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Introduction.
The Basis of Biochemistry.
OUTLINE
Biochemistry Definitions
Functional groups of
the biomolecules
1. INTRODUCTION TO BIOCHEMISTRY
Functional groups of
the biomolecules
1. INTRODUCTION TO BIOCHEMISTRY
2. WATER AS A SUSTAINING LIFE MEDIUM
Maximum density at 4 °C
High boiling point
specific heat
heat of vaporisation
thermal conductivity
dielectric constant
capacity to form hydration shells surrounding ions
Solvent of amphipathic molecules
of polar and non ionic compounds
High surface tension
Transparency
2. WATER AS A SUSTAINING LIFE MEDIUM
Acid-Base equilibrium
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3. BIOENERGETICS
DGo’ = - RT ln K’eq
DG = DGo’ + RT ln ([C][D]/[A][B])
• The useful free energy from an ATP molecules is store in the two
pyrophosphoryl (phosphoric acid anhydride linkages)
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3. BIOENERGETICS
Biochemical reactions are coupled
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3. BIOENERGETICS
ATP
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3. BIOENERGETICS
ATP
• It is an intermediate energy shuttle
molecule (connect catabolism and
anabolism)
• It is involved in several processes:
• Biomolecules synthesis
• Active transport through the
membranes
• Mechanic work (i.e. muscular
contraction)
• It transfers phophoryl groups from high
energy compounds to less energetic
compounds.
• Group transfer potential: ATP hydrolysis
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3. BIOENERGETICS
ATP
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3. BIOENERGETICS
Phosphoryl group transfer potential
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3. BIOENERGETICS
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3. BIOENERGETICS
Electron transporters
Nicotinamide coenzymes
They transport 2 electrons
They derive from niacin (nicotinic acid) (B3) to provide
nicotinamide portion
They transfer hydride (H-) to and from the reactants
– Nicotinamide adenine dinucleotide (NAD+)
– Nicotinamide adenine dinucleotide phosphate (NADP+)
Electron transporters
Nicotinamide coenzymes
Nicotinamide
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3. BIOENERGETICS
Electron transporters
Nicotinamide coenzymes
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½ O2 + NADH + H+ H2O + NAD+ DE0’= 0.82-(-0.32) = 1.14 V
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