response.
Chemotherapy did not improve the length of survival.
• Hormonal therapy with octreotide has been reported to improve symptoms, reduce hypergastrinemia,
and diminish hyperchlorhydria in patients with metastatic gastrinoma.
• Patients with gastrinoma associated with MEN I present difficult treatment issues. Omeprazole should
be used to control gastric acid hypersecretion. Surgical treatment of hypercalcemia caused by
parathyroid hyperplasia should preceed any surgical treatment of hypergastrinemia. MEN I gastrinoma
typically involves multiple pancreatic or duodenal neoplasms, and careful preoperative and
intraoperative localization techniques are needed to guide resection. In limited numbers of patients,
hypergastrinemia associated with MEN I gastrinoma has been corrected by surgical resection at short
term follow-up. The overall cure rates with MEN I gastrinoma appear to be lower than those with
sporadic gastrinoma.
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• Verner and Morrison are credited with the definition of this secretory-type diarrhea syndrome following
their report of two cases in 1958.
• Synonyms for this syndrome include the WDHA syndrome (watery diarrhea, hypokalemia, and either
achlorhydria or hypochlorhydria) and the pancreatic cholera syndrome.
• Patients characteristically present with
– intermittent severe diarrhea, typically of a watery nature, averaging 5 L/d (Table 34-5).
– Malabsorption and steatorrhea are not common.
– Hypokalemia results from the fecal loss of large amounts of potassium (up to 400 mEq/d), and
low serum potassium levels are associated with
– muscular weakness,
– lethargy, and
– nausea.
– Half of the patients have some degree of hyperglycemia and hypercalcemia, and cutaneous
flushing can be observed in a minority.
• The diagnosis of VIPoma is typically made after excluding other more common causes of diarrhea.
The active agent in the VIPoma syndrome is usually VIP, with a minority of patients having elevations
of other candidate mediators such as peptide histidine-isoleucine or prostaglandins. Because VIP
secretion can be episodic in patients with VIPomas, several fasting VIP levels should be measured
because a single low VIP level does not rule out the syndrome.
• After biochemical documentation of elevated VIP levels, tumor localization and staging begins with
dynamic abdominal CT scan with intravenous and oral contrast. In addition, because 10% of patients
with VIPomas may have extrapancreatic tumors located in the retroperitoneum or thorax, a thoracic CT
scan is indicated if the abdominal scan fails to identify a tumor.
• In most reported cases, the abdominal CT scan identified the tumor, and further imaging studies, such
as visceral angiography or portal venous hormone sampling, were unnecessary.
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• In preparing patients with VIPomas for surgical exploration, fluid and electrolyte balances must be
corrected by vigorous intravenous fluid administration and appropriate electrolyte replacement.
• Therapy with parenterally administered octreotide can be an important adjunct in the preoperative
setting because octreotide leads to a reduction in circulating VIP levels with a resultant decrease in the
volume of diarrhea. Before octreotide was available, corticosteroids and indomethacin were used
preoperatively to control diarrhea and associated fluid and electrolyte losses.
• Surgical excision of the VIPoma is appropriate in all patients with the Verner-Morrison syndrome. Most
VIPomas have been located in the distal pancreas, where they are amenable to resection by distal
pancreatectomy. If no tumor is found in the pancreas, a careful exploration of the retroperitoneum
including both adrenals should be performed.
• Metastatic disease to the lymph nodes and the liver have been reported in half of all cases. In the
presence of metastatic disease, safe palliative debulking of the metastatic tumor is indicated.
• In patients with recurrent or unresectable VIPoma, octreotide therapy is used to reduce circulating VIP
levels and control diarrhea.
• Chemotherapy specific for VIPoma patients has not been studied prospectively, although small
numbers of patients have appeared to partially respond to streptozocin, combination chemotherapy or
interferon.
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• glucagonoma
• somatostatinoma
• calcitoninoma,
• parathyrinoma,
• GRFoma,
• ACTHoma and
• neurotensinoma.
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• Most glucagonomas have been located in the body and tail of the pancreas. These tumors are
typically large and bulky, and surgical resection has required distal pancreatectomy. Metastases have
been found in most patients, and safe debulking of these metastatic lesions should be considered.
• Glucagonoma patients with incurable or recurrent disease appear to have low response rates to
standard chemotherapeutic agents such as streptozocin and dacarbazine. Octreotide can be
successful in reducing elevated glucagon levels and in controlling the hyperglycemia and dermatitis
associated with incurable glucagonoma.
GLUCAGONOMA
Parameter Description
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• The somatostatinoma syndrome is the least common of the five generally accepted functional
pancreatic endocrine neoplasia syndromes, with an estimated annual incidence of less than 1 in 40
million people.
• The clinical features of the somatostatinoma syndrome are nonspecific and include:
– steatorrhea,
– diabetes,
– hypochlorhydria, and
– cholelithiasis (Table 34-8).
• A fasting plasma somatostatin level can be used to confirm the diagnosis of a somatostatinoma.
• While the normal plasma level is below 100 pg/mL, patients with somatostatinoma have been found to
have high levels of circulating somatostatin, often measurable in nanograms per milliliter.
• Most somatostatinomas have been located in the head of the pancreas and the periampullary region.
The most useful test for localization and staging is the abdominal CT scan, which has been used to
identify and stage these typically large tumors.
• Preoperative treatment of patients with somatostatinoma involves treatment of hyperglycemia and
malnutrition.
• Surgery resection for cure has been uncommon because of the presence of metastatic disease in
most cases. Safe resection of the primary tumor and careful debulking of hepatic metastases appear
to be indicated.
• At the time of exploration, cholecystectomy is indicated even in the absence of documented gallstones
because of the concern about the development of cholelithiasis with persistently elevated somatostatin
levels.
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SURGICAL PATHOLOGY OF PANCREAS
SOMATOSTATINOMA
Parameter Description
Symptoms Steatorrhea
Right upper quadrant pain
Diagnostic tests Hyperglycemia
Hypochlorhydria
Gallstones
Serum somatostatin level
Anatomic localization Most in head or uncinate process of
pancreas
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SURGICAL PATHOLOGY OF PANCREAS
• There are several extremely rare clinical syndromes that have been proposed
as candidate functional endocrine syndromes associated with pancreatic
neoplasms (Table 34-9). These include calcitoninoma,89,90 parathyrinoma,91
GRFoma, ACTHoma and neurotensinoma.92 Calcitonin-secreting pancreatic
endocrine neoplasms are associated with watery diarrhea, whereas
parathyrinomas are accompanied by elevations in PTH-related protein with
clinical features of hypercalcemia. GRFoma is marked by elevations of serum
growth hormone–releasing factor (GRF), with clinical features of acromegaly.
ACTHoma has features of Cushing syndrome, with elevated serum
adenocorticotropic hormone (ACTH). Neurotensinoma appears to be
characterized by tachycardia, hypotension, and malabsorption, with elevation of
serum neurotensin. As further cases are reported and clinical experience
broadens, these rare and unusual functional pancreatic exocrine neoplasms
and others may someday be recognized along with the classic five syndromes
of insulinoma, gastrinoma, VIPoma, glucagonoma, and somatostatinoma.
•
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SURGICAL PATHOLOGY OF PANCREAS
• In about a third of patients with neoplasms of the endocrine pancreas, there is no defined clinical syndrome and no lack
of elevated serum insulin, gastrin, VIP, glucagon and somatostatin levels. These patients are considered to have
nonfunctional endocrine neoplasms. The one hormone that may be elevated in the serum in these nonfunctional tumors
is pancreatic polypeptide. It appears to be a marker for some pancreatic endocrine tumors without being the mediator
of any specific pancreatic polypeptide–related clinical syndrome.93 These nonfunctional endocrine neoplasms present
with clinical manifestations such as abdominal pain, weight loss and jaundice resulting from space-occupying lesions in
the pancreas.94,95 These clinical manifestations are similar to those found in patients with ductal adenocarcinoma of
the pancreas. Nonfunctional tumors are most commonly located in the head, neck, or uncinate process of the
pancreas.96 The malignancy rate for these tumors ranges from 50% to 90%. However, in contrast to the poor
prognosis associated with ductal adenocarcinoma of the pancreas, these nonfunctional tumors tend to grow in a more
indolent fashion and to be associated with a longer survival.
• Localization and staging studies are performed in a similar fashion to those performed for patients with the more
common diagnosis of ductal adenocarcinoma of the exocrine pancreas. The abdominal CT scan is used to evaluate the
primary tumor and to assess for hepatic metastases. Preoperative cholangiography may be indicated in the setting of
jaundice with the potential for imaging by endoscopic or percutaneous transhepatic routes. At surgery most of these
nonfunctional neoplasms are larger than 2 cm and are not safely excised by local techniques. Tumors in the head,
neck, or uncinate process of the pancreas typically require pancreaticoduodenectomy for safe resection, whereas
tumors arising in the body or tail of the pancreas are treated by distal pancreatectomy. Patients with unresectable
tumors in the head of the pancreas are candidates for surgical palliation of obstructive jaundice and gastric outlet
obstruction by biliary-enteric and gastroenteric bypass, respectively. The overall 5-year survival rate in all patients with
resected nonfunctional pancreatic neoplasms approaches 50%.97
• In patients with unresectable disease, partial responses to combination chemotherapy have been reported. In a
multicenter trial reported on by Moertel and associates,46 105 patients with advanced islet cell carcinoma, half of whom
had nonfunctional tumors, were randomly assigned to one of three treatment regimens. The lowest response rate
(30%) was seen in the group receiving chlorozotocin alone; an intermediate response rate of 45% was seen in patients
receiving the combination of streptozocin plus 5-fluorouracil; and the highest response rate of 69% was seen in patients
receiving streptozocin plus doxorubicin. The streptozocin-plus-doxorubicin therapy was associated with a significant
survival advantage when compared to the other two treatments. The most common toxic reactions to the chemotherapy
were nausea and vomiting, leukopenia, and mild renal insufficiency.
•
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SURGICAL PATHOLOGY
All neoplasms of the endocrine OF PANCREAS
pancreas have a similar light microscopic
appearance. Routine histologic examination does not predict the biologic
behavior
Cronic or the endocrine manifestations
Pancreatitis Diagnosis of these neoplasms.
Immunofluorescence techniques and the peroxidase-antiperoxidase
procedure allow the demonstration of specific hormones within neoplastic
cells. Malignancy is typically determined by the presence of local invasion
that has spread to regional lymph nodes or by the existence of hepatic or
distant metastases.
Recent observations in the fields of classic and molecular genetics have
added to our knowledge of pancreatic endocrine neoplasms. For example,
malignant pancreatic endocrine tumors have been found to have clonal
chromosomal abnormalities in up to half of cases,2 whereas ras oncogene
mutations are absent in most of these tumors.3 Gastrinoma has been
shown to be associated with amplification of the HER-2/neu
protooncogene,4 and insulinoma has been shown to highly express mRNA
for the a subunit of Gs protein.5 Further, sporadic pancreatic endocrine
tumors and tumors arising as a manifestation of MEN I have both been
shown to have mutations leading to genetic loss on chromosome 11,
thereby inactivating a putative tumor suppressor gene on that
chromosome.6
Three general principles apply to the treatment of patients with suspected
functional neoplasms of the endocrine pancreas. First is the recognition of
the abnormal physiology or characteristic syndrome. Characteristic clinical
syndromes are well described for insulinoma, gastrinoma, VIPoma, and
glucagonoma. The somatostatinoma syndrome is nonspecific, much more
difficult to recognize, and exceedingly rare. Second is the detection of
hormone elevations in serum by radioimmunoassay. Radioimmunoassays
are widely available for measuring insulin, gastrin, vasoactive intestinal 133
peptide (VIP), and glucagon. Assays for somatostatin, pancreatic